Lipid-lowering and hepatoprotective effects of Vitis vinifera dried seeds on paracetamol-induced hepatotoxicity in rats
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BACKGROUND/OBJECTIVES: Red grape seeds as functional food are a good source of important bioactive components suchas phenolics and antioxidants, which decrease oxidative stress that contributes to the pathogenesis of hepatotoxicity. Thecurrent study was conducted in order to evaluate the protective effect of red grape dried seeds (RGDS) on antioxidant properties,lipid metabolism, and liver and kidney functions of rats with paracetamol (750 mg/kg) induced hepatotoxicity. MATERIALS/METHODS: RGDS was added to the basal diet at 5, 10, and 20%. Thirty five adult male rats were assigned tofive groups (n = 7) for a six-week feeding period; group (1) normal control, group (2) induced control, groups (3, 4, and 5)fed a diet with RGPS at different levels, 5, 10, and 20%, respectively. At the end of the feeding period, animals’ blood andtissues were collected for estimation of serum lipid profile, serum liver, and kidney biomarkers. The protection was measuredby detecting lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD), Catalase (CAT) (in liver tissues), and liverhistological examination. RESULTS: The results showed a significant (P < 0.05) decrease in levels of serum cholesterol, triglycerides, low density lipoprotein(LDL-C), and very low density lipoprotein (VLDL-C), with a significant increase in level of high density lipoprotein (HDL-C) forRGDS groups compared to induced control. Rats administered a diet containing RGDS levels produced significant (P < 0.05)hepatoprotection by decreasing the activities of liver enzymes, kidney parameters, and lipid peroxidation, while levels of GSH,SOD, and CAT were increased significantly to near the normal levels. CONCLUSION: The RGDS 20% group was more effective than others against hepatotoxicity of paracetamol, which may beattributed to RGDS total phenols and antioxidant contents, which were 1.438 mg and 1.231 mg, respectively.
Abstract
BACKGROUND/OBJECTIVES: Red grape seeds as functional food are a good source of important bioactive components suchas phenolics and antioxidants, which decrease oxidative stress that contributes to the pathogenesis of hepatotoxicity. Thecurrent study was conducted in order to evaluate the protective effect of red grape dried seeds (RGDS) on antioxidant properties,lipid metabolism, and liver and kidney functions of rats with paracetamol (750 mg/kg) induced hepatotoxicity. MATERIALS/METHODS: RGDS was added to the basal diet at 5, 10, and 20%. Thirty five adult male rats were assigned tofive groups (n = 7) for a six-week feeding period; group (1) normal control, group (2) induced control, groups (3, 4, and 5)fed a diet with RGPS at different levels, 5, 10, and 20%, respectively. At the end of the feeding period, animals’ blood andtissues were collected for estimation of serum lipid profile, serum liver, and kidney biomarkers. The protection was measuredby detecting lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD), Catalase (CAT) (in liver tissues), and liverhistological examination. RESULTS: The results showed a significant (P < 0.05) decrease in levels of serum cholesterol, triglycerides, low density lipoprotein(LDL-C), and very low density lipoprotein (VLDL-C), with a significant increase in level of high density lipoprotein (HDL-C) forRGDS groups compared to induced control. Rats administered a diet containing RGDS levels produced significant (P < 0.05)hepatoprotection by decreasing the activities of liver enzymes, kidney parameters, and lipid peroxidation, while levels of GSH,SOD, and CAT were increased significantly to near the normal levels. CONCLUSION: The RGDS 20% group was more effective than others against hepatotoxicity of paracetamol, which may beattributed to RGDS total phenols and antioxidant contents, which were 1.438 mg and 1.231 mg, respectively.
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