본문 바로가기
KERD
논문KCI 등재

H2O2와 Aβ로 유도된 pc12 cell에서 生酸棗仁 수추출물의 항산화 및 항치매 효과

The Effects of Antioxidant and Anti-Alzheimer on Hydrogen peroxide and β-amyloid peptid-induced PC 12 cells by Semen Ziziphi Spinosae water extract

김진우|정은영|김대현|이성근|김태헌|이기상|윤종현|강형원|류영수|이상원
대한한방신경정신과학회 (The Korean Society Of Oriental Neuropsychiatry)|2008.11|동의신경정신과학회지 (The Korean Society of Oriental Neuropsychiatry)|제19권 제3호|pp. 179-194

2

조회

0

다운로드

2

인용

0

참고문헌

키워드

MTT assayAntioxidantAnti-AlzheimerSemen Ziziphi Spinosae(SZS)pc 12LDH assay
주제분류한의학

초록

Objective : The antioxidant and anti-Alzheimer effects of Semen Ziziphi Spinosae (SZS) water extract against the amyloid beta peptide (1-42) or H2O2-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods : The cells were incubated with SZS water extract and oxidative damage-inducing materials, amyloid beta peptide (1-42) or H2O2 for 24 h. The cellular viability was assessed by WST-1 assay, cytotoxic damage by LDH activity assay, oxidative damages of cells by fluorescence spectrophotometric method, and apoptosis by TUNEL staining assay. Results and Conclusions : 1. Preincubation of the cells with SZS water extract prior to amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) exposure elevated the cell survival close to the control and decreased the level of LDH activity and the fluorescence from the cell homogenates and TUNEL staining of the cells, compared to only amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) treated conditions . 2. Our study suggests that Semen Ziziphi Spinosae (SZS) water extract has protective effects against amyloid beta peptide (1-42) or H2O2-induced cell toxicity through the antioxidation mechanism, which might be beneficial for the treatment of Alzheimer's disease.

Abstract

Objective : The antioxidant and anti-Alzheimer effects of Semen Ziziphi Spinosae (SZS) water extract against the amyloid beta peptide (1-42) or H2O2-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods : The cells were incubated with SZS water extract and oxidative damage-inducing materials, amyloid beta peptide (1-42) or H2O2 for 24 h. The cellular viability was assessed by WST-1 assay, cytotoxic damage by LDH activity assay, oxidative damages of cells by fluorescence spectrophotometric method, and apoptosis by TUNEL staining assay. Results and Conclusions : 1. Preincubation of the cells with SZS water extract prior to amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) exposure elevated the cell survival close to the control and decreased the level of LDH activity and the fluorescence from the cell homogenates and TUNEL staining of the cells, compared to only amyloid beta peptide (1-42) (2 uM) or H2O2 (30 uM) treated conditions . 2. Our study suggests that Semen Ziziphi Spinosae (SZS) water extract has protective effects against amyloid beta peptide (1-42) or H2O2-induced cell toxicity through the antioxidation mechanism, which might be beneficial for the treatment of Alzheimer's disease.

참고문헌 (42)

