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Gene-expression profiling of titanium-cell interaction

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Statement of problem. In the process of bone formation, titanium (Ti) surface roughness is an important factor modulating osteoblastic function. Purpose. This study was carried out to determine the effect of different Ti surface on biologic responses of a human osteoblast-like cell line (MG63). Materials and methods. MG63 cells were cultured on S (smooth), SLA (sandblasted largegrit & acid etching), HA (hydroxyapatite) Ti. The morphology and attachment of the cells were examined by SEM. The cDNAs prepared from total RNAs of MG63 were hybridized to a human cDNA microarray (1,152 elements). Results. The appearances of the surfaces observed with SEM were different in the three types of dental substrates. The surface of SLA and HA were shown to be rougher than S. MG63 cells cultured on SLA and HA were cell-matrix interaction. In the expression of genes involved in osseointegration, upregulated genes were bone morphogenetic protein, Villin, Integrin, Insulin-like growth factors in different surfaces. Downregulated genes were fibroblast growth factor receptor 4, Bcl 2-related protein, collagen, CD4 in different surfaces. Conclusion. The attachment and expression of key osteogenic regulatory genes were enhanced by surface roughness of the dental materials.

Abstract

Statement of problem. In the process of bone formation, titanium (Ti) surface roughness is an important factor modulating osteoblastic function. Purpose. This study was carried out to determine the effect of different Ti surface on biologic responses of a human osteoblast-like cell line (MG63). Materials and methods. MG63 cells were cultured on S (smooth), SLA (sandblasted largegrit & acid etching), HA (hydroxyapatite) Ti. The morphology and attachment of the cells were examined by SEM. The cDNAs prepared from total RNAs of MG63 were hybridized to a human cDNA microarray (1,152 elements). Results. The appearances of the surfaces observed with SEM were different in the three types of dental substrates. The surface of SLA and HA were shown to be rougher than S. MG63 cells cultured on SLA and HA were cell-matrix interaction. In the expression of genes involved in osseointegration, upregulated genes were bone morphogenetic protein, Villin, Integrin, Insulin-like growth factors in different surfaces. Downregulated genes were fibroblast growth factor receptor 4, Bcl 2-related protein, collagen, CD4 in different surfaces. Conclusion. The attachment and expression of key osteogenic regulatory genes were enhanced by surface roughness of the dental materials.

참고문헌 (49)

