Detection of Insect Pathogen Serratia marcescens in Protaetia brevitarsis seulensis (Kolbe) from Korea
2
조회
0
다운로드
13
인용
0
참고문헌
키워드
초록
Protaetia brevitarsis seulensis (Kolbe) is widely used in Korea, as a protein-rich, alternate,functional food with pharmacological benefits. In addition to anti-oxidant properties, the larvaeof P. b. seulensis also show positive effects against hepatic disorder and diabetes; therefore,P. b. seulensis larvae are being reared on a large scale in Korea. We evaluated reared larvaeof P. b. seulensis from Gyeong-gi in Korea. Using 16SrRNA PCR, electro-microscopy, andbioassay techniques, we found that the larvae harbored Spo-1, a bacterium identified as theinsect pathogen Serratia marcescens . Therefore, we highlight the use of this insect as analternate food and the need for its sanitary rearing conditions, as contamination may affectpublic health.
Abstract
Protaetia brevitarsis seulensis (Kolbe) is widely used in Korea, as a protein-rich, alternate,functional food with pharmacological benefits. In addition to anti-oxidant properties, the larvaeof P. b. seulensis also show positive effects against hepatic disorder and diabetes; therefore,P. b. seulensis larvae are being reared on a large scale in Korea. We evaluated reared larvaeof P. b. seulensis from Gyeong-gi in Korea. Using 16SrRNA PCR, electro-microscopy, andbioassay techniques, we found that the larvae harbored Spo-1, a bacterium identified as theinsect pathogen Serratia marcescens . Therefore, we highlight the use of this insect as analternate food and the need for its sanitary rearing conditions, as contamination may affectpublic health.
참고문헌 (25)
- Ajithkumar B, "Spore-forming Serratia marcescens subsp. sakuensis subsp. nov., isolated from a domestic wastewater treatment tank", Int J Syst Evol Microbiol, 53(1), pp.253~258 (2003)[DOI]
- Arthur I. Aronson Wb, "Bacillus thuringiensis and related insect pathogens", Microbiol Rev, pp.1~24 (1986)
- Burnside CE, "A Septicemic condition of adult bees", J Econ Entomol, 21, pp.379~386 (1928)
- Carol R. Lauzon TGB, "Serratia marcescens as a bacterial pathogen of rhagoletis pomonella flies", Eur J Entomol, 100, pp.87~92 (2003)
- Casper Flyg KKaJGB, "Insect pathogenic properties of serratia marcescens phage resistant mutants witha decreased resistance to cecropia immunity and a decreased virulence to drosophila", J Gen Microbiol, 120, pp.173~181 (1980)
- El-Aasar A.M, "Biological and biochemical effects of bacillus thuringiensis serratia marcescens and teflubenzuron and their sequential combined effects on cotton leafworm spodoptera littoralis", Egypt Acad J Biolog Sci, 5(1), pp.1~13 (2013)
- Fedrigo GV, "Serratia marcescens is able to survive and proliferate in autophagic-like vacuoles inside non-phagocytic cells", PloS one, 6(8), pp.e24054~ (2011)[DOI]
- Gerc AJ, "The insect pathogen Serratia marcescens Db10 uses a hybrid nonribosomal peptide synthetase-polyketide synthase to produce the antibiotic althiomycin", PloS one, 7(9), pp.e44673~ (2012)[DOI]
- Grimont F, "The Genus Serratia" (2006)[DOI]
- Hurst MR, "Isolation and characterization of the Serratia entomophila antifeeding prophage", FEMS Microbiol Lett, 270(1), pp.42~48 (2007)[DOI]
- Hurst MR, "Induced expression of the Serratia entomophila Sep proteins shows activity towards the larvae of the New Zealand grass grub Costelytra zealandica", FEMS Microbiol Lett, 275(1), pp.160~167 (2007)[DOI]
- Jang-Jih Lu, "Use of pcr with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid", J Clin Microbiol, 38(6), pp.2076~2080 (2000)
- Joyner J, "Use of Quantitative Real-Time PCR for Direct Detection of Serratia marcescens in Marine and Other Aquatic Environments", Appl Environ Microbiol, 80(5), pp.1679~1683 (2014)[DOI]
- 정지아, "Serratia marcescens 검출을 위한 PCR 기법 개발 및 돼지정액 유래균주에 대한 항생제 감수성 양상", 생명과학회지, 23(9), pp.1133~1139 (2013), 한국생명과학회[DOI]
- 권은영, "흰점박이꽃무지(Protaetia brevitarsis)의 식품원료화를 위한 전처리 조건 확립", 한국식품영양과학회지, 42(3), pp.397~402 (2013), 한국식품영양과학회
- Lauri Mikonranta JM, "Insect immunity oral exposure to a bacterial pathogen elicits free radical response and protects from a recurring infection", Front Zool, 11(23), pp.1~7 (2014)
- Martinez-Granero F, "Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas", PloS one, 9(2), pp.e87608~ (2014)[DOI]
- Miyanoshita A, "Isolation and characterization of a new member of the insect defensin family from a beetle, Allomyrina dichotoma", Biochem & Biophysic Res Comm, 220(3), pp.526~531 (1996)[DOI]
- Mulcahy H, "Drosophila melanogaster as an animal model for the study of Pseudomonas aeruginosa biofilm infections in vivo", PLoS Pathol, 7(10), pp.e1002299~ (2011)[DOI]
- Murdoch SL, "The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors", J Bacteriol, 193(21), pp.6057~6069 (2011)[DOI]
- Nunez-Valdez ME, "Identification of a putative Mexican strain of Serratia entomophila pathogenic against root-damaging larvae of Scarabaeidae (Coleoptera)", Appl & Environ Microbiol, 74(3), pp.802~810 (2008)[DOI]
- Schwarz RHaH, "Serratia marcescens internalization and replication in human bladder epithelial cells", BMC Infect Dis, 4 (2004)
- Suh HJ, "Antioxidant activity of various solvent extracts from Allomyrina dichotoma (Arthropoda:Insecta) larvae", J Photochem Photobiol B, 99(2), pp.67~73 (2010)[DOI]
- "Insect Pathology" (1994)[URL]
- White G.F, "Hornworm septicemia", J Agri Res, 26, pp.447~486 (1923)
