Comparative Morphological Study on Parotid and Submandibular Salivary Glands in Ovariectomized Rats
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Estrogen deficiency affects the structure and function of the salivary glands in women, leading to a decrease in salivary secretion and a change in the composition of saliva. Previous studies on changes in the salivary glands that cause estrogen deficiency have reported only partial results for the parotid and submandibular glands, and there are few comparative morphological studies of histological changes between the parotid and submandibular glands in ovariectomized rats (OVX) leading to estrogen deficiency. This study aimed to analyze the histopathological and histochemical changes in the parotid and submandibular salivary glands causing estrogen deficiency by using OVX, and to discuss the mechanism on these changes.Methods: The parotid and submandibular glands from sacrificed control and OVX groups were fixed with cold 4% paraformaldehyde in phosphate buffer (pH 7.2). The tissues were dehydrated using a series of graded ethyl alcohol and embedded in paraffin. For histopathological analysis, sections cut to a thickness of 6 to 7 µm were stained with hematoxylin and eosin (H&E). For histochemical analysis, Periodic acid-Schiff (PAS), Alcian blue (AB, pH 2.5), and PAS+AB (pH 2.5 and pH 1) staining was performed.Results: Histopathological analysis of OVX tissue showed that the parotid and submandibular salivary glands were broadly and clearly separated and divided into lobes. In OVX, acinar and ductal cells with condensed polymorphic or pyknotic nucleus, which are presumed to be characteristic of apoptotic cells, and degenerated cells with lipid deposition in cytoplasmic granules and ruptured membranes were increased. Histochemical analysis of OVX, confirmed an increase in the number and acidification of acinar secretory granules.Conclusion: Histopathological and histochemical changes and the effects of estrogen deficiency are more evident in the submandibular salivary gland than in the parotid gland.
Abstract
Background: Estrogen deficiency affects the structure and function of the salivary glands in women, leading to a decrease in salivary secretion and a change in the composition of saliva. Previous studies on changes in the salivary glands that cause estrogen deficiency have reported only partial results for the parotid and submandibular glands, and there are few comparative morphological studies of histological changes between the parotid and submandibular glands in ovariectomized rats (OVX) leading to estrogen deficiency. This study aimed to analyze the histopathological and histochemical changes in the parotid and submandibular salivary glands causing estrogen deficiency by using OVX, and to discuss the mechanism on these changes.Methods: The parotid and submandibular glands from sacrificed control and OVX groups were fixed with cold 4% paraformaldehyde in phosphate buffer (pH 7.2). The tissues were dehydrated using a series of graded ethyl alcohol and embedded in paraffin. For histopathological analysis, sections cut to a thickness of 6 to 7 µm were stained with hematoxylin and eosin (H&E). For histochemical analysis, Periodic acid-Schiff (PAS), Alcian blue (AB, pH 2.5), and PAS+AB (pH 2.5 and pH 1) staining was performed.Results: Histopathological analysis of OVX tissue showed that the parotid and submandibular salivary glands were broadly and clearly separated and divided into lobes. In OVX, acinar and ductal cells with condensed polymorphic or pyknotic nucleus, which are presumed to be characteristic of apoptotic cells, and degenerated cells with lipid deposition in cytoplasmic granules and ruptured membranes were increased. Histochemical analysis of OVX, confirmed an increase in the number and acidification of acinar secretory granules.Conclusion: Histopathological and histochemical changes and the effects of estrogen deficiency are more evident in the submandibular salivary gland than in the parotid gland.
