Production of Interleukin-5, Interleukin-13 and Interferon-γ in Peripheral Blood CD8＋ T Cells from Children with Wheezing
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Purpose : Our objective was to investigate the role of CD8＋ T cells in pathogenesis of wheezing in children with atopic nature. Methods : Twelve atopic wheezers, 8 nonatopic wheezers, 8 disease controls and 8 healthy controls were enrolled in the study. We isolated CD8＋ T cells from peripheral blood samples, incubated them for 72 hours either in the absence or presence of phytohemagglutinin (PHA) and compared the concentrations of interleukin (IL)-5, IL-13, and interferon (IFN)-γ in the cell culture supernatants. Results : In the atopic wheezer group, the IL-5 concentration was significantly higher after PHA stimulation than after non-stimulated incubation. This difference was not observed in the nonatopic wheezer, disease control and healthy control groups. IL-13 was undetectable in all of the cell culture supernatants. There was no significant difference in the IFN-γ concentration between the PHA-stimulated and non-stimulated conditions in all 4 groups. Conclusion : The results of this study suggest that CD8＋ T cells may play a role in the pathogenesis of wheezing in children with atopic nature through the production of IL-5.
Abstract
Purpose : Our objective was to investigate the role of CD8＋ T cells in pathogenesis of wheezing in children with atopic nature. Methods : Twelve atopic wheezers, 8 nonatopic wheezers, 8 disease controls and 8 healthy controls were enrolled in the study. We isolated CD8＋ T cells from peripheral blood samples, incubated them for 72 hours either in the absence or presence of phytohemagglutinin (PHA) and compared the concentrations of interleukin (IL)-5, IL-13, and interferon (IFN)-γ in the cell culture supernatants. Results : In the atopic wheezer group, the IL-5 concentration was significantly higher after PHA stimulation than after non-stimulated incubation. This difference was not observed in the nonatopic wheezer, disease control and healthy control groups. IL-13 was undetectable in all of the cell culture supernatants. There was no significant difference in the IFN-γ concentration between the PHA-stimulated and non-stimulated conditions in all 4 groups. Conclusion : The results of this study suggest that CD8＋ T cells may play a role in the pathogenesis of wheezing in children with atopic nature through the production of IL-5.
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