Development and validation of microsatellite markers for the endangered red-spotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae), in South Korea
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The red-spotted apollo butterfly, Parnassius bremeri Bremer, 1864 (Lepidoptera: Papilionidae), is an endangered species in South Korea. Development and application of molecular markers to assess population genetics perspectives can be used as a basis to establish effective conservation strategies. In this study, we developed 12 microsatellite markers specific to P. bremeri using Illumina paired-end sequencing and applied the markers to South Korean populations to understand population characteristics. Genotyping of 40 P. bremeri individuals from three localities showed that at each locus, the observed number of alleles ranged from 6 to 22 and the observed and expected heterozygosities were 0.500-1.00 and 0.465 to 0.851, respectively. Significant deviation from the Hardy–Weinberg equilibrium was not observed in all loci studied. The population based FST and RST collectively suggest that at least the Samcheok population in northernmost Gangwon Province has a significant divergence from the remaining two populations (P < 0.01), and this result is also reflected in the forewing length. Further studies with an increased sample size will be necessary to draw robust conclusions and devise conservation strategies.
Abstract
The red-spotted apollo butterfly, Parnassius bremeri Bremer, 1864 (Lepidoptera: Papilionidae), is an endangered species in South Korea. Development and application of molecular markers to assess population genetics perspectives can be used as a basis to establish effective conservation strategies. In this study, we developed 12 microsatellite markers specific to P. bremeri using Illumina paired-end sequencing and applied the markers to South Korean populations to understand population characteristics. Genotyping of 40 P. bremeri individuals from three localities showed that at each locus, the observed number of alleles ranged from 6 to 22 and the observed and expected heterozygosities were 0.500-1.00 and 0.465 to 0.851, respectively. Significant deviation from the Hardy–Weinberg equilibrium was not observed in all loci studied. The population based FST and RST collectively suggest that at least the Samcheok population in northernmost Gangwon Province has a significant divergence from the remaining two populations (P < 0.01), and this result is also reflected in the forewing length. Further studies with an increased sample size will be necessary to draw robust conclusions and devise conservation strategies.
참고문헌 (33)
- Excoffier L, "Arlequin suite ver 3. 5 : a new series of programs to perform population genetics analyses under Linux and Windows", Mol Ecol Resour, 10, pp.564~567 (2010)
- Faircloth BC, "msatcommander : detection of microsatellite repeat arrays and automated, locus-specific primer design", Mol Ecol Resour, 8, pp.92~94 (2008)
- Folmer O, "DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates", Mol Mar Biol Biotechnol, 3, pp.294~299 (1994)
- Guo SW, "Performing the exact test of Hardy-Weinberg proportions for multiple alleles", Biometrics, 48, pp.361~372 (1992)
- "FSTAT: A program to estimate and test gene diversities and fixation indices (version 2.9.3)" (2001)[URL]
- Hartl DL, "Principles of population genetics" (1997), Sinauer Associates
- Hebert PD, "Biological identifications through DNA barcodes", Proc Biol Sci, 270, pp.313~321 (2003)
- Keyghobadi N, "Influence of landscape on the population genetic structure of the alpine butterfly Parnassius smintheus(Papilionidae)", Mol Ecol, 8, pp.1481~1495 (1999)
- Kim YS, "Illustrated Book of Korean Butterflies in Color" (2005), Kyo-Hak Pub. Co.
- 김만일, "Complete Nucleotide Sequence and Organization of the Mitogenome of the Red-Spotted Apollo Butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) and Comparison with Other Lepidopteran Insects", Molecules and Cells, 28(4), pp.347~363 (2009), 한국분자세포생물학회
- Kim DS, "The factors of local disappearance and a plan of restoration for Parnassius bremeri form Okchon-gun, Korea", Korean J Environ Biol, 17, pp.469~479 (1999)
- Kim S-R, "Population genetic characterization of the Japanese oak silkmoth, Antheraea yamamai (Lepidoptera: Saturniidae), using novel microsatellite markers and mitochondrial DNA gene sequences", Genet Mol Res, 16, pp.1602908~ (2017)
- 김도성, "멸종위기종 붉은점모시나비의 서식지 복원구상", 한국조경학회지, 39(6), pp.98~109 (2011), 한국조경학회
- 김도성, "조각난 경관에서 멸종위기종 붉은점모시나비의 서식지 패치 네트워크 분석과 보전", 한국응용곤충학회지, 51(1), pp.23~31 (2012), 한국응용곤충학회
- 김도성, "멸종위기종 붉은점모시나비(Parnassius bremeri )의 메타개체군 구조와 이주", 한국응용곤충학회지, 50(2), pp.97~105 (2011), 한국응용곤충학회
- 고민수, "붉은점모시나비의 국내 분포정보 및 생태적 특성 조사", 한국응용곤충학회지, 43(1), pp.7~14 (2004), 한국응용곤충학회
- Langmead B, "Fast gapped-read alignment with Bowtie 2", Nat Methods, 9, pp.357~359 (2012)
- Larkin MA, "Clustal W and Clustal X version 2. 0", Bioinformatics, 23, pp.2947~2948 (2007)
- Meglécz E, "Comparison of differentiation pattern at allozyme and microsatellite loci in Parnassius mnemosyne(Lepidoptera)populations", Hereditas, 128, pp.95~103 (1998)
- Mira Ó, "Twenty new microsatellite loci for population structure and parentage studies of Parnassius apollo nevadensis(Lepidoptera; Papilionidae)", J Insect Conserv, 18, pp.771~779 (2014)
- Park KT, "Atlas of butterflies", Korea Research institute of bioscience and biotechnology and center for insect systematic, ~ (1997)
- Peakall R, "GenAlEx 6. 5 : genetic analysis in Excel. Population genetic software for teaching and research", Bioinformatics, 28, pp.2537~2539 (2012)
- Petenian F, "Isolation and characterization of polymorphic microsatellites in Parnassius apollo and Euphydryas aurinia (Lepidoptera)", Mol Ecol Notes, 5, pp.243~245 (2005)
- Raymond M, "GENEPOP(ver 1. 2) : population genetics software for exact tests and ecumenicism", J Hered, 86, pp.248~249 (1995)
- Rice WR, "Analyzing tables of statistical tests", Evolution, 43, pp.223~225 (1989)
- Rousset F, "genepop’007 : a complete re-implementation of the genepop software for Windows and Linux", Mol Ecol Resour, 8, pp.103~106 (2008)
- Rozen S, "Primer3 on the WWW for general users and for biologist programmers", Methods Mol Biol, 132, pp.365~386 (2000)
- Slatkin M, "A measure of population subdivision based on microsatellite allele frequencies", Genetics, 139, pp.457~462 (1995)
- Swofford DS, "PAUP* 4.0: phylogenetic analysis using parsimony (*and other methods). Version 4b2" (1999), Sinauer Associates
- Templeton AR, "Nested clade analyses of phylogeographic data : testing hypotheses about gene flow and population history", Mol Ecol, 7, pp.381~397 (1998)
- Weir SW, "Genetic data analysis II" (1990), Sinauer Associates
- Weir BS, "Estimating F-statistics for the analysis of population structure", Evolution, 38, pp.1358~1370 (1984)
- Yue GH, "Rapid isolation and characterization of microsatellites from the genome of Asian arowana(Scleropages formosus, Osteoglossidae, Pisces)", Mol Ecol, 9, pp.1007~1009 (2000)