본문 바로가기
KERD
논문KCI 등재

Molecular Characterization of a Novel Bacillus thuringiensis Strain from China

최재영|왕용|김양수|최희규|제연호|Ming Shun Li|Xu Feng Qi|Ji Zhen Song|강남중|Jian Hong Li1
한국잠사학회 (Korean Society Of Sericultural Science)|2005.09|International Journal of Industrial Entomology and Biomaterials|제11권 제1호|pp. 57-61

2

조회

0

다운로드

1

인용

0

참고문헌

초록

Abstract

A strain of Bacillus thuringiensis that showed significantly high toxicity to Plutella xylostella was isolated from a dust sample collected from Chinese tobacco warehouse and characterized. The isolate named B. thuringiensis LY-99 was determined to belong to subsp. alesti (H3a3c) by an H antisera agglutination test and produced bipyramidal inclusions. Plasmid and crystal protein patterns of the LY-99 were different from those of the reference strain, subsp. alesti.PCR analysis with specific primers revealed that this isolate contained abundant cry genes including cry1Aa, cry1Ac, cry1B, cry1D, cry1E, cry1F and cry2 genes,which was absolutely different from cry gene profile of the subsp. alesti. In addition, insecticidal activity of the LY-99 against P. xylostella larvae was about 44 times higher than that of the subsp. alesti.

참고문헌 (26)

  1. N. B, "Prediction of insecticidal activity of Bacillusthuringiensis strains by polymerase chain reaction productprofiles. Appl. Environ. Microbiol. 57", pp.3057~3061 (1991)
  2. Ceron, "Specific PCR primers detected to identify cryI andcryIII gene within a Bacillus thuringiensis strain collection.", 61, pp.3826~3831 (1995), L. Guereca and A. Bravo
  3. Ceron, "PCR analysis of thecry1 insecticidal crystal family genes from Bacillus thuringiensis.Appl. Environ. Microbiol. 60", pp.353~356 (1994)
  4. Chang, "Isolation of a strain of Bacillusthuringiensis ssp. kurstaki HD-1 encoding δ", 26, pp.387~390 (1998), -endotoxinCry1E.
  5. Ferre, "Resistance to the Bacillus thuringiensis bioinsecticidein a field population of Plutella xylostella is due to achange in a midgut membrane receptor. Proc. Natl. Acad.Sci. USA 88", pp.5119~5123 (1991)
  6. Gleave, "Screeningby polymerase chain reaction of Bacillus thuringiensis serotypesfor the presence of cryV-like insecticidal protein genesand characterization of cryV gene cloned from B. thuringiensissubsp. kurstaki. Appl. Environ. Microbiol. 59", pp.1683~1687 (1993)
  7. Groot,A. T, "Insect-resistant transgenicplants in a multi-trophic context.", 31, pp.387~406 (2002)
  8. Herron, "Insecticideresistance in Aphis gossypii glover a serious threat to Australian cotton.", 40, pp.85~91 (2001)
  9. Hofte,H, "Insecticidal crystal proteinsof Bacillus thuringiensis. Microbiol. Rev. 53", pp.242~255 (1989)
  10. Huang, "Cloning, characterizationand expression of a new cry1Ab gene from Bacillusthuringiensis WB9", 26, pp.1557~1561 (2004)
  11. Kalman, "Cloning of a novel cryIC-type gene from a strain of Bacillusthuringiensis subsp. galleriae. Appl. Environ. Microbiol. 59", pp.1131~1137 (1993)
  12. Knowles,B. H, "Mechanism of action of Bacillus thuringiensisinsecticidal delta-endotoxins. Adv. Insect Physiol.24", pp.275~308 (1994)
  13. Laemmli,U. K, "Cleavage of structural proteins duringthe assembly of the head of bacteriophage T4. Nature 227", pp.680~685 (1970)
  14. Lecadet, M. M, "Updating the H-antigenclassification of Bacillus thuringiensis", 86, pp.660~672 (1999)
  15. Lee,C. S, "Cloning and analysis ofdelta-endotoxin genes from Bacillus thuringiensis subsp.alesti. J. Bacteriol. 173", pp.6635~6638 (1991)
  16. Leroy, T, "Genetically modified coffee plants expressingthe Bacillus thuringiensis cry1Ac gene for resistance to leafminer", 19, pp.382~389 (2000)
  17. Li, "Crystal structure ofinsecticidal a-endotoxin from Bacillus thuringiensis at 2.5 Aresolution. Nature 353", pp.815~821 (1991)
  18. Li, J, "Structure of the mosquitocidala-endotoxin CytB from Bacillus thuringiensis sp.kyushuensis and implication for membrane pore formation", 257, pp.129~152 (1996)
  19. Li, M. S, "Isolation and characterizationof a strain of Bacillus thuringiensis ssp. kurstaki containing anew δ-endotoxin gene", 45, pp.299~302 (2002)
  20. Lise, J, "Transgenic plants for insect resistance.", 131, pp.1344~1350 (1998)
  21. Ohba,M, "Serological identication ofBacillus thuringeinsis and related bacteria isolated in Japan.J. Invertebr. Pathal. 32", pp.303~309 (1978)
  22. Russell, "a new computer program for probit analysis. Bull. Entomol.Soc. Am. 23", pp.209~213 (1977)
  23. Schnepf, E, "Bacillus thuringiensisand its pesticidal crystal proteins", 62, pp.775~806 (1998)
  24. Tabashnik, B. E, "Cross-resistance of pink bollworm (Pectinophoragossypiella) to Bacillus thuringiensis toxins", 66, pp.4582~4584 (2000)
  25. Thomas, "effects on insect andmammalian cells in vitro and in vivo. J. Cell. Sci. 60"
  26. Zhang, H. Y, "Compositionand ecological distribution of cry proteins and their genotypesof Bacillus thuringiensis isolates from warehouses inChina", 76, pp.191~197 (2000)

