Comparative Analysis of Dibutyric cAMP and Butyric Acid on the Differentiation of Human Eosinophilic Leukemia EoL-1 Cells
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Purification of enough numbers of circulating eosinophils is difficult because eosinophils account for less than 5% peripheral blood leukocytes. Human eosinophilic leukemia EoL-1 cells have been considered an in vitro source of eosinophils as they can differentiate into mature eosinophillike cells when incubated with dibutyryl cAMP (dbcAMP) or butyric acid. In this study, the viability and phenotypic maturation of EoL-1 cells stimulated by either dbcAMP or butyric acid were comparatively analyzed. After treatment with 100 μM dbcAMP or 0.5 μM butyric acid, EoL-1 cells showed morphological signs of differentiation, although the number of nonviable EoL-1 cells was significantly increased following butyric acid treatment. Stimulation of EoL-1 cells with 0.5 μM butyric acid more effectively induced the expression of mature eosinophil markers than stimulation with dbcAMP. These results suggest that treatment of EoL-1 cells with 0.5 μM butyric acid for limited duration could be an effective strategy for inducing their differentiation. Considering that expression of CCR3 was not sufficient in EoL-1 cells stimulated with 0.5 μM butyric acid, treatment of the chemically stimulated EoL-1 cells with cytokines, which primarily support eosinophil maturation, would help to obtain differentiated EoL-1 cells with greater functional maturity.
Abstract
Purification of enough numbers of circulating eosinophils is difficult because eosinophils account for less than 5% peripheral blood leukocytes. Human eosinophilic leukemia EoL-1 cells have been considered an in vitro source of eosinophils as they can differentiate into mature eosinophillike cells when incubated with dibutyryl cAMP (dbcAMP) or butyric acid. In this study, the viability and phenotypic maturation of EoL-1 cells stimulated by either dbcAMP or butyric acid were comparatively analyzed. After treatment with 100 μM dbcAMP or 0.5 μM butyric acid, EoL-1 cells showed morphological signs of differentiation, although the number of nonviable EoL-1 cells was significantly increased following butyric acid treatment. Stimulation of EoL-1 cells with 0.5 μM butyric acid more effectively induced the expression of mature eosinophil markers than stimulation with dbcAMP. These results suggest that treatment of EoL-1 cells with 0.5 μM butyric acid for limited duration could be an effective strategy for inducing their differentiation. Considering that expression of CCR3 was not sufficient in EoL-1 cells stimulated with 0.5 μM butyric acid, treatment of the chemically stimulated EoL-1 cells with cytokines, which primarily support eosinophil maturation, would help to obtain differentiated EoL-1 cells with greater functional maturity.
참고문헌 (17)
- Rothenberg, M. E., "The eosinophil", Annu. Rev. Immunol, 24, pp.147~174 (2006)
- Rothenberg, M. E., "Eosinophilic gastrointestinal disorders (EGID)", J. Allergy Clin. Immunol, 113, pp.11~28 (2004)
- Milanovic, M., "IFN consensus sequence binding protein (Icsbp)is critical for eosinophil development", J. Immunol, 181, pp.5045~5053 (2008)
- Hogan, S. P., "Eosinophils in infection intestinal immunity", Curr. Opin. Gastroenterol, 29, pp.7~14 (2013)
- Jung, Y., "Roles regulation of gastrointestinal eosinophils in immunity disease", J. Immunol, 193, pp.999~1005 (2014)
- Jung, Y. J., "Human eosinophils show chemotaxis to lymphoid chemokines exhibit antigen-presenting-cell-like properties upon stimulation with IFN-gamma, IL-3GM-CSF", Int. Arch. Allergy Immunol, 146, pp.227~234 (2008)
- Saito, H., "Establishment characterization of a new human eosinophilic leukemia cell line", Blood, 66, pp.1233~1240 (1985)
- Mayumi, M., "EoL-1, a human eosinophilic cell line", Leuk. Lymphoma, 7, pp.243~250 (1992)
- Wong, C. K., "Differentiation of a human eosinophilic leukemic cell line, EoL-1: characterization by the expression of cytokine receptors, adhesion molecules, CD95 eosinophilic cationic protein (ECP)", Immunol. Lett, 68, pp.317~323 (1999)
- Yoshida, T., "Defective B-1 cell development impaired immunity against Angiostrongylus cantonensis in IL-5R alpha-deficient mice", Immunity, 4, pp.483~494 (1996)
- Kitaura, M., "Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3", J. Biol. Chem, 271, pp.7725~7730 (1996)
- Ishihara, K., "Differentiation of eosinophilic leukemia EoL-1 cells into eosinophils induced by histone deacetylase inhibitors", Life Sci, 80, pp.1213~1220 (2007)
- Saito, H., "Effect of butyric acid on induction of differentiation into eosinophil-like cells in human eosinophilic leukemia cells, EoL-1 cell line: possible role of granulocyte-macrophage colony-stimulating factor as an autocrine differentiating factor", Int. Arch. Allergy Immunol, 100, pp.240~247 (1993)
- Morita, M., "Differentiation of a human eosinophilic leukemia cell line(EoL-1)by a human T-cell leukemia cell line(HIL-3)-derived factor", Blood, 77, pp.1766~1775 (1991)
- Iwasaki, H., "Identification of eosinophil lineage-committed progenitors in the murine bone marrow", J. Exp. Med, 201, pp.1891~1897 (2005)
- Clutterbuck, E. J., "Human interleukin-5(IL-5)regulates the production of eosinophils in human bone marrow cultures : comparison interaction with IL-1, IL-3, IL-6, GMCSF", Blood, 73, pp.1504~1512 (1989)
- Fulkerson, P. C., "IL-5 triggers a cooperative cytokine network that promotes eosinophil precursor maturation", J. Immunol, 193, pp.4043~4052 (2014)