  1. 대한신경학회, "신경학", pp.406~ (2007), 군자출판사
  2. 大韓韓方神經精神科學會, "韓方神經精神醫學", pp.311~320 (2005), 集文堂
  3. Cummings JL, "A Clinical Approach" (1992), Butterworth-Heinemann
  4. Coyle JT, "Oxidative stress. glutamete. and neurodegenerative disoders", Science, 262, pp.689~695 (1993)
  5. Markesbery WR, "Oxidative stress hypothesis in Alzheimer's disease", Free Radic Biol Med, 23, pp.134~147 (1997)
  6. Schippling S, "Increased lipoprotein oxidation in Alzheimer's disease", Free Radic Biol Med, 28, pp.351~360 (2000)
  7. Selcoe DJ, "Alzheimer's disease : a central role for amyloid", J Neuropathol Exp Neurol, 53(5), pp.438~447 (1994)
  8. Hardy J, "Genetic. dissection of Alzheimer's disease and related dementias:amyloid and its relationship to tau", Nat Neurosci, 1(5), pp.355~358 (1998)
  9. Seo J, "Effects of nicotine on APP secretion and A beta-or CT105-induced toxicity", Biol Psychiatry, 49(3), pp.240~247 (2001)
  10. Aust SD, "Free radicals in toxicology", Toxicology and Applied Pharmacology, 120, pp.168~178 (1993)
  11. Halliwell B, "Protection against oxidants in biological systems:the superoxide theory of oxygen toxicty. in free radicals in biology and medicine", pp.86~89 (1989), Clarendon Press
  12. Polidori MC, "Susceptibility to free radical-induced antioxidant consumption and lipid peroxidation is increased in very old subjects with Alzheimer disease", J Alzheimer Dis, 4(6), pp.517~522 (2002)
  13. 洪元植, "精校黃帝內經素問", pp.37~ (1985), 東洋醫學硏究院
  14. 張介賓, "景岳全書", pp.71~578 (1985), 上海科學技術出版社
  15. 陳士澤, "辨證錄", pp.241~246 (1989), 醫聖堂
  16. 최성열, 김태헌, 강형원, 류영수, 김대현, 김상태, "치매에 대한 최신 실험적 연구 동향 (2000년 이후 한의학 학술지를 중심으로)", 동의신경정신과학회지, 19(1), pp.125~146 (2008), 대한한방신경정신과학회
  17. 錢鏡湖, "辨證奇門全書", pp.222~225 (1990), 甘地出版社
  18. 辛民敎, "臨床本草學", pp.643~644 (2000), 영림사
  19. 강형원, "壯元丸加減方 水抽出物이 아밀로이드 전구단백질로 유도된 생쥐의 신경아세포주에서의 항치매 효과", 東醫神經精神科學會誌, 18(2), pp.13~24 (2007)
  20. 김상호, "天王補心丹과 單味들이 Hypoxia-Reoxgenation에 의해 손상받은 Mouse Neuroblastoma 2a Cells에 미치는 영향", 東醫神經精神科學會誌, 17(2), pp.15~36 (2006)
  21. 김건진, "壯元丹이 CT105와 Aβ로 誘導된 Alzheimer's Disease 病態 모델에 미치는 영향", 東醫神經精神科學會誌, 17(2), pp.91~122 (2006)
  22. 정정욱, "酸棗仁이 CT 105에 의한 신경세포상해 및 백석의 기억에 미치는 영향", 大韓本草學會誌, 20(2), pp.19~33 (2005)
  23. 이근후, "최신임상정신의학", pp.138~ (1988), 하나의학사
  24. 李東垣, "痴呆에 關한 東西醫學的 比較 考察", 大韓韓方內科學會誌, 16(1), pp.2~14 (1995)
  25. Downen M, "Neuronal death in cytokine-activated primary human brain cell culture ; role of tumor necrosis factor-alpha", Glia, 28(2), pp.114~127 (1999)
  26. Gomez-Isla T., "Profound loss of layer II entorhinal cortex neurons occurs in very mild Alzheimer`s disease", J Neurosci, 16(14), pp.4491~4500 (1996)
  27. Markesbery WR, "Brain trace elements in Alzheimer's disease. In Alzheimer's Disease", neurotoxicology, 7(1), pp.195~206 (1986)
  28. Pratico D, "Alzheimer's disease and oxygen radical. new insights", Biochem Pharmachol, 63, pp.563~567 (2002)
  29. 陳士澤, "增補百病辨証錄", pp.172~175 (1981), 書苑堂
  30. 嚴用和, "中國醫學大系11", pp.256~266 (1984), 여강출판사
  31. 全國韓醫科大學 本草學敎室, "本草學", pp.493~494 (1991), 永林社
  32. 이경순, "中藥大辭典", pp.2008~2092 (1997), 정담
  33. Ono K, "Anti-amyloidogenic effects of antioxidants: implications for the prevention and therapeutics of Alzheimer's disease", Biochim Biophys Acta, 1762(6), pp.575~586 (2006)
  34. Jang JH, "Beta-Amyloid induces oxidative DNA damage and cell death through activation of c-Jun N terminal kinase", Ann N Y Acad Sci, 973, pp.228~236
  35. Yu MH, "Physiological Activity", Nutrition : Components and Their Antioxidative Activities of Methanol Extracts from Sarcocarp and Seed of Zizyphus jujuba var, 2006, pp.38(1), 128~134
  36. 이원복, "아밀로이드 베타 단백질을 이용한 알츠하이머 모델에서 유도되는 신경세포 사멸에 대한 피브로인 BF-7의 뇌기능 보호 작용에 관한 연구", 대한해부학회지, 40(1), pp.57~67 (2007), 대한해부학회
  37. Emerit J, "Neurodegenerative disease and oxidative stress", Biomedicine and Pharmacotherapy, 58, pp.39~46 (2003)
  38. Chu CT, "Oxidative neuronal injury", Eur J Biochem, 271, pp.2060~2066 (2004)
  39. Mattson MP, "Neurodegenerative disorders and ischemic brain disease", Apoptosis, 6, pp.69~81 (2001)
  40. Andersen JK, "Oxidative stress in neurodegeneration: Cause or consequence?", Nature Neurosci, 1, pp.518~523 (2004)
  41. 洪元植, "精校黃帝內經靈樞", pp.79~ (1958), 東洋醫學硏究院
  42. 陳士澤, "國譯石室秘錄", pp.102~ (1984), 書苑堂

