The Effect of either Aerobic Exercise Training or Chrysin Supplementation on Mitochondrial Biogenesis in Skeletal Muscle of High Fat Diet-Induced Obese Mice
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
PURPOSE: The purpose of this study was to investigate the effects of either aerobic exercise or resveratrol supplementation on mitochondrial biogenesis in skeletal muscle of the high fat diet-induced obese mice. METHODS: C57BL/6 (4 weeks aged) male mice were divided into four groups; normal diet group (NC, n=10), high fat diet group (HC), high fat diet group with chrysin supplementation (HCh, n=10) and high fat diet with exercise group (HE, n=10). Aerobic exercise was performed on a treadmill exercise for 40-60 min/day at 10-14 m/min, 0% grade, 4 days/week for 16 weeks. Chrysin (50 mg/ kg body weight) daily administrated orally at 4 days/week frequency for 16 weeks. RESULTS: The gene expression of SIRT-3 in HE group was significantly increased compared to that in HC group (p<0.05). Also, PGC-1α and COX-IV mRNA levels in the HE group were the highest among all groups (p<0.05). CONCLUSIONS: It seems that high fat diet-induced obese dose not affect mitochondrial biogenesis gene expression in skeletal muscle. However aerobic exercise training could increase the mitochondrial biogenesis gene expression of skeletal muscle in high fat dietinduced obese mice. These findings suggest that only aerobic exercise not chrysin supplementation has a positive effect on mitochondrial biogenesis in the skeletal muscle in high fat diet-induced obese mice.
Abstract
PURPOSE: The purpose of this study was to investigate the effects of either aerobic exercise or resveratrol supplementation on mitochondrial biogenesis in skeletal muscle of the high fat diet-induced obese mice. METHODS: C57BL/6 (4 weeks aged) male mice were divided into four groups; normal diet group (NC, n=10), high fat diet group (HC), high fat diet group with chrysin supplementation (HCh, n=10) and high fat diet with exercise group (HE, n=10). Aerobic exercise was performed on a treadmill exercise for 40-60 min/day at 10-14 m/min, 0% grade, 4 days/week for 16 weeks. Chrysin (50 mg/ kg body weight) daily administrated orally at 4 days/week frequency for 16 weeks. RESULTS: The gene expression of SIRT-3 in HE group was significantly increased compared to that in HC group (p<0.05). Also, PGC-1α and COX-IV mRNA levels in the HE group were the highest among all groups (p<0.05). CONCLUSIONS: It seems that high fat diet-induced obese dose not affect mitochondrial biogenesis gene expression in skeletal muscle. However aerobic exercise training could increase the mitochondrial biogenesis gene expression of skeletal muscle in high fat dietinduced obese mice. These findings suggest that only aerobic exercise not chrysin supplementation has a positive effect on mitochondrial biogenesis in the skeletal muscle in high fat diet-induced obese mice.
