본문 바로가기
KERD
논문KCI 등재

트레드밀 운동과 밝은 빛이 흰쥐 해마의 GABAA 수용체와 Cdk5 발현에 미치는 영향

Effects of Treadmill Exercise and Bright Light on Expression of GABAA receptor and Cdk5 in Rat Hippocampus

0

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류체육

초록

지속적인 운동과 햇빛 치료는 스트레스에 대한 저항력을 높여주고, 우울과 불안을 감소시키며, 이러한 과정은 신경전달물질 GABA의 분비에 따라 뇌의 신경세포의 성장 및 분화 그리고 발달과정에 관여한다. 본 연구는 트레드밀 운동과 밝은 빛이 흰쥐의 해마에서 GABAA 수용체의 활성에 의해 Ca2+이 세포내 유입이 되면 calcineurin의 칼슘 의존성 경로의 활성을 통해 NFATc4 단백질 및 뇌신경세포 발달과정에 관여하는 Cdk5 단백질과 대부분의 Cdk와는 달리 신경세포 특이적 조절인자인 p35의 변화가 신경세포 생성에 미치는 영향을 알아보고자 면역얼룩법을 수행하였다. 그 결과 GABAA 수용체, Cdk5 그리고 p35는 통제집단과 빛집단에 비해, KCC2와 NFATc4는 다른 모든 집단에 비해, calcineurin은 통제집단에 비해 운동+빛집단에서 유의미하게 증가함을 알 수 있었다. 결과적으로 운동과 빛의 복합적 치료는 뇌의 해마에서 뇌신경세포 발달 과정에 관여함으로써 뇌기능을 증진시키는데 긍정적인 효과가 있음을 확인하였다.

Abstract

It has been reported that continuous exercise and bright light exposure improved resistance to stress and reduced depression and anxiety, which were attributed to enhancing neurotransmitter GABA exocytosis and stimulating neurogenesis. In this study, the effects of treadmill exercise and bright light exposure on the expression of GABAA receptor activity, calcineurin and calcineurin calcium signaling pathway-depended NFATc4, neurogenesis-related protein Cdk5 and specific regulator factor of Cdk in neurogenesis p35 in the hippocampus of rats were investigated by western blot assay. The expression of GABAA receptor, Cdk5 and p35 significantly increased in the exercise+light group compared to the control group and the light group. The expression of KCC2 and NFATc4 significantly increased in the exercise+light group compared to all the other groups. And the expression of calcineurin significantly increased in the exercise+light group compared to the control group. The overall results showed that exercise and bright light stimulated neurogenesis of the hippocampus in rats and had positive effects on improving the brain neuronal function.

참고문헌 (47)

