본문 바로가기
KERD
논문KCI 등재

Effects of Exercise Sequence and Circadian Rhythms on Molecular Mechanisms of Muscle Hypertrophy and Mitochondrial Biogenesis in Obese Rat

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

PURPOSE: This study aimed to investigate whether endurance and resistance training sequences and circadian rhythms affect muscle hypertrophy and mitochondrial biogenesis-related molecules in obese rats. METHODS: Rats were randomly divided into five groups: the obesity control group (OCG), aerobic-resistance exercise in the morning group (ARMG), resistance-aerobic exercise in the morning group (RAMG), aerobic resistance exercise in the evening group (AREG), and resistance-aerobic exercise in the evening group (RAEG). The exercise groups performed endurance and resistance exercises for 8 weeks according to their circadian rhythms and exercise sequences. RESULTS: We evaluated specific muscle hypertrophy and mitochondrial biogenesis markers in the flexor pollicis longus and soleus muscles using western blot and immunofluorescence techniques. The body weight was significantly decreased in the ARMG, RAMG, AREG, RAEG compared to the OCG after intervention. The soleus muscle fiber cross-sectional area and muscle hypertrophy markers p-Akt and p-mTOR were significantly increased in the AREG group compared with those in the OCG, ARMG, RAMG, and RAEG groups after exercise intervention. Most mitochondrial biogenesis-related markers were significantly increased in the RAMG group than in the other groups after exercise intervention. CONCLUSION: Our findings provide new evidence that muscle hypertrophy might be upregulated by resistance exercise after evening endurance exercise. In addition, morning resistance exercise followed by aerobic exercise, might promote mitochondrial biogenesis.

Abstract

PURPOSE: This study aimed to investigate whether endurance and resistance training sequences and circadian rhythms affect muscle hypertrophy and mitochondrial biogenesis-related molecules in obese rats. METHODS: Rats were randomly divided into five groups: the obesity control group (OCG), aerobic-resistance exercise in the morning group (ARMG), resistance-aerobic exercise in the morning group (RAMG), aerobic resistance exercise in the evening group (AREG), and resistance-aerobic exercise in the evening group (RAEG). The exercise groups performed endurance and resistance exercises for 8 weeks according to their circadian rhythms and exercise sequences. RESULTS: We evaluated specific muscle hypertrophy and mitochondrial biogenesis markers in the flexor pollicis longus and soleus muscles using western blot and immunofluorescence techniques. The body weight was significantly decreased in the ARMG, RAMG, AREG, RAEG compared to the OCG after intervention. The soleus muscle fiber cross-sectional area and muscle hypertrophy markers p-Akt and p-mTOR were significantly increased in the AREG group compared with those in the OCG, ARMG, RAMG, and RAEG groups after exercise intervention. Most mitochondrial biogenesis-related markers were significantly increased in the RAMG group than in the other groups after exercise intervention. CONCLUSION: Our findings provide new evidence that muscle hypertrophy might be upregulated by resistance exercise after evening endurance exercise. In addition, morning resistance exercise followed by aerobic exercise, might promote mitochondrial biogenesis.

참고문헌 (37)

