혈청을 이용한 제초제내성 유전자재조합식품의 알레르기성 평가
Assessment of Allergenicity in Genetically Modified Herbicide-Resistant Foods Using the Serum Screening Test
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
목 적 : 유전자재조합 식품 보급이 확대됨에 따라 국내 알레르기 소아 환자의 혈청을 이용하여 유전자재조합 식품에 대한 알레르기 안전성을 평가하고자 하였다. 방 법 : 우리나라에서 승인된 제초제내성 유전자재조합식품(콩, 옥수수, 캐놀라)에 삽입되어 있는 신규단백질(CP4 EPSPS, PAT, BAR)를 분리 정제한 후 콩, 옥수수, 땅콩에 감작되어 있는 소아 알레르기 환자의 혈청과 immunoblot analysis를 시행함으로써 신규단백질에 대한 IgE 반응성을 조사하였다. 결 과 : 콩에 감작되어 있는 50명의 혈청에서는 CP4 EPSPS와 아무런 반응을 보이지 않았다. 마찬가지로 옥수수(50명) 및 땅콩(20명)에 감작되어 있는 환자 혈청에서도 PAT 및 BAR에 대한 특이 IgE를 발견할 수 없었다. 결 론 : 알레르기 질환을 앓고 있는 소아를 대상으로 하여 제초제내성 유전자재조합식품의 삽입단백질(CP4 EPSPS, PAT, BAR)에 대한 혈청 선별검사를 시행한 결과 특이 IgE를 발견할 수 없었으며, 이는 이들 유전자재조합식품이 알레르기 질환을 유발할 위험성이 적다는 것을 보여준다. 그러나 유전자재조합식품의 삽입단백질이 알레르기 질환을 발생하지 않는다는 결론을 얻기 위해서는 더 많은 환자를 대상으로 하는 지속적인 평가가 요구되며, 향후 이러한 혈청 선별검사를 지속적이고 효율적으로 하기 위해서는 체계적인 혈청은행의 구축 및 운영이 필요하겠다.
Abstract
Purpose : This study aimed to evaluate the potential allergenicity of genetically modified (GM) herbicide-resistant food by using the serum screenning test. Methods : Children with allergic disease were recruited, and those who were sensitized to soybean, corn or peanut were selected to obtain their sera. Sensitization to these food allergens was determined when the level of specific IgE was over 0.35 kU/L using ImmunoCAP (Pharmacia, Uppsala, Sweden). Immunoblot analyses were performed for soybean (n=50), corn (n=50) and peanut (n=20). Newly inserted gene was sequenced and cloned from GM soy (Roundup Ready Soybean, Monsanto), GM corn (Bt 11, Syngenta) and GM canola (MS8/RF3 canola, Bayer CropScience). These proteins, such as CP4 EPSPS, PAT, and BAR, were expressed and purified for the serum screening test. Results : Immunoblot analysis using CP4 EPSPS and sera from soybean-sensitized children showed no bands. Likewise, sera from corn-sensitized children and PAT did not demonstrate IgE binding in immunoblot analysis. In addition, there were no reactions between BAR and sera from peanut-sensitized patients. Conclusion : The serum screening test using sera from allergic children and newly inserted protein (CP4 EPSPS, PAT and BAR) in GM soybean, GM corn and GM canola failed to show IgE binding in immunoblot analysis. The results of this study suggest that these newly inserted proteins may not cause allergic disease. Further studies using more sera from allergic children are needed to conclude the safety of herbicide-resistant GM food.
참고문헌 (17)
- Metcalfe DD, "Assessment of the allergenic potential of foods derived from genetically engineered crop plants", Crit Rev Food Sci Nutr, 36, pp.s165~s186 (1996)
- WHO Expert Consultation on Foods Derived from Biotechnology, "Joint FAO", pp.2001
- Codex Alimentarius Commission, "Alinorm 03", 34:Joint FAO, 47~60
- Metcalfe DD, "Genetically modified crops and allergenicity", Nat Immunol, 6, pp.857~860 (2005)
- Goodman RE, "Assessing genetically modified crops to minimize the risk of increased food allergy: a review", Int Arch Allergy Immunol, 137, pp.153~166 (2005)
- Spok A, "Suggestions for the assessment of the allergenic potential of genetically modified organisms", Int Arch Allergy Immunol, 137, pp.167~180 (2005)
- Goodman RE, "Allergenicity assessment of genetically modified crops-what makes sense?", Nat Biotechnol, 26, pp.73~81 (2008)
- "2007 ISAAA report on global status of biotech/GM crops"[URL]
- Okunuki H, "Increased digestibility of two products in genetically modified food (CP4 EPSPS and Cry1Ab) after preheating", Shokuhin Eiseigaku Zasshi, 43, pp.68~73 (2002)
- Harrison LA, "The expressed protein in glyphosate-tolerant soybean, 5-enolpyrubylshikimate-3-phosphate synthase from Agrobacterium sp. strain CP4, is rapidly digested in vitro and is not toxic to acutely gavaged mice", J Nutr, 126, pp.728~740 (1996)
- Herouet C, "Safety evaluation of the phosphinothricin acetyltransferase proteins encoded by the pat and bar sequences that confer tolerance to glufosinate-ammonium herbicide in transgenic plants", Regul Toxicol Pharmacol, 41, pp.134~149 (2005)
- Batista R, "Lack of detectable allergenicity of transgenic maize and soya samples", J Allergy Clin Immunol, 116, pp.403~410 (2005)
- 김상하, "Evaluating the Allergic Risk of Genetically Modified Soybean", Yonsei Medical Journal, 47(4), pp.505~512 (2006), 연세대학교의과대학
- Hoff M, "Serum testing of genetically modified soybeans with special emphasis on potential allergenicity of the heterologous protein CP4EPSPS", Mol Nutr Food Res, 51, pp.946~955 (2007)
- Takagi K, "Improved ELISA method for screening human antigen-specific IgE and its application for monitoring specific IgE for novel proteins in genetically modified foods", Regul Toxicol Pharmacol, 44, pp.182~188 (2006)
- Yum HY, "Genetically modified and wild soybeans: an immunologic comparison", Allergy Asthma Proc, 통권 : 26, pp.210~216 (2005)
- Thomas K, "The utility of an international sera bank for use in evaluating the potential human allergenicity of novel prote", Toxicol Sci, 97, pp.27~31 (2007)