In Vivo Micronucleus Test of Methylcyclopentane and 1,4-Dichlorobutane
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We investigated the genotoxicity of two chemicals, methylcyclopentane and 1,4-dichlorobutane with in vivo micronucleus test. Although these two chemicals have already been tested many times, a micronucleus test has not been conducted and the usage of these chemicals has been recently increased. 7 week male ICR mice were tested at dosages of 500, 1,000, and 2,000 mg/kg for methylcyclopentane and 500, 1,000, and 2,000 mg/kg for 1,4-dichlorobutane, respectively. After 24 hours of oral administration with the two chemicals, the mice were sacrificed and their bone marrow cells were prepared for smearing slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocytes (PCE), all treated groups expressed no statistically significant increase of MNPCE compared to the negative control group. There were no clinical signs related with the oral exposure of these two chemicals. It was concluded that the two chemicals did not induce micronucleus in the bone marrow cells of ICR mice, and there was no direct proportion with dosage. These results indicate that the two chemicals have no mutagenic potential under each study condition.
Abstract
We investigated the genotoxicity of two chemicals, methylcyclopentane and 1,4-dichlorobutane with in vivo micronucleus test. Although these two chemicals have already been tested many times, a micronucleus test has not been conducted and the usage of these chemicals has been recently increased. 7 week male ICR mice were tested at dosages of 500, 1,000, and 2,000 mg/kg for methylcyclopentane and 500, 1,000, and 2,000 mg/kg for 1,4-dichlorobutane, respectively. After 24 hours of oral administration with the two chemicals, the mice were sacrificed and their bone marrow cells were prepared for smearing slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocytes (PCE), all treated groups expressed no statistically significant increase of MNPCE compared to the negative control group. There were no clinical signs related with the oral exposure of these two chemicals. It was concluded that the two chemicals did not induce micronucleus in the bone marrow cells of ICR mice, and there was no direct proportion with dosage. These results indicate that the two chemicals have no mutagenic potential under each study condition.
참고문헌 (16)
- Sigma-Aldrich Co, "MSDS of Methylcyclopentane" (2010)
- Grandjean P, "Developmental neurotoxicity of industrial chemicals", Lancet, 16(368), pp.2167~2178 (2006)
- National Occupational Exposure Survey (NOES) con ducted from 1981-1983, "NIOSH", Estimated numbers of employees potentially exposed to specific agents by 2-digit standard industrial classification, ~ (1983)
- Rappaport SM, "Exposure hydrocarbon components of gasoline in the petroleum industry", Appl Ind Hyg, 2, pp.148~154 (1987)
- Coleman EC, "Isolation and identification of volatile compounds from baked potatoes", J Agric Food Chem, 29, pp.42~48 (1981)
- Sigma-Aldrich Co, "MSDS of 1,4-dichloropentane" (2010)
- GLP studies database with methylcyclopentane, "National Toxicology Program Database Search", Research Triangle Park, ~ (2010)
- GLP studies database with 1,4-dichlorobutane, "National Toxicology Program Database Search", Research Triangle Park, ~ (2010)
- Douglas GR, "How well do in vitro tests predict in vivo genotoxicity", Environ Mutagen, 7(3), pp.31~ (1985)
- Guidelines for Testing of Chemicals, Test Guideline 474: Mammalian Erythrocyte Micronucleus Test, "Organization for Economic Operation and Development", 1~10 (1997)
- Scott D, "X-ray-induced chromosomal aberrations in vicia faba: changes in response during the cell cycle", Mutat Res, 4, pp.579~599 (1967)
- Ono Y, "A comparative study on the toxicity of n-hexane and its isomers on the peripheral nerve", Int Arch Occup Environ Health, 48, pp.289~294 (1981)
- Conkle JP, "Trace composition of human respiratory gas", Arch Environ Health, 30, pp.290~295 (1975)
- Fenech M, "Sex is an important variable affecting spontaneous micronucleus frequency in cytokinesisblocked lymphocytes", Mutat Res, 313, pp.203~207 (1994)
- Vanparys P, "The micronucleus assay as a test for the detection of aneugenic activity", Mutat Res, 244, pp.95~103 (1990)
- Kim SJ, "A Study of Micronucleus Induction with Methyl Formate and 2-Methylbutane in Bone Marrow Cells of Male ICR Mice", Safety and Health at Work, 1(1), pp.80~86 (2010)