마우스 모델에서 Lactobacillus acidophilus의 천식 발생에 대한 일차 예방 효과
The Effect of Lactobacillus acidophilus on the Primary Prevention of Asthma in a Murine Asthmatic Model
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Purpose : Recent studies have demonstrated that probiotics are effective in the management of allergic diseases, but there have been few reports about their preventive effects in asthma. We examined the effects of Lactobacillus acidophilus (LA) and its mechanism in the primary prevention of asthma. Methods : Before allergen sensitization, newborn Balb/c mice orally received 1×109 colony forming unit (CFU) LA per day every 2 days for consecutive 4 weeks starting from birth. After ovalbumin sensitization and challenge from day 36, airway hyperresponsiveness (AHR) to methacholine, influx of inflammatory cells to the lung, serum total IgE levels, cytokine levels in the supernatant of splenocytes culture, and expression of FoxP3 mRNA in the peribronchial lymph nodes were assessed. Results : The AHR showed a tendency to decrease in LA group, however, there was no statistical significance. The eosinophil counts in bronchoalveolar lavage fluid and serum total IgE were significantly reduced in LA group compared with asthma control group. LA effectively induced IFN-γ secretion and inhibited IL-4, IL-5 and TGF-β1 secretion compared with asthma control group. The expression of FoxP3 mRNA in peribronchial lymph nodes did not show any difference between LA and asthma control group. Conclusion : Oral LA administration in murine model of asthma attenuated AHR and significantly suppressed eosinophilic inflammation in the airway. These effects of LA were mediated by suppressing Th2 immune response and enhancing Th1 immune response. The results may suggest the possible role of LA in the primary prevention of asthma.
Abstract
Purpose : Recent studies have demonstrated that probiotics are effective in the management of allergic diseases, but there have been few reports about their preventive effects in asthma. We examined the effects of Lactobacillus acidophilus (LA) and its mechanism in the primary prevention of asthma. Methods : Before allergen sensitization, newborn Balb/c mice orally received 1×109 colony forming unit (CFU) LA per day every 2 days for consecutive 4 weeks starting from birth. After ovalbumin sensitization and challenge from day 36, airway hyperresponsiveness (AHR) to methacholine, influx of inflammatory cells to the lung, serum total IgE levels, cytokine levels in the supernatant of splenocytes culture, and expression of FoxP3 mRNA in the peribronchial lymph nodes were assessed. Results : The AHR showed a tendency to decrease in LA group, however, there was no statistical significance. The eosinophil counts in bronchoalveolar lavage fluid and serum total IgE were significantly reduced in LA group compared with asthma control group. LA effectively induced IFN-γ secretion and inhibited IL-4, IL-5 and TGF-β1 secretion compared with asthma control group. The expression of FoxP3 mRNA in peribronchial lymph nodes did not show any difference between LA and asthma control group. Conclusion : Oral LA administration in murine model of asthma attenuated AHR and significantly suppressed eosinophilic inflammation in the airway. These effects of LA were mediated by suppressing Th2 immune response and enhancing Th1 immune response. The results may suggest the possible role of LA in the primary prevention of asthma.
참고문헌 (39)
- Garn H, "Epidemiological and immunological evidence for the hygiene hypothesis", Immunobiology, 212, pp.441~452 (2007)
- Strachan DP, "Hay fever, hygiene and household size", BMJ, 299, pp.1259~1260 (1989)
- Braun-Fahrlander C, "Prevalence of hay fever and allergic sensitization in farmer's children and their peers living in the same rural community. SCARPOL team. Swiss Study on Childhood Allergy and Respiratory Symptoms with Respect to Air Pollution", Clin Exp Allergy, 29, pp.28~34 (1999)
- Sepp E, "Intestinal microflora of Estonian and Swedish infants", Acta Paediatr, 86, pp.956~961 (1997)
- Björkstén B, "The intestinal microflora in allergic Estonian and Swedish 2-year-old children", Clin Exp Allergy, 29, pp.342~346 (1999)
- Viljanen M, "Probiotics in the treatment of atopic eczema", dermatitis syndrome in infants: a double- blind placebo-controlled trial, 2005, pp.60, 494~500
- Weston S, "Effects of probiotics on atopic dermatitis: a randomized controlled trial", Arch Dis Child, 90, pp.892~897 (2005)
- Brouwer ML, "No effects of probiotics on atopic dermatitis in infancy: a randomized placebo-controlled trial", Clin Exp Allergy, 36, pp.899~906 (2006)
- Lee J, "Meta-analysis of clinical trials of probiotics for prevention and treatment of pediatric atopic dermatitis", J Allergy Clin Imunol, 121, pp.116~121 (2008)