참고문헌 (52)
- Borg ML, "Consumption of a high-fat diet, but not regular endurance exercise training, regulates hypothalamic lipid accumulation in mice", J Physiol, 590(17), pp.4377~4389 (2012)
- 박희근, "Exercise training is more effective than resveratrol supplementation on alleviation of inflammation in peritoneal macrophages of high fat diet mice", Physical Activity and Nutrition, 18(1), pp.79~87 (2014), 한국운동영양학회
- Patti ME, "Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes : Potential role of PGC1 and NRF1", PNAS, 100(14), pp.8466~8471 (2003)
- Wilson FH, "A cluster of metabolic defects caused by mutation in a mitochondrial tRNA", Science, 306(5699), pp.1190~1194 (2004)
- Lowell BB, "Mitochondrial dysfunction and type 2 diabetes", Science, 307(5708), pp.384~387 (2005)
- Johnson ML, "Skeletal muscle aging and the mitochondrion", Trends Endocrinol Metab, 24(5), pp.247~256 (2013)
- Lin J, "Metabolic control through the PGC-1 family of transcription coactivators", Cell Metab, 1(6), pp.361~370 (2005)
- Miura S, "Overexpression of peroxisome proliferator-activated receptor γ co-activator-1αleads to muscle atrophy with depletion of ATP", Am J Pathol, 169(4), pp.1129~1139 (2006)
- Wende AR, "A role for the transcriptional coactivator PGC-1α in muscle refueling", J Biol Chem, 282(50), pp.36642~36651 (2007)
- Hood DA, "Mechanisms of exercise-induced mitochondrial biogenesis in skeletal muscle", Appl Physiol Nutr Metab, 34(3), pp.465~472 (2009)
- Irrcher I, "PPARγcoactivator-1α expression during thyroid hormone-and contractile activity-induced mitochondrial adaptations", Am J Physiol Cell Physiol, 284(6), pp.C1669~C1677 (2003)
- Chabi B, "Mitochondrial function and apoptotic susceptibility in aging skeletal muscle", Aging Cell, 7(1), pp.2~12 (2008)
- Palacios OM, "Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1αin skeletal muscle", Aging Cell, 1(9), pp.771~ (2009)
- Hokari F, "Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles", J Appl Physiol, 109(2), pp.332~340 (2010)
- Lanza IR, "Endurance exercise as a countermeasure for aging", Diabetes, 57(11), pp.2933~2942 (2008)
- Burgomaster KA, "Divergent response of metabolite transport proteins in human skeletal muscle after sprint interval training and detraining", Am J Physiol Regul Integr Comp Physiol, 292(5), pp.1970~1976 (2007)
- Geng T, "PGC-1α plays a functional role in exercise-induced mitochondrial biogenesis and angiogenesis but not fiber-type transformation in mouse skeletal muscle", Am J Physiol Cell Physiol, 298(3), pp.572~579 (2009)
- Short KR, "Impact of aerobic exercise training on age-related changes in insulin sensitivity and muscle oxidative capacity", Diabetes, 52(8), pp.1888~1896 (2003)
- Olesen J, "PGC-1α-mediated adaptations in skeletal muscle", Pflugers Arch, 460(1), pp.153~162 (2010)
- Manach C, "Polyphenols : food sources and bioavailability", Am J Clin Nutr, 79(5), pp.727~747 (2004)
- Rapta P, "Redox intermediates of flavonoids and caffeic acid esters from propolis : an EPR spectroscopy and cyclic voltammetry study", Free Radic Biol Med, 18(5), pp.901~908 (1995)
- Williams CA, "Chrysin and other leaf exudate flavonoids in the genus Pelargonium", Phytochemistry, 46(8), pp.1349~1353 (1997)
- Khan R, "Chrysin protects against cisplatin-induced colon. toxicity via amelioration of oxidative stress and apoptosis: probable role of p38MAPK and p53", Toxicol Appl Pharmacol, 258(3), pp.315~329 (2012)
- Cherkaoui Tangi K, "Pharmacological studies on hypotensive, diuretic and vasodilator activities of chrysin glucoside from Calycotome villosa in rats", Phytother Res, 22(3), pp.356~361 (2008)
- Mi-Young Song, "Sulfuretin protects against cytokine-induced β-cell damage and prevents streptozotocin-induced diabetes", Experimental and Molecular Medicine, 42(9), pp.628~638 (2010), 생화학분자생물학회
- Tahir M, "Chrysin modulates ethanol metabolism in Wistar rats : a promising role against organ toxicities", Alcohol Alcohol, 46(4), pp.383~392 (2011)
