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Construction of a High-efficiency Shuttle Vector Containing the MinimalReplication Origin of Bacillus thuringiensis

최재영|왕용|김양수|신상철|최희규|제연호|진병래|노종열|강중남|Ming Shun Li
한국잠사학회 (Korean Society Of Sericultural Science)|2005.12|International Journal of Industrial Entomology and Biomaterials|제11권 제2호|pp. 125-127

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Abstract

In order to improve the transformation efficiency of the Bacillus thuringiensis (Bt)-Escherichia coli (E.coli) shuttle vector, pHT3101, we intended to minimize replication origin of Bt in pHT3101. For this, two modified shuttle vectors, pHT1K and pHT261, in which 2.9 kb of replication origin of Bt were shortened to 1 kb and 261 bp, respectively as previously reported. Whereas the pHT1K could efficiently transform Bt into the antibiotic resistant, no transformants were obtained with pHT261. Furthermore, pHT1K showed higher transformation efficiency compared to that of parent vector, pHT3101. Therefore, pHT1K might be a very useful Bt-E. coli shuttle vector carrying minimal replication origin of Bt.

참고문헌 (12)

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