  1. Visscher TL, "The public health impact of obesity", Annu Rev Public Health, 22(1), pp.355~375 (2001)
  2. Speakman JR, "Physical activity and resting metabolic rate", Proc Nutr Soc, 62(3), pp.621~634 (2003)
  3. Liang H, "PGC-1α : a key regulator of energy metabolism", Adv Physiol Educ (2006)
  4. Coffey VG, "The molecular bases of training adaptation", Sports Med, 37, pp.737~763 (2007)
  5. Bodine SC, "mTOR signaling and the molecular adaptation to resistance exercise", Med Sci Sports Exerc, 38(11), pp.1950~1957 (2006)
  6. Atherton PJ, "Selective activation of AMPK-PGC-1α or PKB-TSC2-mTOR signaling can explain specific adaptive responses to endurance or resistance traininglike electrical muscle stimulation", FASEB J, 19(7), pp.1~23 (2005)
  7. Ogasawara R, "The order of concurrent endurance and resistance exercise modifies mTOR signaling and protein synthesis in rat skeletal muscle", Am J Physiol Endocrinol Metab, 306(10), pp.E1155~E62 (2014)
  8. Han H, "Role of circadian rhythm and impact of circadian rhythm disturbance on the metabolism and disease", J Cardiovasc Pharmacol, 79(3), pp.254~263 (2022)
  9. Woodie LN, "The circadian regulation of nutrient metabolism in diet-induced obesity and metabolic disease", Nutrients, 14(15), pp.3136~ (2022)
  10. Engin A, "Circadian rhythms in diet-induced obesity", Adv Exp Med Biol, pp.19~52 (2017)
  11. Serin Y, "Effect of circadian rhythm on metabolic processes and the regulation of energy balance", Ann Nutr Metab, 74(4), pp.322~330 (2019)
  12. Kim HK, "Effects of acute endurance exercise performed in the morning and evening on inflammatory cytokine and metabolic hormone responses", PLoS One, 10(9), pp.e0137567~ (2015)
  13. Küüsmaa M, "Effects of morning versus evening combined strength and endurance training on physical performance, muscle hypertrophy, and serum hormone concentrations", Appl Physiol Nutr Metab, 41(12), pp.1285~1294 (2016)
  14. Kim HK, "Effects of different intensities of endurance exercise in morning and evening on the lipid metabolism response", J Sports Sci Med, 15(3), pp.467~ (2016)
  15. Liao J, "Dysfunction of perivascular adipose tissue in mesenteric artery is restored by aerobic exercise in high-fat diet induced obesity", Clin Exp Pharmacol Physiol, 48(5), pp.697~703 (2021)
  16. Zhang YJ, "Effect of electroacupuncture combined with treadmill exercise on body weight and expression of PGC-1α, Irisin and AMPK in skeletal muscle of diet-induced obesity rats", Zhen Ci Yan Jiu, 44(7), pp.476~480 (2019)
  17. Cassilhas RC, "Resistance exercise improves hippocampus-dependent memory", Braz J Med Biol Res, 45, pp.1215~1220 (2012)
  18. Kraemer WJ, "Resistance exercise induces region-specific adaptations in anterior pituitary gland structure and function in rats", J Appl Physiol, 115(11), pp.1641~1647 (2013)
  19. Abdelmoula A, "Knee extension strength in obese and nonobese male adolescents", Appl Physiol Nutr Metab, 37(2), pp.269~275 (2012)
  20. Heinonen S, "Impaired mitochondrial biogenesis in adipose tissue in acquired obesity", Diabetes, 64(9), pp.3135~3145 (2015)
  21. Froy O, "Metabolism and circadian rhythms-implications for obesity", Endocr Rev, 31(1), pp.1~24 (2010)
  22. Mohebbi H, "Maximal fat oxidation at the different exercise intensity in obese and normal weight men in the morning and evening", J Sport Exerc Psychol, 6(1), pp.49~58 (2011)
  23. Alizadeh Z, "Comparison between the effect of 6 weeks of morning or evening aerobic exercise on appetite and anthropometric indices : a randomized controlled trial", Clin Obes, 7(3), pp.157~165 (2017)
  24. Chatterjee S, "Circadian clock regulation of skeletal muscle growth and repair", F1000Res, 5, pp.1549~ (2016)
  25. Bodine SC, "mTOR signaling and the molecular adaptation to resistance exercise", Med Sci Sports Exerc, 38(11), pp.1950~1957 (2006)
  26. Sedliak M, "Effects of time of day on resistance exercise-induced anabolic signaling in skeletal muscle", Biol Rhythm Res, 44(5), pp.756~770 (2013)
  27. Burley SD, "The differential hormonal milieu of morning versus evening may have an impact on muscle hypertrophic potential", PLoS One, 11(9), pp.e0161500~ (2016)
  28. Ezagouri S, "Physiological and molecular dissection of daily variance in exercise capacity", Cell Metab, 30(1), pp.78~91 (2019)
  29. Perez-Schindler J, "Nutritional strategies to support concurrent training", Eur J Sport Sci, 15(1), pp.41~52 (2015)
  30. Dreyer HC, "Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle", J Physiol, 576(2), pp.613~624 (2006)
  31. Frøsig C, "5ʼ-AMP-activated protein kinase activity and protein expression are regulated by endurance training in human skeletal muscle", Am J Physiol Endocrinol Metab, 286(3), pp.E411~E417 (2004)
  32. Nielsen JN, "5ʼ-AMP-activated protein kinase activity and subunit expression in exercise-trained human skeletal muscle", Journal of J Appl Physiol, 94(2), pp.631~641 (2003)
  33. Rose AJ, "Effect of endurance exercise training on Ca2+calmodulin-dependent protein kinase II expression and signalling in skeletal muscle of humans", J Physiol, 583(Pt 2), pp.785~795 (2007)
  34. Egan B, "Exercise intensitydependent regulation of peroxisome proliferator-activated receptor γ coactivator-1α mRNA abundance is associated with differential activation of upstream signalling kinases in human skeletal muscle", J Physiol, 588(10), pp.1779~1790 (2010)
  35. Brandt N, "PGC-1alpha and exercise intensity dependent adaptations in mouse skeletal muscle", PLoS One, 12(10), pp.e0185993~ (2017)
  36. 김기훈, "Effect of Exercise Intensity on Unfolded Protein Response in Skeletal Muscle of Rat", The Korean Journal of Physiology & Pharmacology, 18(3), pp.211~216 (2014), 대한약리학회
  37. de Goede P, "Circadian rhythms in mitochondrial respiration", J Mol Endocrinol, 60(3), pp.R115~R130 (2018)

관련 논문AI추천

Effects of Exercise Intensity during the Detraining Period on Changed Muscle Hypertrophy- and Mitochondrial Biogenesis-Related Proteins after High-Intensity Interval Training서태범, 유주인, 조용현국민체육진흥공단 한국스포츠과학원 · IJASS(International Journal of Applied Sports Sciences)2023 · 제35권 제2호The Effect of either Aerobic Exercise Training or Chrysin Supplementation on Mitochondrial Biogenesis in Skeletal Muscle of High Fat Diet-Induced Obese Mice김경일, 박희근, 안상민, 이광무, 이왕록한국운동생리학회 · 운동과학2019 · 제28권 제4호Effects of aerobic exercise on the expressions of skeletal muscle fiber type-related genes and FNDC-5 in high-fat diet-induced obese rats한진, 서대윤, 여지영, 전유나, 최승준, 하경진국민체육진흥공단 한국스포츠과학원 · IJASS(International Journal of Applied Sports Sciences)2017 · 제29권 제1호Association of VEGF protein expression according to muscle fiber types with diet and exercise intervention in diet-induced obese rats김기진, 천우광, 홍창배한국운동생리학회 · 운동과학2013 · 제22권 제1호Exercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle강주희, 곽효범, 김지연, 박동호, 박은미, 배준현, 서대윤, 윤진환, 이석호, 조은정, 조진경, 최영주대한배뇨장애요실금학회 · International Neurourology Journal2021 · 제25권 제1호Effects of Exercise Training on Vascular Inflammatory Markers in High-fat Diet-induced Obese Rats김기진대한운동학회 · 아시아 운동학 학술지2020 · 제22권 제2호

이 간행물의 다른 논문 (13건)