ICR 마우스에서 황칠 추출물의 유전독성 및 단회 투여 급성 독성
Genotoxicity and Single Oral Dose Toxicity of Dendropanax morbifera extract in ICR Mice
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The current study evaluated the safety of Dendropanax morbifera extract in the ICR mouse by testing both genotoxicity and single-dose toxicity of the compound. Compared to a negative control, the micronucleus test indicated no significant increase in the number of micronucleus polychromatic erythrocytes (MNPCE) in polychromatic erythrocytes (PCE), detected in the Dendropanax morbifera extract at 250, 500, or 750 mg/kg. Similarly, there were no changes in the rate of PCE/(PCE+NCE) in Dendropanax morbifera extract compared to the negative control group, though there was a significant decrease in the positive control group (p<0.05). In the single oral dose toxicity test, there were no significant changes in clinical signs, body weight, organ weights or gross observation compared to the negative control group. The serum biochemical examination indicated that compared to the negative control group, the ALP level was significantly increased in female mice at doses of 500 and 750 mg/kg of Dendropanax morbifera extract (p<0.05), however there was no significant difference in the correlated liver index test items such as AST and ALP. Histopathological observations revealed no change between treatment and negative control groups in liver or kidney tissues. Taken together, these results found no observed adverse effect level (NOAEL) of the single oral application of Dendropanax morbifera extract up to 750 mg/kg in mice. Based on these findings, we suggest that Dendropanax morbifera could be used as a non-toxic natural material for functional cosmetics, food, and medicine development.
Abstract
The current study evaluated the safety of Dendropanax morbifera extract in the ICR mouse by testing both genotoxicity and single-dose toxicity of the compound. Compared to a negative control, the micronucleus test indicated no significant increase in the number of micronucleus polychromatic erythrocytes (MNPCE) in polychromatic erythrocytes (PCE), detected in the Dendropanax morbifera extract at 250, 500, or 750 mg/kg. Similarly, there were no changes in the rate of PCE/(PCE+NCE) in Dendropanax morbifera extract compared to the negative control group, though there was a significant decrease in the positive control group (p<0.05). In the single oral dose toxicity test, there were no significant changes in clinical signs, body weight, organ weights or gross observation compared to the negative control group. The serum biochemical examination indicated that compared to the negative control group, the ALP level was significantly increased in female mice at doses of 500 and 750 mg/kg of Dendropanax morbifera extract (p<0.05), however there was no significant difference in the correlated liver index test items such as AST and ALP. Histopathological observations revealed no change between treatment and negative control groups in liver or kidney tissues. Taken together, these results found no observed adverse effect level (NOAEL) of the single oral application of Dendropanax morbifera extract up to 750 mg/kg in mice. Based on these findings, we suggest that Dendropanax morbifera could be used as a non-toxic natural material for functional cosmetics, food, and medicine development.
참고문헌 (29)
- Beak WB, "Biological activity studies of Korea specialties Dendropanax morbifera", Doctor, Department of Hygienic Chemistry, pp.2003
- 정상원, "모시잎추출물의 단회 · 반복투여 독성 및 복귀돌연변이능 평가", KSBB Journal, 32(4), pp.256~264 (2017), 한국생물공학회[DOI]
