A Study of Micronucleus Induction with 3 Chemicals in Bone Marrow Cells of ICR Mice: Octadecanamide, 3-chloro-2-hyrdoxypropansulfonic acid sodium salt, 2,4,7,9-tetramethyl-5-decyne-4,7-diol

Abstract

We investigated the genotoxicity of three chemicals, octadecanamide, 3-chloro-2-hyrdoxypropansulfonic acid sodium salt and 2,4,7,9-tetramethyl-5-decyne-4,7-diol, using male ICR mice bone marrow cells for the screening of micronucleus induction. Although these three chemicals have already been tested numerous times,a micronucleus test has not been conducted. 7 week male ICR mice were tested at dosages of 500, 1,000, and 2,000 mg/kg for the 3 chemcals, respectively. After 24 hours of oral administration with the three chemicals,the mice were sacrificed and their bone marrow cells were prepared for smearing slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocytes (PCE), all treated groups expressed no statistically significant increase of MNPCE compared to the negative control group. There were no clinical signs related with the oral exposure of these three chemicals. It was concluded that these three chemicals did not induce micronucleus in the bone marrow cells of ICR mice, and there was no direct proportion with dosage. These results indicate that the three chemicals have no genotoxic potential under each study condition.