  1. Akimoto, T., "Exercise stimulates Pgc-1alpha transcription in skeletal muscle through activation of the p38 MAPK pathway", Journal of Biological Chemistry, 280(20), pp.19587~19593 (2005)
  2. Baksh, S., "The role of calcineurin in lymphocyte activation", Seminars in Immunology, 12(4), pp.405~415 (2000)
  3. Brambilla, P., "GABAergic dysfunction in mood disorders", Molecular Psychiatry, 8(8), pp.721~737 (2003)
  4. Crabtree, G. R., "NFAT signaling:choreographing the social lives of cells", Cell, 109, pp.67~79 (2002)
  5. Crowley, K., "The effects of normal aging on sleep spindle and K-complex production", Clinical Neurophysiology, 113(10), pp.1615~1622 (2002)
  6. Dean, E., "Time to see the light", Nursing Standard, 26(31), pp.20~21 (2012)
  7. Eriksson, P. S., "Neurogenesis in the adult human hippocampus", Nature Medicine, 4(11), pp.1313~1317 (1998)
  8. Felice, D., "Blockade of the GABA(B)receptor increases neurogenesis in the ventral but not dorsal adult hippocampus : relevance to antidepressant action", Neuropharmacology, 63(8), pp.1380~1388 (2012)
  9. Gagné, A. M., "Impact of light therapy on rod and cone functions in healthy subjects", Psychiatry Research, 151(3), pp.259~263 (2007)
  10. Goto, S., "The distribution of calcineurin in rat brain by light and electronmicroscopic immunohistochemistry and enzyme-immunoassay", Brain Research, 397(1), pp.161~172 (1986)
  11. Gould, E., "Hippocampal neurogenesis in adult Old World primates", Proceedings of the National Academy of Sciences of the United States of America, 96(9), pp.5263~5267 (1999)
  12. Gould. E., "Neurogenesis in the neocortex of adult primates", Science, 286(5439), pp.548~552 (1999)
  13. Graef, I. A., "Neurotrophins and netrins require calcineurin", NFAT signaling to stimulate outgrowth of embryonic axons, 2003, pp.113(5), 657~670
  14. Harvey, B. H., "NMDA receptor involvement in imipramine withdrawal-associated effects on swim stress, GABA levels and NMDA receptor binding in rat hippocampus", Life Sciences, 71(1), pp.43~54 (2002)
  15. Hellmich, M. R., "Neuronal cdc2-like kinase : a cdc2-related protein kinase with predominantly neuronal expression", Proceedings of the National Academy of Sciences of the United States of America, 89(22), pp.10867~10871 (1992)
  16. Ishida, A., "Light activates the adrenal gland : timing of gene expression and glucocorticoid release", Cell Metabolism, 2(5), pp.297~307 (2005)
  17. Kaplan, M. S., "Neurogenesis in the 3-month-old rat visualcortex", Journal of Comparative Neurology, 195(2), pp.323~338 (1981)
  18. Kaplan, M. S., "Neurogenesis in the adult rat : electron microscopic analysis of lightradioautographs", Science, 197(4308), pp.1092~1094 (1977)
  19. Kim, Y. P., "Magnitude and time dependence of the effect of treadmill exercise on cell proliferation in the dentate gyrus of rats", International Journal of Sports Medicine, 24(2), pp.114~117 (2003)
  20. Kin, H., "Embryonic development of GABAergic signaling in the mouse spinal trigeminal nucleus interpolaris", Neuroscience Letters, 566, pp.221~225 (2014)
  21. Kwon, S. J., "Low-intensity treadmill exercise and", or bright light promote neurogenesis in adult rat brain, 2013, pp.8(10), 922~929
  22. 권성진, "트레드밀 운동과 밝은 빛이 흰쥐 뇌의 세로토닌 발현에 미치는 영향", 체육과학연구, 25(2), pp.193~201 (2014), 국민체육진흥공단 부설 한국스포츠개발원
  23. 권성진, "복합운동처치가 흰쥐 해마의 PSD95-NMDA 수용체 변화와 신경영양인자에 미치는 영향", 한국체육학회지, 53(2), pp.509~522 (2014), 한국체육학회
  24. 권성진, "Low-intensity treadmill exercise and bright light upregulate brain-derived neurotrophic factor expression and intracellular signaling pathway in rat hippocampus and cerebral cortex", Animal Cells and Systems, 18(2), pp.135~142 (2014), 한국통합생물학회[DOI]
  25. Lawson. V. H., "The role of the septohippocampal pathway in the regulation of hippocampal field activity and behavior : analysis by the intraseptal microinfusion of carbachol, atropine, and procaine", Experimental Neurology, 120(1), pp.132~144 (1993)
  26. Lee, K. Y., "Interaction of cyclindependent kinase 5(Cdk5)and neuronal Cdk5 activator in bovinebrain", Journal of Biological Chemistry, 271(3), pp.1538~1543 (1996)
  27. Levine, E. S., "Brain-derived neurotrophic factor increases activity of NR2B-containing N-methyl-D-aspartate receptors in excised patches from hippocampal neurons", Journal of Neuroscience Research, 62(3), pp.357~362 (2000)
  28. Lew, J., "A brain-specific activator of cyclin-dependent kinase 5", Nature, 371(6496), pp.423~426 (1994)
  29. 서진희, "Treadmill exercise prevents GABAergic neuronal loss with suppression of neuronal activation in the pilocarpine-induced epileptic rats", Journal of exercise rehabilitation, 11(2), pp.80~86 (2015), 한국운동재활학회[DOI]
  30. Lips, P., "Vitamin D physiology", Progress in Biophysics and Molecular Biology, 92(1), pp.4~8 (2006)
  31. Matsui, H., "Immunohistochemical localization of calcineurin, calmodulinstimulated phosphatase, in the rat hippocampus using a monoclonal antibody", Brain Research, 402(1), pp.193~196 (1987)
  32. Molteni, R., "Differential effects of acute and chronic exercise on plasticity-related genes in the rat hippocampus revealed by microarray", European Journal of Neuroscience, 16(6), pp.1107~1116 (2002)
  33. Poon, R. Y., "Identication of functional domains in the neuronal Cdk5 activator protein", Journal of Biological Chemistry, 272(9), pp.5703~5708 (1997)
  34. Rojas, J. C., "Neuroprotective effects of near-infrared light in an in vivo model of mitochondrial optic neuropathy", Journal of Neuroscience, 28(50), pp.13511~13521 (2008)
  35. Schmidt-Hieber, C., "Enhanced synaptic plasticity in newly generated granule cells of the adult hippocampus", Nature, 429(6988), pp.184~187 (2004)
  36. Shaw, V. E., "Neuroprotection of midbrain dopaminergic cells in MPTP-treated mice after near-infrared light treatment", Journal of Comparative Neurology, 518(1), pp.25~40 (2010)
  37. Shiah, I. S., "GABA function in mood disorders : an update and critical review", Life Sciences, 63(15), pp.1289~1303 (1998)
  38. Stranahan, A. M., "Social isolation delays the positive effects of running on adult neurogenesis", Nature Neuroscience, 9(4), pp.526~533 (2006)
  39. Sved, A. F., "Endogenous GABA acts on GABAB receptors in nucleus tractus solitarius to increase blood pressure", Brain Research, 526(2), pp.235~240 (1990)
  40. Takatsu, S., "Serotonin release in the preoptic area and anterior hypothalamus is not involved in thermoregulation during low-intensity exercise in a warm environment", Neuroscience Letters, 482(1), pp.7~11 (2010)
  41. Tsai, C. F., "The neuroprotective effects of an extract of Gastrodia elata", Journal of Ethnopharmacology, 138(1), pp.119~125 (2011)
  42. Tsai, L. H., "p35 is a neural-specific regulatory subunit of cyclin-dependent kinase 5", Nature, 371(6496), pp.419~423 (1994)
  43. Tuan, T. C., "Deleterious effects of short-term, high-intensity exerciseon immune function : evidence from leucocyte mitochondrial alterations and apoptosis", British Journal of Sports Medicine, 42(1), pp.11~15 (2008)
  44. van Praag, H., "Running enhances neurogenesis, learning, and long-term potentiation in mice", Proceedings of the National Academy of Sciences of the United States of America, 96(23), pp.13427~13431 (1999)
  45. Vinay, L., "Plasticity of spinal cord locomotor networks and contribution of cation-chloride cotransporters", Brain Research Reviews, 57(1), pp.103~110 (2008)
  46. Yu, S. J., "Gastrodia elata blume and an active component, p-hydroxybenzyl alcohol reduce focal ischemic brain injury through antioxidant related gene expressions", Biological & Pharmaceutical Bulletin, 28(6), pp.1016~1020 (2005)
  47. Yuen, A. W., "Vitamin D : in the evolution of human skin colour", Medical Hypotheses, 74(1), pp.39~44 (2010)

