Effects of Oxya chinensis sinuosa hot water extract on benign prostatic hyperplasia in LNCaP cells
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
In recent years, the number of patients with benign prostatic hyperplasia (BPH), a condition that commonly occurs in elderly men, has increased due to aging and the adoption of western dietary habits. Treatment with chemical drugs, such as finasteride or dutasteride, can cause side effects such as erectile dysfunction or sexual problems. This necessitates the development of remedies using natural substances derived from food ingredients. In this study, we investigated the inhibitory effects of Oxya chinensis sinuosa hot water extract (OCH) on BPH production in LNCaP cells, a hormone-dependent prostate cancer cell line. We found that the mRNA expression of androgen receptor (AR), prostate specific antigen (PSA), and, 5α-reductases 1, and 2 decreased following treatment with OCH. Furthermore, OCH treatment resulted in reduced protein expression of BPH regulators, such as AR. Collectively, these results suggest that OCH exerts a beneficial effect on BPH by inhibiting the AR signaling pathway, indicating the potential of OCH as a therapeutic agent for the prevention and treatment of BPH.
Abstract
In recent years, the number of patients with benign prostatic hyperplasia (BPH), a condition that commonly occurs in elderly men, has increased due to aging and the adoption of western dietary habits. Treatment with chemical drugs, such as finasteride or dutasteride, can cause side effects such as erectile dysfunction or sexual problems. This necessitates the development of remedies using natural substances derived from food ingredients. In this study, we investigated the inhibitory effects of Oxya chinensis sinuosa hot water extract (OCH) on BPH production in LNCaP cells, a hormone-dependent prostate cancer cell line. We found that the mRNA expression of androgen receptor (AR), prostate specific antigen (PSA), and, 5α-reductases 1, and 2 decreased following treatment with OCH. Furthermore, OCH treatment resulted in reduced protein expression of BPH regulators, such as AR. Collectively, these results suggest that OCH exerts a beneficial effect on BPH by inhibiting the AR signaling pathway, indicating the potential of OCH as a therapeutic agent for the prevention and treatment of BPH.
참고문헌 (35)
- 박지아, "테스토스테론-유도 양성전립선비대증에서 나노화 벌 화분의 효능 연구", 생명과학회지, 28(4), pp.465~471 (2018), 한국생명과학회[DOI]
- Banerjee PP, "Androgen action in prostate function and disease", Am J Clin Exp Urol, 6, pp.62~77 (2018)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 29, pp.1199~1200 (1958)
- Bostanci Y, "Correlation between benign prostatic hyperplasia and inflammation", Curr Opin Urol, 23, pp.5~10 (2013)[DOI]
- Branen AS, "Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene", J Am Oil Chem Soc, 52, pp.59~63 (1975)[DOI]
- Buszewska-Forajta M, "Determination of water-soluble components of abdominal secretion of grasshopper(Chorthippus SPP. )by GC", MS, Chromatographia, pp.2014, 77[DOI]
- Da Silva MHA, "Current evidence for the involvement of sex steroid receptors and sex hormones in benign prostatic hyperplasia", Res Rep Urol, 11, pp.1~8 (2019)[DOI]
- Dhurat R, "5-alpha reductase inhibitors in androgenetic alopecia:shifting paradigms, current concepts, comparative efficacy, and safety", Dermatol Ther, 33, pp.e13379~ (2020)[DOI]
- 한상미, "무당벌레(Harmonia axyridis) 추출물에 의한 BV-2 세포주의 Nitric Oxide 생성 저해 활성", 한국응용곤충학회지, 45(1), pp.31~36 (2006), 한국응용곤충학회
