Microsatellite Analysis of Silkworm Strains (Bombyx mori) of Japan Origin Preserved in Korea
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
In order to understand the diversity and genetic relationships of silkworm strains preservedin Korea, we genotyped 78 Bombyx mori strains (Bombycidae: Lepidoptera) originating fromJapan, using eight polymorphic microsatellite loci. We obtained per-locus allele numbersranging from 5 to 16 (with an average value of 9.1), per-locus observed heterozygosityranging from 0.13 to 1.00, and per-locus polymorphic information content ranging from 0.36to 0.77, indicating that some loci are highly variable. Phylogenetic analysis with the eightconcatenated microsatellite loci showed no clustering based on known strain characteristicsand origin. Nineteen strain-specific apomorphic alleles, which discriminated 16 of the 78silkworm strains, were obtained from eight loci. These strain-specific alleles can thus beutilized for routine discrimination of strains from Japan, without any further typing of otherloci. Homozygotes were also observed at some loci (27 of 118 genotypes), which can alsobe used to discriminate several strains by typing a few loci. These results showed that eightmicrosatellite loci described herein were sufficiently variable to discriminate among the 78silkworm strains we examined, and may be useful for future investigations of this economically important species.
Abstract
In order to understand the diversity and genetic relationships of silkworm strains preservedin Korea, we genotyped 78 Bombyx mori strains (Bombycidae: Lepidoptera) originating fromJapan, using eight polymorphic microsatellite loci. We obtained per-locus allele numbersranging from 5 to 16 (with an average value of 9.1), per-locus observed heterozygosityranging from 0.13 to 1.00, and per-locus polymorphic information content ranging from 0.36to 0.77, indicating that some loci are highly variable. Phylogenetic analysis with the eightconcatenated microsatellite loci showed no clustering based on known strain characteristicsand origin. Nineteen strain-specific apomorphic alleles, which discriminated 16 of the 78silkworm strains, were obtained from eight loci. These strain-specific alleles can thus beutilized for routine discrimination of strains from Japan, without any further typing of otherloci. Homozygotes were also observed at some loci (27 of 118 genotypes), which can alsobe used to discriminate several strains by typing a few loci. These results showed that eightmicrosatellite loci described herein were sufficiently variable to discriminate among the 78silkworm strains we examined, and may be useful for future investigations of this economically important species.
참고문헌 (21)
- Bostein D, "Construction of a genetic linkage map in man using restriction fragment length polymorphism", Am J Hum Genet, 32, pp.314~331 (1980)
- Dharma Prasad M, "Survey and analysis of microsatellites in the silkworm, Bombyx mori: frequency, distribution, mutations, marker potential and their conservation in heterologous species", Genetics, 169, pp.197~214 (2005)
- Itoh M, "Cloning and sequence analysis of membrane-bound alkaline phosphatase cDNA of the silkworm", Bombyx mori. Biochim Biophys Acta, 1129, pp.135~138 (1991)
- Jin L, "Estimation of genetic distance and coefficient of gene diversity from single-probe multilocus DNA fingerprinting data", Mol Biol Evol, 11, pp.120~127 (1994)
- Kawabata T, "Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologues to arthropod hemocyanin", Proc Natl Acad Sci, 92, pp.7774~7778 (1995)
- 김기영, "Microsatellite analysis of the silkworm strains (Bombyx mori): high variability and potential markers for strain identification", Genes & Genomics, 32(6), pp.532~543 (2010), 한국유전학회
- Kee Young Kim, "Microsatellite Analysis of the Silkworm Strains (Bombyx mori) Originated from China", International Journal of Industrial Entomology, 25(1), pp.81~92 (2012), 한국잠사학회
- Kwok S, "Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus 1 model studies", Nucleic Acids Res, 18, pp.999~1005 (1990)
- Li Y-C, "Microsatellites:genomic distribution, putative functions and mutational mechanisms:a review", Mole Ecol, 11, pp.2453~2465 (2002)
- Li M, "Genetic diversity among silkworm (Bombyx mori L., Lep., Bombycidae) germplasms revealed by microsatellites", Genome, 48, pp.802~810 (2005)
- Principles and practices in sericulture, "Lim SH", National Sericulture and Entomology Research Institute, 117~231 (1996)
- Liu C, "PowerMarker: integrated analysis environment for genetic marker data", Bioinformatics, 21, pp.2128~2129 (2005)
- Meglécz E, "Microsatellite flanking region similarities among different loci within insect species", Insect Mol Biol, 16, pp.175~185 (2007)
- Michaille JJ, "The complete sequence of mag, a new retrotransposon in Bombyx mori", Nucleic Acids Res, 18, pp.674~ (1990)
- Nei M, "Estimation of average heterozygosty and genetic distance from a small number of individuals", Genetics, 89, pp.583~590 (1987)
- Ohta T, "Cloning of a cDNA for DNA supercoiling factor reveals a distinctive Ca2+-binding protein", J Biol Chem, 270, pp.15571~15575 (1995)
- Reddy KD, "Microsatellites in the silkworm, Bombyx mori: abundance, polymorphism, and strain characterization", Genome, 42, pp.1057~1065 (1999)
- Tautz D, "Simple sequences are ubiquitous repetitive components of eukaryotic genomes", Nucleic Acids Res, 12, pp.4127~4138 (1984)
- Weber JL, "Abundant class of human DNA polymorphisms", Genomics, 44, pp.388~396 (1989)
- Weir BS, "Genetic Data Analysis II" (1996), Sinauer Associates, Inc
- Yue GH, "Rapid isolation and characterization of microsatellites from the genome of Asian arowana (Scleropages formosus, Osteoglossidae, Pisces)", Mole Eco, 9, pp.1007~1009 (2000)