천식 마우스 모델에서 BCG백신의 투여시기에 따른 알레르기 염증반응 억제효과
Effects of Bacillus Calmette-Guerin (BCG) Vaccination according to Time Point of Administration on Asthma in a Murine Model
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Background: Asthma is a chronic allergic disease characterized by the infiltration of eosinophils into the airway and lung. Bacille Calmette-Guerin (BCG) has been reported to be beneficial to inhibit allergic inflammation in an animal asthma model. Objective: The aims of the study were to investigate the efficacy of BCG according to the time point of administration on asthma in a murine model. Method: BALB/c mice were treated with BCG (1×106CFUs) before sensitization (−5 days) or after sensitization (＋12 days) in asthma-induced BALB/c mice by ovalbumin (OVA) sensitization. Bronchial hyperresponsiveness (BHR),influx of inflammatory cells in bronchoalveolar lavage (BAL) fluid, histopathological changes in the lung and systemic immune responses were assessed. Result: The administration of BCG before and after sensitization suppressed asthmatic responses, including BHR,OVA-specific immunoglobulin E (IgE) production, percentage of eosinophils in BALF and pulmonary eosinophilic inflammation in the murine model compared to the control group. However, total IgE, total cell count and OVA-IgG1were significantly suppressed by BCG before sensitization only. Conclusion: BCG vaccinations, regardless of administration time point, suppressed asthmatic responses in mice. The mechanism of effect of BCG before sensitization is thought to be balance Th1/Th2 immune responses, but, that of BCG after sensitization may be different from BCG before sensitization.
Abstract
Background: Asthma is a chronic allergic disease characterized by the infiltration of eosinophils into the airway and lung. Bacille Calmette-Guerin (BCG) has been reported to be beneficial to inhibit allergic inflammation in an animal asthma model. Objective: The aims of the study were to investigate the efficacy of BCG according to the time point of administration on asthma in a murine model. Method: BALB/c mice were treated with BCG (1×106CFUs) before sensitization (−5 days) or after sensitization (＋12 days) in asthma-induced BALB/c mice by ovalbumin (OVA) sensitization. Bronchial hyperresponsiveness (BHR),influx of inflammatory cells in bronchoalveolar lavage (BAL) fluid, histopathological changes in the lung and systemic immune responses were assessed. Result: The administration of BCG before and after sensitization suppressed asthmatic responses, including BHR,OVA-specific immunoglobulin E (IgE) production, percentage of eosinophils in BALF and pulmonary eosinophilic inflammation in the murine model compared to the control group. However, total IgE, total cell count and OVA-IgG1were significantly suppressed by BCG before sensitization only. Conclusion: BCG vaccinations, regardless of administration time point, suppressed asthmatic responses in mice. The mechanism of effect of BCG before sensitization is thought to be balance Th1/Th2 immune responses, but, that of BCG after sensitization may be different from BCG before sensitization.
참고문헌 (22)
- Walker C, "Allergic and nonallergic asthmatics have distinct patterns of T-cell activation and cytokine production in peripheral blood and bronchoalveolar lavage", Am Rev Resp Dis, 146, pp.109~15 (1992)
- Wang JM, "Sanderson CJ, Montovani A. Recombinant human interleukin-5 is a selective eosinophil chemoattractant", Eur J Immunol, 19, pp.701~5 (1989)
- Eder W, "The asthma epidemic", N Engl J Med, 355, pp.2226~35 (2006)
- 홍수종, "한국 소아알레르기 질환의 유병률", Korean Journal of Pediatrics, 51(4), pp.343~350 (2008), 대한소아과학회
- 이상일, "Prevalence of Childhood Asthma in Korea: International Study of Asthma and Allergies in Childhood", Allergy, Asthma & Immunology Research, 2(2), pp.61~64 (2010), 대한천식알레르기학회
- Strachan DP, "Hay fever, hygiene and household size", Br Med J, 299, pp.1259~60 (1989)
- Riedler J, "Exposure to farming in early life and development of asthma and allergy: a cross-sectional survey", Lancet, 358, pp.1129~33 (2001)
- Shirakawa T, "The inverse association between tuberculin responses and atopic disorder", Science, 275, pp.77~9 (1996)
- Hopfenspirger MT, "Airway hyperresponsiveness, late allergic response, and eosinophilia are reversed with mycobacterial antigens in ovalbumin-presensitized mice", J Immunol, 168, pp.2516~22 (2002)
- Oliveira FH, "Production of interferon gamma in asthmatic patients with small bacille Calmette-Guerin scars: a pilot study", Allergy Asthma Proc, 27, pp.516~22 (2006)
- Coffman RL, "The role of helper T cell products in mouse B cell differentiation and isotype regulation", Immunol Rev, 102, pp.5~28 (1988)
- Yang X, "Mycobacterial infection inhibits established allergic inflammatory responses via alteration of cytokine production and vascular cell adhesion molecule-1 expression", Immunology, 105, pp.336~43 (2002)
- Nahori MA, "Effects of Mycobacterium bovis BCG on the development of allergic inflammation and bronchial hyperresponsiveness in hyper-IgE BP2 mice vaccinated as newborns", Vaccine, 19, pp.1484~95 (2001)
- Shen H, "Neonatal vaccination with Bacillus Calmette-Guerin elicits longterm protection in mouse-allergic responses", Allergy, 63, pp.555~63 (2008)
- Riffo-Vasquez Y, "Effect of Mycobacterium tuberculosis chaperonins on bronchial eosinophilia and hyper-responsiveness in a murine model of allergic inflammation", Clin Exp Allergy, 34, pp.712~9 (2004)
- Tournoy KG, "Airway eosinophilia is not a requirement for allergen-induced airway hyperresponsiveness", Clin Exp Allergy, 30, pp.79~85 (2000)
- Erb KJ, "Infection of mice with Mycobacterium bovis-BCG suppresses allergeninduced airway eosinophilia", J Exp Med, 187, pp.561~9 (1998)
- Herz U, "BCG infection suppresses allergic sensitization and development of increased airway reactivity in an animal model", J Allergy Clin Immunol, 102, pp.867~74 (1998)
- Ahrens B, "BCG priming of dendritic cells enhances T regulatory and Th1 function and suppresses allergen-induced Th2 function in vitro and in vivo", Int Arch Allergy Immunol, 150, pp.210~20 (2009)
- Cundall M, "Neutrophil-derived matrix metalloproteinase-9 is increased in severe asthma and poorly inhibited by glucocorticoids", J Allergy Clin Immunol, 112, pp.1064~71 (2003)
- Zhang GS, "New insights into the effects of Mycobacterium bovis Bacillus Calmette-Gue´rin on asthma", Chin Med J, 122, pp.577~83 (2009)
- 유진호, "BCG 백신 접종과 알레르기질환과의 관계", 대한소아알레르기및호흡기학회지, 18(2), pp.58~64 (2008), 대한 소아알레르기 호흡기학회