First novel microsatellite marker sets developed for Korean Plecoptera: Development and characterization for Nemoura geei (Nemouridae) in South Korea
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Plecoptera, commonly known as stoneflies, play crucial roles in stream ecosystems, either as herbivores or predators, and are one of the key aquatic insects for assessing water quality. A total of 104 species are known from South Korea, and their weak flight ability and habitat specificity make them vulnerable, warranting studies on their population genetic features. However, population-level analyses of genetic diversity and structure using polymorphic microsatellite markers have not been conducted in any of these species. Here, we first developed and characterized novel microsatellite markers for Nemoura geei, a dominant stonefly species in Korea, to investigate its genetic diversity and population structure. Using high-throughput sequencing, we developed the first set of microsatellite markers for a Korean Plecoptera species. The markers revealed low genetic diversity across three populations, suggesting small effective population sizes and high conservation risks. These novel microsatellite markers will be highly useful for understanding the population genetics and conservation status of N. geei from broader distributions in Asia.
Abstract
Plecoptera, commonly known as stoneflies, play crucial roles in stream ecosystems, either as herbivores or predators, and are one of the key aquatic insects for assessing water quality. A total of 104 species are known from South Korea, and their weak flight ability and habitat specificity make them vulnerable, warranting studies on their population genetic features. However, population-level analyses of genetic diversity and structure using polymorphic microsatellite markers have not been conducted in any of these species. Here, we first developed and characterized novel microsatellite markers for Nemoura geei, a dominant stonefly species in Korea, to investigate its genetic diversity and population structure. Using high-throughput sequencing, we developed the first set of microsatellite markers for a Korean Plecoptera species. The markers revealed low genetic diversity across three populations, suggesting small effective population sizes and high conservation risks. These novel microsatellite markers will be highly useful for understanding the population genetics and conservation status of N. geei from broader distributions in Asia.