- El-Bayoumy K, "The protective role of selenium on genetic damage and on cancer", Mutat Res, 475, pp.123~139 (2001)
- FDA Guidance for Industry, Estimating the maximum safe starting dose in initial clinical trials for therapeutics in adult healthy volunteers, "FDA", FDA, 1~27 (2005)
- 홍충의, "생강나무(Lindera obtusiloba Blume) 잎 70% 에탄올 추출물의 단회와 14일 반복투여 독성시험의 안전성 평가", 한국식품영양과학회지, 38(10), pp.1324~1330 (2009), 한국식품영양과학회
- Hyun TK, "Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Léveille", Food Chem, 141, pp.1947~1955 (2013)
- 임규정, "황칠나무 줄기 추출물의 MCF-7과 MDA-MB-231 유방암 세포주에 대한 세포증식억제 효과", 대한한방부인과학회지, 28(2), pp.26~39 (2015), 대한한방부인과학회[DOI]
- 지정훈, "퍼머넌트 웨이브 시술시 황칠나무 잎 트리트먼트가 모발에 미치는 효과", 대한미용학회지, 11(3), pp.207~213 (2015), 대한미용학회[DOI]
- Kim HR, "Chemical characteristics of the leaves and the seeds of Korean Dendropanax (Dendropanax morbifera Lev.)", J Korean Soc Appl Bl, 43, pp.63~66 (2000)
- 김진솔, "황칠 추출물을 첨가한 청국장이 마우스의 면역기능에 미치는 효과", 한국산학기술학회논문지, 20(6), pp.430~438 (2019), 한국산학기술학회[DOI]
- Kim WS, "Dendropanax morbifera Léveille extract facilitates cadmium excretion and prevents oxidative damage in the hippocampus by increasing antioxidant levels in cadmium-exposed rats", BMC Complement Altern Med, 14, pp.428~ (2014)
- Kwan YP, "Acute and subchronic toxicity study of Euphorbia hirta L. methanol extract in rats", Biomed Res Int, 182064, pp.1~14 (2013)
- Kwon CY, "A study on awareness of organic cosmetics and preference", Master, Department of Cosmetic and Beauty, pp.2010
- 이창용, "황칠나무 추출물의 항산화 및 간세포보호효과", 생약학회지, 50(1), pp.32~36 (2019), 한국생약학회
- Lewis RW, "Recognition of adverse and nonadverse effects in toxicity studies", Toxicol Pathol, 30, pp.66~74 (2002)
- 임정묵, "화장품 소재 개발을 위한 대두 추출물의 생리활성 평가", 대한미용학회지, 16(1), pp.11~22 (2020), 대한미용학회[DOI]
- Mei N, "DNA adduct formation and mutation induction by aristolochic acid in rat kidney and liver", Mutat Res, 602, pp.83~91 (2006)
- Moon HI, "Antidiabetic effects of dendropanoxide from leaves of Dendropanax morbifera Leveille in normal and streptozotocin-induced diabetic rats", Hum Exp Toxicol, 30, pp.870~875 (2010)
- 김선오, "흰쥐에서 황칠나무 열수 추출물을 포함한 혼합물의 혈중 알코올 농도와 숙취 해소 효과", Journal of Chitin and Chitosan, 23(4), pp.267~276 (2018), 한국키틴키토산학회[DOI]
- OECD Guidelines for the testing of chemicals (Guideline 474): Mammalian erythrocyte micronucleus test, "OECD", OECD, 1~21 (2014)
- 박수아, "황칠나무 잎 추출물의 세포 항산화 활성과 미백활성 측정", 한국미생물·생명공학회지, 41(4), pp.407~415 (2013), 한국미생물·생명공학회[DOI]
- 박세호, "사염화탄소로 유발된 산화적 손상에 대한 황칠나무 잎 추출물의 간세포 보호 효과", 생명과학회지, 30(4), pp.370~378 (2020), 한국생명과학회[DOI]
- Park YM, "A study on the utilization of Dendropanax morbifera Lev. leaf extract for material of functional cosmetics and hair growth products", Doctor, Department of Cosmetology Science, pp.2017
- Renner HW, "In vivo effects of single or combined dietary antimutagens on mutagen-induced chromosomal aberrations", Mutat Res, 244, pp.185~188 (1990)
- Shin JE, "A study of consumers’ perception and behaviors on cosmetic safety", Master, Department of Cosmetic and Beauty, pp.2012
- 성익재, "麻黃附子細辛湯의 마우스 단회 경구투여 독성 및 골수세포를 이용한 유전독성 평가", 동의생리병리학회지, 24(1), pp.124~133 (2010), 한의병리학회
- Surveswaran S, "Systematic evaluation of natural phenolic antioxidants from 133 indian medicinal plants", Food Chem, 102, pp.938~953 (2007)
- Yang JW, "Protective effect of hot water extract of Dendropanax morbifera Lev. on ethanol-induced liver damage", Doctor, Department of Medicine, pp.2010
- Yu, Hai Yang, "Anti-cancer and anti-inflammatory activities of natural compounds isolated from Hyul-Tong-Ryung and Dendropanax morbifera", 박사, 의생명과학과, pp.2012