- Helin T, "No effect of oral treatment with an intestinal bacterial strain, Lactobacillus rhamnosus (ATCC 53103), on birch-pollen allergy: a placebo-controlled double-blind study", Allergy, 57, pp.243~246 (2002)
- Wang MF, "Treatment of perennial allergic rhinitis with lactic acid bacteria", Pediatr Allergy Immunol, 15, pp.152~158 (2004)
- Ishida Y, "Clinical effects of Lactobacillus acidophilus strain L- 92 on perennial allergic rhinitis: a double- blind, placebo-controlled study", J Dairy Sci, 88, pp.527~533 (2005)
- Wheeler JG, "Immune and clinical impact of Lactobacillus acidophilus on asthma", Ann Allergy Asthma Immunol, 79, pp.229~233 (1997)
- Ginovannini M, "Felicita Study Group. A randomized prospective double blind controlled trial on effects of long- term consumption of fermented milk containing Lactobacillus casei in pre-school children with allergic asthma and", or rhinitis, 2007, pp.62, 215~220
- Zhou JS, "Acute oral toxicity and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria", Food Chem Toxicol, 38, pp.153~161 (2000)
- Cebra JJ, "Influences of microbiota on intestinal immune system development", Am J Clin Nurt, 69, pp.1046S~1051S (1999)
- Prescott SL, "Early origins of allergic disease: a review of processes and influences during early immune development", Curr Opin Allergy Clin Immunol, 3, pp.125~132 (2003)
- Lemberg DA, "Probiotics in paediatric gastrointestinal diseases", J Paediatr Child Healthy, 43, pp.331~336 (2007)
- Feleszko W, "Probiotic-induced suppression of allergic sensitization and airway inflammation is associated with an increase of T regulatory-dependent mechanisms in a murine model of asthma", Clin Exp Allergy, 37, pp.498~505 (2007)
- Forsythe P, "Oral treatment with live Lactobacillus reuteri inhibits the allergic airway response in mice", Am J Respir Crit Care Med, 175, pp.561~569 (2007)
- Christensen HR, "Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells", J Immunol, 168, pp.171~178 (2002)
- Mohamadzadeh M, "Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization", Proc Natl Acad Sci USA, 통권 : 102, pp.2880~2885 (2005)
- Ezendam J, "Probiotics: immunomodulation and evaluation of safety and efficacy", Nutr Rev, 64, pp.1~14 (2006)
- Cross ML, "Dietary intake of Lactobacillus rhamnosus HN001 enhances production of both Th1 and Th2 cytokines in antigen-primed mice", Med Microbiol Immunol (Berl), 191, pp.49~53 (2002)
- Aldinucci C, "Effects of dietary yoghurt on immunological and clinical parameters of rhinopathic patients", Eur J Clin Nutr, 56, pp.1155~1561 (2002)
- Pohjavuori E, "Lactobacillus GG effect in increasing IFN-gamma production in infants with cow's milk allergy", J Allergy Clin Immunol, 114, pp.131~136 (2004)
- Akdis M, "Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells", J Exp Med, 199, pp.1567~1575 (2004)
- Ling EM, "Relation of CD4＋CD25＋ regulatory T-cell suppression of allergen-driven T cell activation to atopic status and expression of allergic disease", Lancet, 363, pp.608~615 (2004)
- Smits HH, "Selective probiotic bacteria induce IL-10-producing regulatory T cells in vitro by modulating dendritic probiotic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin", J Allergy Clin Immunol, 115, pp.1260~1267 (2005)
- Di Giacinto C, "Probiotics ameliorate recurrent Th1-mediated murine colitis by inducing IL-10 and IL-10-dependent TGF-beta- bearing regulatory cells", J Immunol, 74, pp.3237~3246 (2005)
- Braat H, "Lactobacillus rhamnosus induces peripheral hyporesponsiveness in stimulated CD4＋ T cells via modulation of dendritic cell function", Am J Clin Nutr, 80, pp.1618~1625 (2004)
- Adams MR, "On the safety of lactic acid bacteria", Int J Food Micro, 27, pp.263~264 (1995)
- Saavedra JM, "Long-term consumption of infant formulas containing live probiotic bacteria: tolerance and safety", Am J Clin Nutr, 79, pp.261~267 (2004)
- Deshpande G, "Probiotics for prevention of necrotising enterocolitis in preterm neonates with very low birth weight: a systematic review of randomised controlled trials", Lancet, 369, pp.1614~1620 (2007)
- Marteau P, "Tolerance of probiotics and prebiotics", J Clin Gastroenterol, 38(6), pp.67~69 (2004)
- Boyle RJ, "The role of probiotics in the management of allergic disease", Clin Exp Allergy, 36, pp.568~576 (2006)
- Penders J, "The role of the intestinal microbiota in the development of atopic disorders", Allergy, 11, pp.1223~1236 (2007)
- Bullens DM, "IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx?", Respir Res, 7, pp.135~ (2006)
- Nassenstein C, "Neuroimmune crosstalk in asthma: dual role of the neurotrophin receptor p75NTR", J Allergy Clin Immunol, 120, pp.1089~1096 (2007)