- Im AR, "Protective effects of Oxya chinensis sinuosa Mishchenko against ultraviolet B-induced photodamage in hairless mice", BMC Complement Altern Med, 19, pp.286~ (2019)[DOI]
- 오석중, "토마토 추출액 복합체가 전립선 암 세포와 전립선 비대증에 미치는 영향", 생약학회지, 38(2), pp.197~203 (2007), 한국생약학회
- 강성림, "고콜레스테롤 식이 섭취 흰쥐에 대한 적송잎 추출물의 항산화 효과", 한국식품영양과학회지, 38(4), pp.423~429 (2009), 한국식품영양과학회
- 김은경, "버섯류 추출물이 전립선 암 세포 내 PSA 발현에 미치는 영향", 한국버섯학회지, 13(3), pp.181~184 (2015), 한국버섯학회
- Kim HJ, "Antioxidant activity and antimicrobial activity of the grasshopper, Oxya chinenesis sinuosa", J Seric Entomol Sci, 53, pp.130~134 (2015)[DOI]
- 김인우, "Transcriptome Profiling and In Silico Analysis of the Antimicrobial Peptides of the Grasshopper Oxya chinensis sinuosa", Journal of Microbiology and Biotechnology, 26(11), pp.1863~1870 (2016), 한국미생물·생명공학회
- 김민영, "산수유 추출물에 의한 LNCaP 전립선 세포의 증식 억제 및 양성 전립선 비대증 유발 인자의 발현에 미치는 영향", 한방비만학회지, 21(1), pp.10~21 (2021), 한방비만학회[DOI]
- Kim TS, "Nutritional value of dried paddy grasshopper, Oxya chinensis formosana", J Korean Soc Food Nutr, 16, pp.98~104 (1987)
- 김이슬, "벼메뚜기 섭취와 트레드밀 운동 수행에 대한혈중 지질 및 항산화 효과", 한국체육과학회지, 27(5), pp.1605~1614 (2018), 한국체육과학회[DOI]
- Lee JM, "Development of a method predicting the oxidative stability of edible oils using 2, 2-diphenyl-1-picrylhydrazyl(DPPH)", Food Chem, 103, pp.662~669 (2007)[DOI]
- 이수정, "복분자 미숙과 추출물이 전립선암 세포주와 전립선비대 백서모델에 미치는 영향", 한국식품영양과학회지, 43(4), pp.507~515 (2014), 한국식품영양과학회
- Liu J, "Current state of biomarkers of the diagnosis and assessment of treatment efficacy of prostate cancer", Discov Med, 27, pp.235~243 (2019)
- Madersbacher S, "Pathophysiology of benign prostatic hyperplasia and benign prostatic enlargement : a mini-review", Gerontology, 65, pp.1~7 (2019)[DOI]
- Minciullo PL, "Oxidative stress in benign prostatic hyperplasia : a systematic review", Urol Int, 94, pp.249~254 (2015)[DOI]
- Nixon P, "New clinical trial of medical therapy for benign prostatic hyperplasia", Drug Benefit Trends, 9, pp.44~45 (1997)
- Park E, "Paljung-San, a traditional herbal medicine, attenuates benign prostatic hyperplasia in vitro and in vivo", J Ethnopharmacol, 218, pp.109~115 (2018)[DOI]
- 박재은, "Oxya chinensis sinuosa Mishchenko (Grasshopper) Extract Protects INS-1 Pancreatic β cells against Glucotoxicity-induced Apoptosis and Oxidative Stress", 생명과학회지, 31(11), pp.969~979 (2021), 한국생명과학회[DOI]
- 박자영, "약용곤충추출물 라이브러리를 이용한 항산화 활성의 초고속 검색", Food Science and Preservation, 12(5), pp.482~488 (2005), 한국식품저장유통학회
- 박자영, "메뚜기류 추출물의 염증 조절작용 및 세포사멸보호 효과", Food Science and Preservation, 13(6), pp.796~802 (2006), 한국식품저장유통학회
- Richard A, "Structure-activity relationships for inhibition of human 5α-reductase by polyphenols", Biochem Pharmacol, 63, pp.1165~1176 (2002)[DOI]
- Thomas D, "Emerging drug therapies for benign prostatic hyperplasia", Expert Opin Emerg Drugs, 22, pp.201~212 (2017)[DOI]
- Traish AM, "Adverse side effects of 5alpha-reductase inhibitors therapy : persistent diminished libido and erectile dysfunction and depression in a subset of patients", J Sex Med, 8, pp.872~884 (2011)[DOI]
- Velonas VM, "Current status of biomarkers for prostate cancer", Int J Mol Sci, 14, pp.11034~11060 (2013)[DOI]
- Vickman RE, "The role of the androgen receptor in prostate development and benign prostatic hyperplasia : a review", Asian J Urol, 7, pp.191~202 (2020)[DOI]
- Whang HJ, "Quantitative analysis of total phenolic content in apple", Anal Sci Technol, 14, pp.377~383 (2001)
- 윤영일, "LPS로 유도된 RAW 264.7세포에 대한 벼메뚜기(Oxya chinensis sinuosa) 에탄올 추출물의 항염증 효과", 생명과학회지, 24(4), pp.370~376 (2014), 한국생명과학회