Diets with corn oil and/or low protein increase acute acetaminophen hepatotoxicity compared to diets with beef tallow in a rat model
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It has been reported that dietary polyunsaturated fats (PUFA) increase liver injury in response to ethanol feeding. We tested the hypothesis that diets rich in linoleic acid (18:2n-6) would affect acute liver injury after acetaminophen injection and that protein restriction might exacerbate the liver injury. We examined effects of feeding diets with either 15% (wt/wt) corn oil or 14% beef tallow and 1% corn oil for six weeks with either 6 or 20 g/100 g protein on acute hepatotoxicity. After the feeding period, liver injury was induced by injecting either with 600 mg/kg body weight acetaminophen suspended in gum arabic-based vehicle, or with vehicle alone during fasting status. Samples of liver and plasma were taken for analyses of hepatic glutathione (GSH) levels and liver-specific enzymes [(Glutamate-pyruvate transaminase (GPT) and glutamate-oxaloacetate transaminase (GOT)], respectively. Whereas GSH level was significantly lower in only group fed 15% corn oil with 6 g/100 g protein among acetaminophen-treated groups, activities of GPT and GOT were significantly elevated in all groups except the one fed beef tallow with 20 g/100 g protein, suggesting low protein might exacerbate drug-induced hepatotoxicity. The feeding regimens changed the ratio of 18:2n-6 to oleic acid (18:1n-9) in total liver lipids approximately five-fold, and produced modest changes in arachidonic acid (20:4n-6). We conclude that diets with high 18:2n-6 promote acetaminophen-induced liver injury compared to diets with more saturated fatty acids (SFA). In addition, protein restriction appeared to exacerbate the liver injury.
Abstract
It has been reported that dietary polyunsaturated fats (PUFA) increase liver injury in response to ethanol feeding. We tested the hypothesis that diets rich in linoleic acid (18:2n-6) would affect acute liver injury after acetaminophen injection and that protein restriction might exacerbate the liver injury. We examined effects of feeding diets with either 15% (wt/wt) corn oil or 14% beef tallow and 1% corn oil for six weeks with either 6 or 20 g/100 g protein on acute hepatotoxicity. After the feeding period, liver injury was induced by injecting either with 600 mg/kg body weight acetaminophen suspended in gum arabic-based vehicle, or with vehicle alone during fasting status. Samples of liver and plasma were taken for analyses of hepatic glutathione (GSH) levels and liver-specific enzymes [(Glutamate-pyruvate transaminase (GPT) and glutamate-oxaloacetate transaminase (GOT)], respectively. Whereas GSH level was significantly lower in only group fed 15% corn oil with 6 g/100 g protein among acetaminophen-treated groups, activities of GPT and GOT were significantly elevated in all groups except the one fed beef tallow with 20 g/100 g protein, suggesting low protein might exacerbate drug-induced hepatotoxicity. The feeding regimens changed the ratio of 18:2n-6 to oleic acid (18:1n-9) in total liver lipids approximately five-fold, and produced modest changes in arachidonic acid (20:4n-6). We conclude that diets with high 18:2n-6 promote acetaminophen-induced liver injury compared to diets with more saturated fatty acids (SFA). In addition, protein restriction appeared to exacerbate the liver injury.
참고문헌 (39)
- Alexander-North LS, "Polyunsaturated fatty acids increase lipid radical formation induced by oxidant stress in endothelial cells", J Lipid Res, 35, pp.1773~1785 (1994)
- AOAC, "Official Methods of Analysis, 14th Edition", Association of Official Analytical Chemists, ~ (1984)
- Baumgardner JN, "A new model for nonalcoholic steatohepatitis in the rat utilizing total enteral nutrition to overfeed a high-polyunsaturated fat diet", Am J Physiol Gastrointest Liver Physiol, 294, pp.G27~38 (2008)
- Buller KL, "The effects of food intake and dietary fatty acids on the activity of stearoyl-CoA Δ9-desaturase in pig adipose tissue", J Agri Sci, 106, pp.601~609 (1986)
- Camara M, "Influence of two dietary fats on lipid synthesis in the pig: Comparative study of liver, muscle and two back fat layers", Ann Nutr Metab, 40, pp.287~295 (1996)
- Deneke SM, "Effects of low protein diets or feed restriction on rat lung glutathione and oxygen toxicity", J Nutr, 115, pp.726~732 (1985)
- Ervin RB, "Dietary intake of fats and fatty acids for the United states population:1999-2000", Adv Data, 348, pp.1~6 (2004)
- Folch J, "Extraction of fatty acid", J Biol Chem, 226, pp.497~509 (1957)
- Frederiks WM, "A method for quantitative analysis of the extent of necrosis ischemic rat liver", Exp Mol Pathol, 41, pp.119~125 (1984)
