The difference of metabolic profile between male and female zebrafish
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Various experiments using zebrafish have been highlighted recently in the scientific community. Because it is possible to conduct practical experiment from various neurological research to area of genetic study or toxicity experiment. However, gender difference effects are nearly not considered. If the gender differences of zebrafish are considered it is possible to obtain more accurate data. In this study, zebrafish which have different genders were compared each other with NMR-based metabolomics. The extracts of male and female zebrafish were measured by 600 MHz NMR spectrometer. Statistical analysis and target profiling were conducted. As a result, muscle related metabolites were observed in male zebrafish and nerve related metabolites were observed in female zebrafish.
Abstract
Various experiments using zebrafish have been highlighted recently in the scientific community. Because it is possible to conduct practical experiment from various neurological research to area of genetic study or toxicity experiment. However, gender difference effects are nearly not considered. If the gender differences of zebrafish are considered it is possible to obtain more accurate data. In this study, zebrafish which have different genders were compared each other with NMR-based metabolomics. The extracts of male and female zebrafish were measured by 600 MHz NMR spectrometer. Statistical analysis and target profiling were conducted. As a result, muscle related metabolites were observed in male zebrafish and nerve related metabolites were observed in female zebrafish.
참고문헌 (12)
- P. McGrath, , Drug Discovery Today, 13, pp.394~ (2008)
- H. Kawai, , Glia, 36, pp.406~ (2001)
- E. R. Lusczek, , Journal of the Pancreas, 14, pp.161~ (2013)
- D. Jacob, , Analytical and Bioanalytical Chemistry, 405, pp.5049~ (2013)
- 윤다혜, "Applications of NMR spectroscopy based metabolomics: a review", Journal of the Korean Magnetic Resonance Society, 17(1), pp.1~10 (2013), 한국자기공명학회[DOI]
- 박성진, "NMR Study of larger proteins using isotope labeling", Journal of the Korean Magnetic Resonance Society, 18(2), pp.47~51 (2014), 한국자기공명학회[DOI]
- E. G. Bligh, , Canadian Journal of Biochemistry and Physiology, 37, pp.911~ (1959)
- D. Pharmazie, , An International Journal of Pharmaceutical Sciences, 61, pp.218~ (2006)
- M. F. M. van Stijna, , Metabolism, 61, pp.1036~ (2012)
- J. Ghosn, , Antiviral therapy, 10, pp.543~ (2005)
- S. Carmans, , Journal of Neuroscience Research, 88, pp.2420~ (2010)
- R. M. Abra, , Lipids and lipid metabolism, 431, pp.631~ (1976)