  1. "Interface shear strengthof titanium implants with a sandblasted andacidetched surface:a biomechanical study in themaxilla of miniature pigs", 45, pp.75~83 (1999)
  2. "Removal torque and histomorphometric investi-gation of 4 different titanium surfaces:an experi-mental study in the rabbit tibia", 15, pp.668~74 (2000)
  3. "Anchorageof titanium implants with different surface char-acteristics:an experimental study in rabbits", 2, pp.120~9 (2000)
  4. "Surface rough-ness modulates the response of MG 63 osteoblast-like cells to 1 Journal of Biomedical MaterialsResearch 1999", 37, pp.139~51 (1999)
  5. "Histologic comparison of a thermaldual-etched implant surface to machined,TPS,and HA surfaces:bone contact in vivo in rabbits", 17, pp.369~376 (2002)
  6. "Titanium-cell interaction:analysis of geneexpression profiling", 66b, pp.341~6 (2003)
  7. "High-res-olution morphometric analysis of human os-teoblastic cell adhesion on clinically relevant or-thopedic alloys", 24, pp.499~506 (1999)
  8. "Modulatedbone matrix-related gene expression is associatedwith differences in interfacial strength of differentimplant surface roughness", 11, pp.241~7 (2002)
  9. "Implantsurface roughness affects osteoblast gene expres-sion", 82, pp.372~6 (2003)
  10. "Microrough implant surface topographies in-crease osteogenesis by reducing osteoclast for-mation and activity", 70a, pp.361~9 (2004)
  11. "Zirconium oxide:analysisof MG63 osteoblast-like cell response by means ofa microarray technology", 25, pp.215~28 (2004)
  12. "Surface analysis of machined versus sand-blasted and acid-etched titanium implants", 15, pp.779~84 (2000)
  13. "Analysisof MG63 osteoblastic-cell response to a newnanoporous implant surface by means of a mi-croarray technology", 15, pp.180~6 (2004)
  14. "Analysis ofosteoblast-like MG63 cells’response to a rough im-plant surface by means of DNA microarray", 29, pp.215~20 (2003)
  15. "Calciumsulfate:analysis of MG63 osteoblast-like cell responseby means of a microarray technology", 71b, pp.260~7 (2004)
  16. "Determining optimal sur-face roughness of TiO blasted titanium im-plant material for attachment proliferation and dif-ferentiation of cells derived from human mandibu-lar alveolar bone", 12, pp.515~25 (2001)
  17. "Different bone integra-tion profiles of turned and acid-etched implants as-sociated with modulated expression of extracellularmatrix genes", 18, pp.200~10 (2003)
  18. "Application of cDNA microar-rays to examine gene expression differences inschizophrenia", 55, pp.641~50 (2001)
  19. "Use of acDNA microarray to analyse gene expression pat-terns in human cancer", 14, pp.457~60 (1996)
  20. "Genome-wide expression profiling of 8-chloroadenosine- and 8-chloro-cAMP-treated hu-man neuroblastoma cells using radioactive hu-man cDNA microarray", 34, pp.184~93 (2002)
  21. "Genome-wide expression profiling of mid-ges-tation placenta and embryo using a 15 Proceeding ofthe National Academy of Science of the UnitedStates of America 2000", 97, pp.9127~32 (2000)
  22. "Cluster analysis and display of genome-wide ex-pression patterns", 95, pp.14863~8 (1998)
  23. "Differential expression of osteogenic factors as-sociated with osteoinductivity of human os-teosarcoma cell lines", 70a, pp.122~8 (2004)
  24. "In vivo convergence ofBMP and MAPK signaling pathways:impact of dif-ferential Smad1 phosphorylation on developmentand homeostasis", 18, pp.1482~94 (2004)
  25. "Bone morphogenetic protein 2 incorpo-rated into biomimetic coatings retains its biolog-ical activity", 10, pp.101~8 (2004)
  26. "Expressionof bone sialoprotein in mineralized tissues oftooth and bone and in buccal-pouch carcinomas ofSyrian golden hamsters", 45, pp.551~62 (2000)
  27. "Expression of bone morpho-genetic protein in the course of osteoinductionby recombinant human bone morphogenetic pro-tein-2", 13, pp.80~5 (2002)
  28. "Early gene expres-sion analyzed by cDNA microarray and RT-PCRin osteoblasts cultured with water-soluble andlow molecular chitooligosaccharide", 25, pp.1749~54 (2004)
  29. "Regulation ofNotch signaling genes during BMP2-induced dif-ferentiation of osteoblast precursor cells", 320, pp.100~7 (2004)
  30. "Bone morphogenetic proteins:an un-conventional approach to isolation of first mam-malian morphogens", 8, pp.11~20 (1997)
  31. "BMP stimulation of bone response adjacent to ti-tanium implants in vivo", 10, pp.212~8 (1999)
  32. "Differential expression of Xenopus BMPs in earlyembryos and tissues", pp.175~8 (1993)
  33. "Purificationand characterization of other distinct bone-in-ducing factors", 85, pp.9484~8 (1988)
  34. "Novel regulators of bone formation:molecularclones and activities", 242, pp.1528~34 (1988)
  35. "Involvementof Bone Morphogenetic Protein-4(BMP-4)andVgr-1 in morphogenesis and neurogenesis in themouse", 111, pp.531~42 (1991)
  36. "Distinct and overlapping patterns of localizationof bone morphogenetic protein(BMP)familymembers and a BMP type II receptor during frac-ture healing in rats", 22, pp.605~12 (1998)
  37. "Regulation of insulin-like growth factor-II synthesis in bone cell cul-tures by skeletal growth factors", 135, pp.284~9 (1994)
  38. "Intact but not truncated insulin-like growthfactor binding protein-3 blocks IGF I-in-duced stimulation of osteoblasts control of IGF sig-407nalling to bone cells by IGFBP-3-specific proteol-ysis", pp.579~3 85 (1991)
  39. "Regulation of insulin-likegrowth factor system components by osteogenic pro-tein-1 in human bone cells", 136, pp.857~65 (1995)
  40. "Bone morphogenetic protein2 increases insulin-like growth factor I and IItranscripts and polypeptide levels in bone cellcultures", 9, pp.1999~2005 (1994)
  41. "The TGF-beta superfamily:newmembers,new receptors,and new genetic tests offunction in different organisms", 8, pp.133~46 (1994)
  42. "Insulin-like growth factors", 19, pp.445~57 (1995)
  43. "A role forthe insulin-like growth factor II", mannose-6-phos-phate receptor in the insulin-induced inhibition ofprotein catabolism, 1989, pp.3, 901~6
  44. "Large-scale gene expression analysis of os-teoblasts cultured on three different Ti-6Al-4Vsurface treatments", 23, pp.4193~202 (2002)
  45. "Gene expression analysis of osteoblasticcells contacted by orthopedic implant particles", 61, pp.408~20 (2002)
  46. "Inhibition of alpha and tumorcell proliferation in experimental prostate cancerbone metastases", pp.413~20 (2003)
  47. "Engagement of alphavbeta3 integrinregulates proliferation and apoptosis of hepatic stel-late cells Journal of Biology & Chemistry2004", pp.23996~4006 (2004)
  48. "promotes cell adhesion of humanastrocytoma cells in vitro implication of alpha6-beta4 integrin", pp.93~6 (2003)
  49. "Ligation of the beta4 inte-grin triggers adhesion behavior of human ker-atinocytes by an", pp.444~51 (2004)

관련 논문AI추천

Effect of implant surface microtopography by hydroxyapatite grit-blasting on adhesion, proliferation, and differentiation of osteoblast-like cell line, MG-63김수경, 박성재, 배상범, 송승일, 엄태관대한구강악안면외과학회 · 대한구강악안면외과학회지2011 · 제37권 제3호DNA microarray analysis of gene expression of MC3T3-E1 osteoblast cell cultured on anodized- or machined titanium surface강나라, 김명래, 박주미, 방은경, 전혜란대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제2호Gene expression of MC3T3-E1 osteoblastic cells on titanium and zirconia surface공순현, 노관태, 배아란, 신용문, 우이형, 이정행, 이희수대한치과보철학회 · The Journal of Advanced of Prosthodontics2013 · 제5권 제4호Implant surface treatments affect gene expression of Runx2, osteogenic key marker나영, 곽재영, 김성균, 허성주대한치과보철학회 · The Journal of Advanced of Prosthodontics2009 · 제1권 제2호Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2구영, 구기태, 김경화, 김태일, 류인철, 설양조, 이용무, 한승희, 한중석대한치주과학회 · Journal of Periodontal & Implant Science2011 · 제41권 제5호Cellular attachment and gene expression of osteoblast-like cells on zirconia ceramic surfaces백진, 김형섭, 배아란, 우이형, 이희수대한치과보철학회 · 대한치과보철학회지2008 · 제46권 제3호

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