관련 논문AI추천

트레드밀 운동과 밝은 빛이 흰쥐 뇌의 세로토닌 발현에 미치는 영향권선옥, 권성진, 송광섭국민체육진흥공단 한국스포츠과학원 · 체육과학연구2014 · 제25권 제2호Treadmill Exercise Improves Memory Function Depending on Circadian Rhythm Changes in Mice고일규, 곽효범, 권오영, 김성은, 지은상, 진준장, 최현희, 황동섭대한배뇨장애요실금학회 · International Neurourology Journal2016 · 제20권 제4호규칙적인 운동이 성인 백서 해마 치상회에서 신경세포의 Dendritic Lengths 및 SynapsinⅠ 수준에 미치는 영향김현태, 이효철국민체육진흥공단 한국스포츠과학원 · 체육과학연구2012 · 제23권 제4호뇌 타박 손상 후 해마에서 신경세포 생성 및 신경가소성 조절 단백질 발현에 미치는 운동 기간에 따른 효과백일영, 서태범, 윤진환, 장문녀국민체육진흥공단 한국스포츠과학원 · 체육과학연구2011 · 제22권 제3호Treadmill Exercise Alleviates Circadian Rhythm Disruption- Induced Memory Deficits by Activation of Glucocorticoid Receptor and Brain-Derived Neurotrophic Factor-Dependent Pathway고일규, 김경진, 김계환, 김상훈, 김성은, 김창주, 조세형, 지은상, 진준장, 황락경대한배뇨장애요실금학회 · International Neurourology Journal2019 · 제23권 제1호노화 쥐 해마 CA1에서의 운동을 통한 BDNF 발현 유도 및 PI3K/AKT에 의한 synaptic 단백질 발현김동문, 어수주, 임예현한국운동생리학회 · 운동과학2012 · 제21권 제1호

이 간행물의 다른 논문 (13건)