Comparative Transcriptome Analysis of Queen, Worker, and Larva of Asian Honeybee, Apis cerana
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The Asian honeybee, Apis cerana, is a native honeybee species in Korea which is importantin agriculture for pollination and honey production. For better understanding of the physiologyof A. cerana, high-throughput Illumina transcriptome sequencing was performed to analyzethe gene expression profiles of queen, worker, and larva. A total of 219,799,682 clean readscorresponding to 22.2 Gb of nucleotide sequences was obtained from the whole body totalRNA samples. The Apis mellifera reference mRNA sequence database was used to measurethe gene expression level with Bowtie2 and eXpress software, and the Illumina short readswere then mapped to 11,459 out of 11,736 A. mellifera reference genes. Total of 9,221genes with FPKM value greater than 5 of each sample group were subjected to eggNOGwith BLASTX for gene ontology analysis. The differential gene expression between queenand worker, and worker and larva were analyzed to screen the overexpressed genes in eachsample group. In the queen and worker sample group, total of 1,766 genes were differentiallyexpressed with 887 and 879 genes overexpressed over two folds in queen and worker,respectively. In the worker and larva sample group, total of 1,410 genes were differentiallyexpressed with 1,009 and 401 genes overexpressed over two folds in worker and larva,respectively.
Abstract
The Asian honeybee, Apis cerana, is a native honeybee species in Korea which is importantin agriculture for pollination and honey production. For better understanding of the physiologyof A. cerana, high-throughput Illumina transcriptome sequencing was performed to analyzethe gene expression profiles of queen, worker, and larva. A total of 219,799,682 clean readscorresponding to 22.2 Gb of nucleotide sequences was obtained from the whole body totalRNA samples. The Apis mellifera reference mRNA sequence database was used to measurethe gene expression level with Bowtie2 and eXpress software, and the Illumina short readswere then mapped to 11,459 out of 11,736 A. mellifera reference genes. Total of 9,221genes with FPKM value greater than 5 of each sample group were subjected to eggNOGwith BLASTX for gene ontology analysis. The differential gene expression between queenand worker, and worker and larva were analyzed to screen the overexpressed genes in eachsample group. In the queen and worker sample group, total of 1,766 genes were differentiallyexpressed with 887 and 879 genes overexpressed over two folds in queen and worker,respectively. In the worker and larva sample group, total of 1,410 genes were differentiallyexpressed with 1,009 and 401 genes overexpressed over two folds in worker and larva,respectively.
참고문헌 (14)
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