성인 집먼지진드기 알레르기 환자에서 특이 IgE 결합 성분항원 비교
Comparison of IgE-binding components between 2 house dust mites in adult allergic patients
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Purpose: This study investigated the diff erences in the profi le of IgE-binding components between Dermatophagoides pteronyssi- nus (Dp) and Dermatophagoides farina (Df) in respiratory allergic patients sensitized to Dp/Df. Methods: Eighteen patients with respiratory allergic diseases having higher levels of serum specifi c IgE to Df compared to those to Dp (>twice) were enrolled. IgE-immunoblot analysis using Dp and Df extracts were used to compare the IgE binding components. Study subjects were classifi ed into 2 groups according to the results of IgE-immunoblot analysis: 6 subjects having IgE-binding com- ponents to group 1 and 2 allergens (group B) and 12 subjects not having them (group A). Results: Group A subjects were older (47.92±8.51 vs. 35.50±11.10, P=0.039) and males were dominant (75% vs. 0% P=0.009). IgE- immunoblot analysis demonstrated that all the group B subjects had IgE bindings to 2 major components, 14 and 25 kDa, while group A subjects had IgE bindings to high-molecular weight components ranging from 60–98 kDa. The enzyme-linked immuno- sorbent assay inhibition test showed a signifi cant inhibition with additions of Df, not with Dp in group B subjects. Serum specifi c IgE levels to Dp and Df were signifi cantly higher in group B than in group A, while its ratio (Df to Dp) was signifi cantly higher in group A. No diff erences were noted in clinical parameters, total IgE, or eosinophil cationic protein levels. Conclusion: Heterogeneity of IgE binding patterns to Dp and Df extracts was noted according to the ratio of serum specifi c IgE (Df/ Dp).
Abstract
Purpose: This study investigated the diff erences in the profi le of IgE-binding components between Dermatophagoides pteronyssi- nus (Dp) and Dermatophagoides farina (Df) in respiratory allergic patients sensitized to Dp/Df. Methods: Eighteen patients with respiratory allergic diseases having higher levels of serum specifi c IgE to Df compared to those to Dp (>twice) were enrolled. IgE-immunoblot analysis using Dp and Df extracts were used to compare the IgE binding components. Study subjects were classifi ed into 2 groups according to the results of IgE-immunoblot analysis: 6 subjects having IgE-binding com- ponents to group 1 and 2 allergens (group B) and 12 subjects not having them (group A). Results: Group A subjects were older (47.92±8.51 vs. 35.50±11.10, P=0.039) and males were dominant (75% vs. 0% P=0.009). IgE- immunoblot analysis demonstrated that all the group B subjects had IgE bindings to 2 major components, 14 and 25 kDa, while group A subjects had IgE bindings to high-molecular weight components ranging from 60–98 kDa. The enzyme-linked immuno- sorbent assay inhibition test showed a signifi cant inhibition with additions of Df, not with Dp in group B subjects. Serum specifi c IgE levels to Dp and Df were signifi cantly higher in group B than in group A, while its ratio (Df to Dp) was signifi cantly higher in group A. No diff erences were noted in clinical parameters, total IgE, or eosinophil cationic protein levels. Conclusion: Heterogeneity of IgE binding patterns to Dp and Df extracts was noted according to the ratio of serum specifi c IgE (Df/ Dp).
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