관련 논문AI추천

H2O2로 유도된 산화적 스트레스에 대한 壯元丸加減方의 PC 12 cell 에서의 항산화 효과강형원, 김태헌, 류영수, 박용훈, 손일홍, 이상원, 임정현대한한방신경정신과학회 · 동의신경정신과학회지2009 · 제20권 제2호아밀로이드베타 펩타이드 유도성 신경세포독성에 대한 녹차 추출물의 보호 효과김영인, 김재겸, 박정윤, 이승철, 정창호, 조성환, 최성길, 최수정, 허호진한국식품저장유통학회 · Food Science and Preservation2008 · 제15권 제5호六鬱湯의 산화적 스트레스에 대한 뇌세포 보호효과이경섭, 이진무, 이창훈, 장준복, 정선형, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제1호Inhibitory Effect of Chaenomeles sinensis Fruit on Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice성연희, 송경식, 정명환한국약용작물학회 · 한국약용작물학회지2012 · 제20권 제1호마가목 수피 추출물의 아밀로이드 베타 단백질로 유도된 신경독성에 대한 학습 및 기억력 개선 효과김종민, 김지혜, 배동혁, 허호진한국식품저장유통학회 · Food Science and Preservation2022 · 제29권 제2호人蔘養營湯의 산화적 stress에 대한 뇌세포 보호효과김승현, 이경섭, 이진무, 이창훈, 장준복, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제1호

이 간행물의 다른 논문 (23건)

긍정심리학과 한의학김정호pp. 1-22한의과대학생들의 자아방어기제 특성에 대한 예비적 연구조성훈, 조혜영, 황의완, 김윤용, 김지영pp. 23-34심박변이도 및 SCL-90-R 측정을 통한 자율훈련법과 과일 명상의 효과 비교 분석김종우, 김지영, 황은영, 정선용, 서진우pp. 35-44七情傷으로 인한 두통 환자의 심박변이도(Heart Rate Variabillity)에 관한 고찰고경모, 박선영, 정대규, 최철홍pp. 45-54五子地黃飮子 열수추출물과 초미세분말이 싸이토카인과 건망증 생쥐모델 기억력감퇴에 미치는 영향이상룡, 김석환pp. 55-68
원문 보기