본문 바로가기
KERD
논문KCI 등재

감잎의 광노화 피부 개선에 미치는 효과(2)

Effects of Persimmon leaf on the Photoaging Skin Improvement(2)

김남석|권진|최동성|오미진|마상용|김명순|신현종|이창현|유승정|오찬호
한의병리학회 (The Society of Pathology in Korean Medicine)|2014.02|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제28권 제1호|pp. 35-44

2

조회

0

다운로드

2

인용

0

참고문헌

키워드

ROSRT-PCRMMPAnti-photoagingpersimmon leafhairless mice(SKH-1)gelatin zymography
주제분류한의학

초록

This study was performed to investigate the anti-photoaging effects of Persimmon leaf tea(PLT) in hairless mice(SKH-1) exposed to UVB radiation. The animals were divided into non-treated group (normal, N) and UV-radiated groups. UV-radiated groups were divided into only UV-radiated group(control, C) and UV-radiated and PLT treated experimental groups[first extraction treated group(PLT-I), second extraction treated groupe(PLT-II), and third extraction treated group(PLT-III)]. Three PLT treated experimental groups of mice were treated with both oral administration(300mg/Kg B.W./day) and topical application (100 ul of 2% conc./mouse/day) for 4 weeks. Anti-photoaging effects of Persimmon leaf were evaluated by MTT assay, anti oxidative reaction, MMP immunohistochemistry, gelatin zymography assay and RT-PCR observations. Treatment with Persimmon leaf tea(PLT)-I, and -III groups decreased immunohistochemical density of matrix metalloproteinases(MMP)-3 and -9 related to degradation of extracellular matrix in skin. Especially, immunohistochemical density of MMP-2 decreased in PLT-I, -II and –III groups in skin. On the effects of antioxidant function on the treatment with Persimmon leaf tea(PLT), treatment of HaCaT cells with extracts of PLT-I and PLT-II had also significantly reduced intracellular ROS produced by UVB irradiation in a dose dependent manner(PLT-I, p<0.05, p<0.01, p<0.001; PLT-II, p<0.01, p<0.001). Gelatin zymography assay revealed that PLT-II and PLT-III (200 ug/ml) had inhibitory effect on MMP-9 expression in UVB-radiated HaCaT cells. Western blot analysis revealed that PLT-1, -II and -III groups down-regulates the expression of inflammatory associated genes(IL-1β) and PLT-1 and -II groups down-regulates the expression of TNF-α in a dose dependent manner. Our study suggests that Persimmon leaf tea(PLT) extracts participates in inhibitory effects on the morphological and molecular experiments related to photoaging skin on UVB irradiated hairless mice.

Abstract

This study was performed to investigate the anti-photoaging effects of Persimmon leaf tea(PLT) in hairless mice(SKH-1) exposed to UVB radiation. The animals were divided into non-treated group (normal, N) and UV-radiated groups. UV-radiated groups were divided into only UV-radiated group(control, C) and UV-radiated and PLT treated experimental groups[first extraction treated group(PLT-I), second extraction treated groupe(PLT-II), and third extraction treated group(PLT-III)]. Three PLT treated experimental groups of mice were treated with both oral administration(300mg/Kg B.W./day) and topical application (100 ul of 2% conc./mouse/day) for 4 weeks. Anti-photoaging effects of Persimmon leaf were evaluated by MTT assay, anti oxidative reaction, MMP immunohistochemistry, gelatin zymography assay and RT-PCR observations. Treatment with Persimmon leaf tea(PLT)-I, and -III groups decreased immunohistochemical density of matrix metalloproteinases(MMP)-3 and -9 related to degradation of extracellular matrix in skin. Especially, immunohistochemical density of MMP-2 decreased in PLT-I, -II and –III groups in skin. On the effects of antioxidant function on the treatment with Persimmon leaf tea(PLT), treatment of HaCaT cells with extracts of PLT-I and PLT-II had also significantly reduced intracellular ROS produced by UVB irradiation in a dose dependent manner(PLT-I, p<0.05, p<0.01, p<0.001; PLT-II, p<0.01, p<0.001). Gelatin zymography assay revealed that PLT-II and PLT-III (200 ug/ml) had inhibitory effect on MMP-9 expression in UVB-radiated HaCaT cells. Western blot analysis revealed that PLT-1, -II and -III groups down-regulates the expression of inflammatory associated genes(IL-1β) and PLT-1 and -II groups down-regulates the expression of TNF-α in a dose dependent manner. Our study suggests that Persimmon leaf tea(PLT) extracts participates in inhibitory effects on the morphological and molecular experiments related to photoaging skin on UVB irradiated hairless mice.