참고문헌 (26)
- Toan NK, "Aging-related metabolic dysfunction in the salivary gland : a review of the literature", Int J Mol Sci, 22, pp.5835~ (2021)[DOI]
- Parlak SN, "Effects of menopause and diabetes on the rat parotid glands : a histopathological and stereological study", Int J Med Sci Public Health, 3, pp.749~755 (2014)[DOI]
- Välimaa H, "Estrogen receptorbeta is the predominant estrogen receptor subtype in human oral epithelium and salivary glands", J Endocrinol, 180, pp.55~62 (2004)[DOI]
- Kullander S, "Studies on saliva in menstruating, pregnant and post-menopausal women", Acta Endocrinol (Copenh), 48, pp.329~336 (1965)[DOI]
- Choe JK, "Progesterone and estradiol in the saliva and plasma during the menstrual cycle", Am J Obstet Gynecol, 147, pp.557~562 (1983)[DOI]
- Laine M, "Pregnancy-related changes in human whole saliva", Arch Oral Biol, 33, pp.913~917 (1988)[DOI]
- Salvolini E, "Biochemical modifications of human whole saliva induced by pregnancy", Br J Obstet Gynaecol, 105, pp.656~660 (1998)[DOI]
- Streckfus CF, "Effects of estrogen status and aging on salivary flow rates in healthy Caucasian women", Gerontology, 44, pp.32~39 (1998)[DOI]
- Yalçin F, "The effect of menopause, hormone replacement therapy(HRT), alendronate(ALN), and calcium supplements on saliva", J Contemp Dent Pract, 6, pp.10~17 (2005)[DOI]
- Laine M, "Effect of hormone replacement therapy on salivary flow rate, buffer effect and pH on perimenopausal and postmenopausal women", Arch Oral Biol, 41, pp.91~96 (1996)[DOI]
- Laine M, "Effects of low-dose oral contraceptives on female whole saliva", Arch Oral Biol, 36, pp.549~552 (1991)[DOI]
- Meurman JH, "The menopause and oral health", Maturitas, 63, pp.56~62 (2009)[DOI]
- Kim JH, "Effects of oligonol on the submandibular gland in ovariectomized rats", Biomed Pharmacother, 141, pp.111897~ (2021)[DOI]
- 정순정, "Morphological Study on the Correlation of Prenatal and Postnatal Development between Mouse Parotid Salivary Gland and Tooth", Applied Microscopy, 47(4), pp.242~250 (2017), 한국현미경학회
- Jeong SJ, "Ultrastructure of the submandibular gland in the big white-toothed shrew, Crocidura lasiura", Appl Microsc, 35, pp.57~64 (2005)
- Laine M, "Salivary buffer effect in relation to late pregnancy and postpartum", Acta Odontol Scand, 58, pp.8~10 (2000)[DOI]
- Uyanıkgil Y, "The effects of ovariectomy on the submandibular gland in young female adult rats", Ege J Med, 50, pp.7~11 (2011)
- Kwon HK, "The mechanism of submandibular gland dysfunction after menopause may be associated with the ferroptosis", Aging (Albany NY), 12, pp.21376~21390 (2020)[DOI]
- Da Y, "A comparison of the effects of estrogen and Cimicifuga racemosa on the lacrimal gland and submandibular gland in ovariectomized rats", PLoS One, 10, pp.e0121470~ (2015)[DOI]
- Gouda ZA, "Mechanistic effect of human umbilical cord blood derived mesenchymal stem cells on the submandibular salivary gland in ovariectomized rats", Biochem Cell Biol, 96, pp.57~67 (2018)[DOI]
- Kusunoki T, "The role of estrogen and Cu, Zn-SOD on histological changes after menopause in female rat parotid", Auris Nasus Larynx, 33, pp.47~51 (2006)[DOI]
- Seko K, "Effects of ovariectomy and estrogen replacement on rat oral mucosa", Maturitas, 50, pp.44~51 (2005)[DOI]
- Eltokhey HM, "Tibolone improves the degenerative changes of tongue mucosa in ovariectomized female rats", J Cytol Histol, 3, pp.1000130~ (2012)[DOI]
- Ishimaru N, "Development of autoimmune exocrinopathy resembling Sjögren's syndrome in estrogen-deficient mice of healthy background", Am J Pathol, 163, pp.1481~1490 (2003)[DOI]
- Green S, "Human oestrogen receptor cDNA : sequence, expression and homology to v-erb-A", Nature, 320, pp.134~139 (1986)[DOI]
- Kuiper GG, "Cloning of a novel receptor expressed in rat prostate and ovary", Proc Natl Acad Sci U S A, 93, pp.5925~5930 (1996)[DOI]