- Pushpavalli G, "Influence of chrysin on hepatic marker enzymes and lipid profile against D-galactosamine-induced hepatotoxicity rats", Food Chem Toxicol, 48(6), pp.1654~1659 (2010)
- Schefer V, "Oxygen consumption in adult and AGED C57BL", 6J mice during acute treadmill exercise of different intensity, 1996, pp.31(3), 387~392
- King GA, "Relationship of leisure-time physical activity and occupational activity to the prevalence of obesity", Int J Obes, 25(5), pp.606~ (2001)
- Kandhare AD, "Evaluation of the neuroprotective effect of chrysin via modulation of endogenous biomarkers in a rat model of spinal cord injury", J Nat Med, 68(3), pp.586~603 (2014)
- Feng X, "Chrysin attenuates inflammation by regulating M1", M2 status via activating PPARγ, 2014, pp.89(4), 503~514
- Baur JA, "Biochemical effects of SIRT1 activators", Biochim. Biochim Biophys Acta, 1804(8), pp.1626~1634 (2010)
- Lin J, "Transcriptional co-activator PGC-1α drives the formation of slow-twitch muscle fibres", Nature, 418(6899), pp.797~ (2002)
- Lagouge M, "Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1α", Cell, 127(6), pp.1109~1122 (2006)
- Lopez-Lluch G, "Calorie restriction induces mitochondrial biogenesis and bioenergetic efficiency", PNAS, 103(6), pp.1768~1773 (2006)
- Choi JH, "Chrysin induces brown fat-like phenotype and enhances lipid metabolism in 3T3-L1 adipocytes", Nutrition, 32(9), pp.1002~1010 (2016)
- Lombard DB, "Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation", Mol Cell Biol, 27(24), pp.8807~8814 (2007)
- North BJ, "Sirtuins : a conserved key unlocking AceCS activity", Trends Biochem Sci, 32(1), pp.1~4 (2007)
- Hallows WC, "Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases", PNAS, 103(27), pp.10230~10235 (2006)
- Hirschey MD, "SIRT3deficiency and mitochondrial protein hyperacetylation accelerate the development of the metabolic syndrome", Mol Cell, 44(2), pp.177~190 (2011)
- Kong X, "Sirtuin 3, a new target of PGC-1α, plays an important role in the suppression of ROS and mitochondrial biogenesis", PLoS One, 5(7), pp.e11707~ (2010)
- Gurd BJ, "In mammalian muscle, SIRT3 is present in mitochondria and not in the nucleus; and SIRT3 is upregulated by chronic muscle contraction in an adenosine monophosphate-activated protein kinase–independent manner", Metabolism, 61(5), pp.733~741 (2012)
- Jelenik T, "Mitochondrial plasticity in obesity and diabetes mellitus", Antioxid Redox Signal, 19(3), pp.258~268 (2013)
- Mensink M, "Improved skeletal muscle oxidative enzyme activity and restoration of PGC-1αand PPARβ", δ gene expression upon rosiglitazone treatment in obese patients with type 2 diabetes mellitus, 2007, pp.31(8), 1302~
- Samocha Bonet D, "Metabolically healthy and unhealthy obese–the 2013 Stock Conference report", Obes Rev, 15(9), pp.697~708 (2014)
- Laker RC, "Exercise prevents maternal high-fat diet–induced hypermethylation of the Pgc-1α gene and age-dependent metabolic dysfunction in the offspring", Diabetes, 63(5), pp.1605~1611 (2014)
- Handschin C, "Peroxisome proliferator-activated receptor γ coactivator 1 coactivators, energy homeostasis, and metabolism", Endocr Rev, 27(7), pp.728~735 (2006)
- Handschin C, "Abnormal glucose homeostasis in skeletal muscle–specific PGC-1α knockout mice reveals skeletal muscle–pancreatic β cell crosstalk", J Clin Inves, 117(11), pp.3463~3474 (2007)
- Cartoni R, "Mitofusins 1", 2 and ERRα expression are increased in human skeletal muscle after physical exercise, 2005, pp.567(1), 349~358
- Cobley JN, "PGC-1α transcriptional response and mitochondrial adaptation to acute exercise is maintained in skeletal muscle of sedentary elderly males", Biogerontology, 13(6), pp.621~631 (2012)
- Greene NP, "Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight", obese men and women, 2012, pp.303(10), 1212~1221
- Scarpulla RC, "Metabolic control of mitochondrial biogenesis through the PGC-1 family regulatory network", Biochim Biophys Acta, 1813(7), pp.1269~1278 (2011)