Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana
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The p38 mitogen-activated protein kinase (MAPK) is involved in various processes, including stress responses, development,and differentiation. However, little information on p38 MAPK in insects is available. In this study, a p38 MAPK gene,Accp38b, was isolated from Apis cerana cerana and characterized. The quantitative real-time PCR (Q-PCR) analysis revealed that Accp38b was induced by multiple stressors. Notably, the expression of Accp38b was relatively higher in the pupae phase than in other developmental phases. During the pupae phase, Accp38b expression was higher in the thorax than in the head and abdomen and higher in the fat body than in the muscle and midgut. Immunohistochemisty showed significant positive staining of Accp38b in sections from the brain, eyes, fat body, and midgut of A. cerana cerana. These results suggest that Accp38b may play a crucial role in stress responses and have multiple aspects function during development. [BMB reports 2012; 45(5): 293-298]
Abstract
The p38 mitogen-activated protein kinase (MAPK) is involved in various processes, including stress responses, development,and differentiation. However, little information on p38 MAPK in insects is available. In this study, a p38 MAPK gene,Accp38b, was isolated from Apis cerana cerana and characterized. The quantitative real-time PCR (Q-PCR) analysis revealed that Accp38b was induced by multiple stressors. Notably, the expression of Accp38b was relatively higher in the pupae phase than in other developmental phases. During the pupae phase, Accp38b expression was higher in the thorax than in the head and abdomen and higher in the fat body than in the muscle and midgut. Immunohistochemisty showed significant positive staining of Accp38b in sections from the brain, eyes, fat body, and midgut of A. cerana cerana. These results suggest that Accp38b may play a crucial role in stress responses and have multiple aspects function during development. [BMB reports 2012; 45(5): 293-298]
참고문헌 (26)
- Davis, M. M., "A member of the p38 mitogen-activated protein kinase family is responsible for transcriptional induction of Dopa decarboxylase in the epidermis of Drosophila melanogaster during the innate immune response", Mol. Cell. Biol., 28, pp.4883~4895 (2008)
- Park, J. S., "The role of p38b MAPK in age-related modulation of intestinal stem cell proliferation and differentiation in Drosophila", AGING-US, 1, pp.637~651 (2009), Impact Journal LLC
- Raman, M., "Differential regulation and properties of MAPKs", Oncogene, 26, pp.3100~3112 (2007)
- Ono, K., "The p38 signal transduction pathway: activation and function", Cell Signal, 12, pp.1~13 (2000)
- Han, J., "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells", Science, 265, pp.808~811 (1994)
- Zarubin, T., "Activation and signaling of the p38 MAP kinase pathway", Cell. Res., 15, pp.11~18 (2005)
- An, H., "LZAP inhibits p38 MAPK (p38) phosphorylation and activity by facilitating p38 association with the wild-type p53 induced phosphatase 1 (WIP1)", PLoS One, 6, pp.e16427~ (2011)
- Gong, X., "Mechanisms regulating the nuclear translocation of p38 MAP kinase", J. Cell. Biochem., 110, pp.1420~1429 (2010)
- Roux, P. P., "ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions", Microbiol. Mol. Biol. Rev., 68, pp.320~344 (2004)
- Chen, J., "Participation of the p38 pathway in Drosophila host defense against pathogenic bacteria and fungi", Proc. Natl. Acad. Sci. U.S.A., 107, pp.20774~20779 (2010)
- Craig, C. R., "A Drosophila p38 orthologue is required for environmental stress responses", EMBO Rep., 5, pp.1058~1063 (2004)
- Adachi-Yamada, T., "p38 mitogen-activated protein kinase can be involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis", Mol. Cell. Biol., 19, pp.2322~2329 (1999)
- Park, J. S., "Regulation of the Drosophila p38b gene by transcription factor DREF in the adult midgut", BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 1799, pp.510~519 (2010), Elsevier BV
- Ishida, M., "Involvement of cAMP response element-binding protein in the regulation of cell proliferation and the prolactin promoter of lactotrophs in primary culture", Am. J. Physiol. Endocrinol. Metab., 293, pp.E1529~E1537 (2007)
- Carlezon, W. A., Jr., "The many faces of CREB", Trends. Neurosci., 28, pp.436~445 (2005)
- Han, Z. S., "A conserved p38 mitogen- activated protein kinase pathway regulates Drosophila immunity gene expression", Mol. Cell. Biol., 18, pp.3527~3539 (1998)
- 최강열, "Molecular cloning and characterization of a Drosophila p38 mitogen-activated protein kinase", JOURNAL OF BIOLOGICAL CHEMISTRY, 273, pp.369~374
- Cancino-Rodezno, A., "The mitogen-activated protein kinase p38 is involved in insect defense against Cry toxins from Bacillus thuringiensis", Insect. Biochem. Mol. Biol., 40, pp.58~63 (2010)
- Okamura, T., "ATF-2 regulates fat metabolism in Drosophila", Mol.Biol. Cell, 18, pp.1519~1529 (2007)
- Borders, A. S., "The p38α mitogen-activated protein kinase as a central nervous system drug discovery target", BMC Neurosci., 9(Suppl 2), pp.S12~ (2008)
- Takeda, K., "Neuronal p38 MAPK signalling: an emerging regulator of cell fate and function in the nervous system", Genes Cells, 7, pp.1099~1111 (2002)
- Oliveira, C. S., "The activation of ERK1", 2 and p38 mitogen-activated protein kinases is dynamically regulated in the developing rat visual system, 2008, pp.26, 355~362
- Matsukage, A., "The DRE", DREF transcriptional regulatory system: amaster key for cell proliferation, 2008, pp.1779, 81~89
- Park, S. Y., "Transcriptional regulation of the Drosophila catalase gene by the DRE", DREF system, 2004, pp.32, 1318~1324
- Fei Meng, "Characterization of the TAK1 gene in Apis cerana cerana (AccTAK1) and its involvement in the regulation of tissue-specific development", BMB Reports, 44(3), pp.187~192 (2011), 생화학분자생물학회
- Tufail, M., "Molecular cloning, transcriptional regulation, and differential expression profiling of vitellogenin in two wing-morphs of the brown planthopper, Nilaparvata lugens Stal (Hemiptera: Delphacidae)", Insect. Mol. Biol., 19, pp.787~798 (2010)