- French SM, "Lipid peroxidation, CYP 2E1 and arachidonic acid metabolism in alcoholic liver disease in rats", J Nutr, 127, pp.907S~911S (1997)
- Gonzalez FJ, "Role of cytochromes P450 in chemical toxicity and oxidative stress: studies with CYP2E1", Mutat Res, 569, pp.101~110 (2005)
- Guarner F, "Reduction by prostacyclin of acetaminophen-induced liver toxicity in the mouse", Hepatology, 8, pp.248~253 (1988)
- Hennig B, "Fatty acid-mediated effects on the glutathione redox cycle in cultured endothelial cells", Am J Clin Nutr, 59, pp.60~65 (1994)
- Hennig B, "Protection by vitamin E against endothelial cell injury by linoleic acid hydroperoxides", Nutr Res, 7, pp.1253~1259 (1987)
- Huang CJ, "Protein insufficiency aggravates the enhanced lipid peroxidation and reduced activities of antioxidant enzymes in rats fed diets high in polyunsaturated fat", J Nutr, 122, pp.1182~1189 (1992)
- Ito Y, "Dietary steatotic liver attenuates acetaminophen hepatotoxicity in mice", Microcirculation, 13, pp.19~27 (2006)
- Jaya DS, "Role of lipid peroxides, glutathione and antiperoxidative enzymes in alcohol and drug toxicity", Ind J Exp Bio, 31, pp.453~459 (1993)
- Kuralay F, "Possible role of glutathione in prevention of acetaminopheninduced hepatotoxicity enhanced by fish oil in male Wistar rats", J Toxicol Environ Health A, 53, pp.223~229 (1998)
- Lee C, "Liposomes enriched in oleic acid are less susceptible to oxidation and have less proinflammatory activity when exposed to oxidizing conditions", J Lipid Res, 39, pp.1239~1247 (1998)
- Lim SP, "Misoprostol protection against acetaminophen-induced hepatotoxicity in the rat", Dig Dis Sci, 39, pp.1249~1256 (1994)
- McDanell RE, "Effect of dietary fat on the in vitro hepatotoxicity of paracetamol", Biochem Pharmacol, 44, pp.1303~1306 (1992)
- Momchilova A, "Sensitivity of 5’-nucleotidase and phospholipase A2 towards liver plasma membrane modifications", Int J Biochem, 17, pp.787~792 (1985)
- Moussa M, "Relationship between the fatty acid composition of rat lymphocytes and immune functions", Br J Nutr, 83, pp.327~333 (2000)
- Nakamura MT, "Selective reduction of Δ6 and Δ5 desaturase activities but not Δ9 desaturase in micropigs chronically fed ethanol", J Clin Invest, 93, pp.450~454 (1994)
- Nanji AA, "Liver microsomal fatty acid composition in ethanol-fed rats: effect of different dietary fats and relationship to liver injury", J Nutr, 18, pp.1024~1028 (1994)
- Nanji AA, "Dietary saturated fatty acids: a novel treatment for alcoholic liver disease", Gastroenterology, 109, pp.547~554 (1995)
- Nanji AA, "Decreased prostacyclin production by liver non-parenchymal cells precedes liver injury in experimental alcoholic liver disease", Life Sci, 54, pp.455~461 (1994)
- Nanji, AA, "Beef fat prevents alcoholic liver disease in the rat", Alcohol Clin Exp Res, 13, pp.15~19 (1989)
- Nanji AA, "Enhanced cyclooxygenase-2 gene expression in alcoholic liver disease in the rat", Gastroenterology, 112, pp.943~951 (1997), .
- Nanji AA, "Changes in microsomal phospholipases and arachidonic acid in experimental alcoholic liver injury: Relationship to cytochrome P-450 2E1 induction and conjugated diene formation", Alcohol Clin Exp Res, 17, pp.598~603 (1993)
- Németh K, "Effect of supplementation with methionine and different fat sources on the glutathione redox system of growing chickens", Acta Vet Hung, 52, pp.369~378 (2004)
- Porubsky PR, "Structures of human cytochrome P-450 2E1. Insights into the binding of inhibitors and both small molecular weight and fatty acid substrates", J Biol Chem, 283, pp.33698~33707 (2008)
- Reeves PG, "AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition Ad Hoc writing committee on the reformulation of the AIN-76A Rodent diet", J Nutr, 123, pp.1939~1951 (1993)
- Ronis MJ, "Dietary saturated fat reduces alcoholic hepatotoxicity in rats by altering fatty acid metabolism and membrane composition", J Nutr, 134, pp.904~912 (2004)
- Roskams T, "Oxidative stress and oval cell accumulation in mice and humans with alcoholic and nonalcoholic fatty liver disease", Am J Pathol, 163, pp.1301~1311 (2003)
- Sedlak J, "Estimation of total, protein-bounded and nonprotein sulfhydryl groups in tissues with Ellman’ Reagent", Anal Biochem, 25, pp.192~205 (1968)
- Slim RM, "Susceptibility to hepatic oxidative stress in rabbits fed different animal and plant fats", J Am Coll Nutr, 15, pp.289~294 (1996)
- Speck RF, "Fish oil protects mice against acetaminophen hepatotoxicity in vivo", Hepatology, 13, pp.557~561 (1991)
- Tamura H, "Gas chromatographic analysis of malonaldehyde and 4-hydroxy-2-(E)-nonenal produced from arachidonic and linoleic acid in a lipid peroxidation model system", Lipids, 26, pp.170~173 (1991)