Comparison of Allergenic Properties among Commercially Available House Dust Mite Allergen Extracts in Korea
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초록
Precise allergy diagnosis and effective allergen specific immunotherapy are largely dependent on the quality of allergen extract. Anew extract of Dermatophagoides farinae was commercially developed by Prolagen. The allergenic properties of the new extractwere compared with those of other commercial products. The allergenic properties of the new extract were compared accordingto protein concentration, protein profiles, major allergen (Der f 1) contents, and allergenic potency to those for three commerciallyavailable extracts imported in Korea (Jubilant HollisterStier Allergy, Lofarma S.p.A., and Stallergenes Greer). Protein concentrationsvaried up to 2.62-fold (0.404 to 1.057 mg/mL), and Der f 1 contents varied up to 11.3-fold (3.597 to 40.688 μg/mL). Protein profiles of the extracts showed no major discrepancies, although there were some differences in SDS-PAGE band intensities,reflecting protein concentrations. Allergen potency ranged from 37038 to 60491 PAU/mL. The Prolagen product was highestin terms of protein concentration and allergen potency. The Lofarma product displayed Der f 1 content similar to that in Prolagen(19.4 μg/mg vs. 19.3 μg/mg). Endotoxin levels varied 8.9-fold (1020 to 8985 EU/mL). The newly developed house dust mite extractshowed equal or better allergenic properties than available commercial extracts. This new product may be useful for better diagnosticsand allergen-specific immunotherapeutics.
Abstract
Precise allergy diagnosis and effective allergen specific immunotherapy are largely dependent on the quality of allergen extract. Anew extract of Dermatophagoides farinae was commercially developed by Prolagen. The allergenic properties of the new extractwere compared with those of other commercial products. The allergenic properties of the new extract were compared accordingto protein concentration, protein profiles, major allergen (Der f 1) contents, and allergenic potency to those for three commerciallyavailable extracts imported in Korea (Jubilant HollisterStier Allergy, Lofarma S.p.A., and Stallergenes Greer). Protein concentrationsvaried up to 2.62-fold (0.404 to 1.057 mg/mL), and Der f 1 contents varied up to 11.3-fold (3.597 to 40.688 μg/mL). Protein profiles of the extracts showed no major discrepancies, although there were some differences in SDS-PAGE band intensities,reflecting protein concentrations. Allergen potency ranged from 37038 to 60491 PAU/mL. The Prolagen product was highestin terms of protein concentration and allergen potency. The Lofarma product displayed Der f 1 content similar to that in Prolagen(19.4 μg/mg vs. 19.3 μg/mg). Endotoxin levels varied 8.9-fold (1020 to 8985 EU/mL). The newly developed house dust mite extractshowed equal or better allergenic properties than available commercial extracts. This new product may be useful for better diagnosticsand allergen-specific immunotherapeutics.
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