혈청 선별 검사에서 개별 혈청과 혼합 혈청의 면역 반응성 비교 분석
Comparative Analysis of Immunoreactivity between Individual Serum and Pooled Serum in Serum Screening
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
목적:개별 혈청과 혼합 혈청을 이용한 immunoblotting을 비교함으로써 유전자 재조합 식품에 새로이 발현된 단백질에 대한 혈청 선별 검사에서 개별 혈청 대신 혼합 혈청을 사용할 수 있을지를 알아보고자 하였다. 방법:ImmunoCAP (Pharmacia, Uppsala, Sweden) 검사상 난백 특이 immunoglobulin E (IgE)에 양성을 보인 환아 15명과 땅콩 특이 IgE에 양성을 보인 환아 12명의 혈청을 확보하였다. 특이 IgE 수준에 따른 반응의 차이를 비교하기 위해 환자의 땅콩 특이 IgE 수준이 10 kU/L이하와 10 kU/L 이상, 계란은 5 kU/L 미만, 5–10 kU/L, 11kU/L 이상으로 나누어 땅콩 및 난백 단백질 추출물과 immunoblotting을 실시하였다. 결과:땅콩 특이 IgE 수준이 10 kU/L 이상이나 이하에 상관 없이 개별 혈청에서 반응하였던 단백질이 혼합 혈청과의 반응에서도 모두 선명하게 감지되었다. 난백 특이 IgE 양성인 혈청과 난백과의 반응에서도 특이 IgE 수준에 관계없이 모든 군에서 개별 혈청에 나타났던 양성 반응을 혼합 혈청에서도 확인할 수 있었다. 결론:혈청 내 특이 IgE와 특정 단백질 간의 반응성을 관찰하는 데에는 특이 IgE 값에 관계없이 개별 혈청 대신 혼합 혈청을 사용해도 될 것으로 판단된다.
Abstract
Purpose:Serum screening test to detect specific immunoglobulin E (IgE) is an important step for the assessment of potential allergenicity of genetically modified (GM) food. The purpose of this study was to evaluate the usefulness of pooled serum for serum screening instead of individual serum. Methods:Children with allergic disease were recruited and those who were sensitized to peanut or egg white were selected to obtain their sera. Sensitization to these foods was determined when the level of specific IgE was over 0.35 kU/L by ImmunoCAP. The patients were divided into subgroups according to their level of specific IgE. Raw proteins were extracted and immunoblot analysis was performed to compare the immunoreactivity between individual serum and pooled serum. Results:Pooled serum from peanut-sensitized allergic children showed all the bands which were shown in immunoblot analysis by using individual serum and peanut protein extract. These findings were demonstrated both in pooled serum with low level of peanut-specific IgE and in those with high level of peanut-specific IgE. Likewise, there was no difference in the immunoreactivity between individual serum and pooled serum from egg white-sensitized allergic children. Conclusion:Pooled serum can be used as an alternative to individual serum for the serum screening in the allergenicity assessment of GM food.
참고문헌 (21)
- Metcalfe DD, "Genetically modified crops and allergenicity", Nat Immunol, 6, pp.857~860 (2005)
- Taylor SL, "Review of the development of methodology for evaluating the human allergenic potential of novel proteins", Mol Nutr Food Res, 50, pp.604~609 (2006)
- Goodman RE, "Allergenicity assessment of genetically modified crops: what makes sense?", Nat Biotechnol, 26, pp.73~81 (2008)
- Ladics GS, "Current codex guidelines for assessment of potential protein allergenicity", Food Chem Toxicol, 46(Suppl 10), pp.20~23 (2008)
- Ladics GS, "Identifying food proteins with allergenic potential: evolution of approaches to safety assessment and research to provide additional tools", Regul Toxicol Pharmacol, 54(3 Suppl), pp.2~6 (2009)
- Selgrade MK, "Safety assessment of biotechnology products for potential risk of food allergy: implications of new research", Toxicol Sci, 110, pp.31~39 (2009)
- Thomas K, "Scientific advancement of novel protein allergenicity evaluation: an overview of work from the HESI Protein Allergenicity Technical Committee (2000-2008)", Food Chem Toxicol, 47, pp.1041~1050 (2009)
- Liu Y, "Study on detecting antibodies to Toxoplasma gondii in pooled serum of blood donors by Dot- IGSS", Southeast Asian J Trop Med Public Health, 32, pp.558~561 (2001)
- Sadiq ST, "Pooling serum samples may lead to loss of potential biomarkers in SELDI-ToF MS proteomic profiling", Proteome Sci, 6, pp.16~ (2008)
- Howanitz PJ, "Prevalence of antibodies to HTLV-III in quality-assurance sera", Clin Chem, 32, pp.773~777 (1986)
- Cahoon-Young B, "Sensitivity and specificity of pooled versus individual sera in a human immunodeficiency virus antibody prevalence study", J Clin Microbiol, 27, pp.1893~1895 (1989)
- Liu P, "A prospective study of a serum-pooling strategy in screening blood donors for antibody to hepatitis C virus", Transfusion, 37, pp.732~736 (1997)
- Gizzarelli F, "Evaluation of allergenicity of genetically modified soybean protein extract in a murine model of oral allergen- specific sensitization", Clin Exp Allergy, 36, pp.238~248 (2006)
- Taylor SL, "Genetically engineered foods: implications for food allergy", Curr Opin Allergy Clin Immunol, 2, pp.249~252 (2002)
- Prescott VE, "Genetically modified plants and food hypersensitivity diseases: usage and implications of experimental models for risk assessment", Pharmacol Ther, 111, pp.374~383 (2006)
- Batista R, "Lack of detectable allergenicity of transgenic maize and soya samples", J Allergy Clin Immunol, 116, pp.403~410 (2005)
- Hoff M, "Serum testing of genetically modified soybeans with special emphasis on potential allergenicity of the heterologous protein CP4 EPSPS", Mol Nutr Food Res, 51, pp.946~955 (2007)
- Yum HY, "Genetically modified and wild soybeans: An immunologic comparison", ALLERGY AND ASTHMA PROCEEDINGS, 26(3), pp.210~216 (2005)
- 김상하, "Evaluating the Allergic Risk of Genetically Modified Soybean", Yonsei Medical Journal, 47(4), pp.505~512 (2006), 연세대학교의과대학
- 김현희, "소아 천식에서 호기 일산화질소 농도의 임상적 의의", Allergy Asthma & Respiratory Diseases, 19(3), pp.205~208 (2009), 대한 소아알레르기 호흡기학회
- Jae-Hwan Kim, "Allergenicity Assessment of Cry Proteins in Insect-resistant Genetically Modified Maize Bt11, MON810, and MON863", Food Science and Biotechnology, 18(5), pp.1273~1278 (2009), 한국식품과학회