관련 논문AI추천

Characteristics of Hemolysin in MosquitocidalBacillus thuringiensis strain 21-2김광현, 김병우, 김영희, 김위종한국미생물·생명공학회 · 한국미생물·생명공학회지2002 · 제30권 제3호Characterization of a Novel cry1-Type Gene from Bacillus thuringiensis subsp. alesti Strain LY-99왕용, 노종열, 제연호, 진병래, 최재영, Jian Hong Li, Ji Zhen Song, Ming Shun Li, Xu Feng Qi한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2009 · 제18권 제1호항선충성 Bacillus thuringiensis 108균주의 분리와 특성김광현, 류은주, 이재훈한국미생물·생명공학회 · 한국미생물·생명공학회지2007 · 제35권 제3호한국에서 분리한 Bacillus thuringiensis 균주의 cry형 유전자의 동정강은영, 권혁한, 김의나, 박수일, 안성규, 이광용, 이형환한국미생물·생명공학회 · 한국미생물·생명공학회지2005 · 제33권 제2호Construction of a High-efficiency Shuttle Vector Containing the MinimalReplication Origin of Bacillus thuringiensis왕용, 강중남, 김양수, 노종열, 신상철, 제연호, 진병래, 최재영, 최희규, Ming Shun Li한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2005 · 제11권 제2호Expression of a Fusion Protein with Cry1Ac Protein and a Scorpion Insect Toxin in Acrystalliferous Bacillus thuringiensis Strain노종열, 부경생, 신상철, 심희진, 장진희, 제연호, 최재영, Ming Shun Li한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2004 · 제8권 제1호

이 간행물의 다른 논문 (11건)

Food Ingestion, Assimilation and Conversion Efficiency of Mulberry Silkworm, Bombyx mori L.S. Z. Haque Rufaie, V. K. Rahmathulla, R. K. Rajan, G. S. Vindya, M. T. Himantharajpp. 1-12Biotechnological Approaches in Sericultural Science and Technology of UzbekistanShukhrat R. Madyarovpp. 13-19CO2-Narcosis Time Favorable for Colony Development in the Bumblebee Queen, Bombus terrestris김삼은, 이상범, 윤형주pp. 21-25Development of New Productive Bivoltine Silkworm (Bombyx mori L.) Hybrid, APS83 X APS102Chandrashekharaiah, M. Ramesh Babu, H. Lakshmi, J. Prasad, J. Seetharamulupp. 27-36Amino Acid Alterations in the β-tubulin of Metarhizium anisopliae that Confer Benomyl Resistance노종열, 최재영, 제연호, 진병래, 심희진, Ming Shun Li, 부경생, 김순기pp. 37-42
원문 보기