참고문헌 (56)

  1. Meinhard, W., "Solar UV irradiation and dermal photoaging", J Photochem and Photobiology, 63, pp.41~51 (2001)
  2. Gilchrest, B.A., "Skin aging and photoaging: an overview", J Am Acad Dermatol, 989(21), pp.610~613 (1989)
  3. Griffiths, C.E.M., "The clinical identification and quantification of photodamage", Br J Dermatol, 127(41), pp.37~42 (1992)
  4. Lavker, R.M., "Structural alterations in exposed and unexposed aged skin", J Invest Dermatol, 73, pp.59~66 (1979)
  5. Kligman, A.M., "Topical tretinoin for photodamaged skin", J Am Acad Dermatol, 15, pp.836~859 (1986)
  6. Berneburg, M., "Photoaging of human skin", Photodermatol Photoimmunol Photomed, 16, pp.239~244 (2000)
  7. Pillai, S., "Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review", Int J Cosmet Sci, 27, pp.17~24 (2005)
  8. Kehrer, J.P., "Free radicals as mediators of tissue injury and disease", CRC Crit Rev Toxicol, 23, pp.21~28 (1993)
  9. Aruoma, O.I., "Nutrition and health aspects of free radicals and antioxidant", Food Chem Toxicol, 62, pp.671~683 (1994)
  10. 박창민, "3-O-cetyl-L-ascorbic acid의 주름 개선 효과", 대한화장품학회지, 34(4), pp.303~309 (2008), 대한화장품학회
  11. Wyss, R., "Chromatographic and electrophoretic analysis of biomedically important retinoids", J Chromatogr B, Biomed Sci Appl, 671, pp.381~425 (1995)
  12. Weiss, J.S., "Topical tretinoin in the treatment of aging skin", J Am Acad Dermatol, 19, pp.169~175 (1988)
  13. Gary, J.F., "Mechanisms of photoaging and chronological skin aging", Arch Dermatol, 138, pp.1462~1470 (2002)
  14. Kim, Y.G., "Effects of ginseng saponins isolated from red ginseng on ultraviolet B-induced skin aging in hairless mice", Eur J Pharm, 602, pp.148~156 (2009)
  15. 전데레사, "홍차 열수추출물의 피부노화 개선 효과", 대한미용학회지, 7(4), pp.441~447 (2011), 대한미용학회
  16. Sang-Jin Lee, "Inhibitory Effects of Ethanol Extracts from Nuruk on Oxidative Stress, Melanogenesis, and Photo-Aging", Mycobiology, 40(2), pp.117~123 (2012), 한국균학회[DOI]
  17. 손종연, "한국 전통차의 생리활성 및 항산화작용", 한국식품조리과학회지, 27(5), pp.567~575 (2011), 한국식품조리과학회
  18. 오현명, "Effects of Dried leaf powders, water and ethanol extracts of persimmon and green tea leaves on lipid metabolism and antioxidative capacity in 12-month-old rats", 한국식품영양과학회지, 34(3), pp.285~298 (2001)
  19. Joung, S.Y., "The chemical composition of persimmon(Diospyros kaki, thumb) leaf tea", J Korean Soc Food Nutr, 24(5), pp.720~726 (1995)
  20. Uchida, S., "Condensed tannins scavenge active oxygen free radicals", Med Sci Res, 15(5), pp.831~834 (1987)
  21. Kameda, K., "Inhibitory effects of various flavonoids isolate from leaf of persimmon on angiotensin-converting enzyme activity", J Nat Products, 50(4), pp.680~686 (1987)
  22. 여생규, "Antioxidative effect of tea extracts from green tea, oolong tea and black tea", 한국식품영양과학회지, 24(2), pp.299~304 (1995)
  23. Hsu, S.M., "Use of avidin-biotin-peroxidase complex(ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody(PAP) procedures", J Histochem Cytochem, 29, pp.577~580 (1981)
  24. Mosmann, Tim., "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J Immunol Methods, 65(1), pp.55~63 (1983)
  25. Ha, K.T., "Inhibitory effect of Daesungki-Tang on the invasiveness potential of hepatocellular carcinoma through inhibition of matrix metalloproteinase-2 and -9 activities", Toxicol Appl Pharmacol, 200(1), pp.1~6 (2004)
  26. Placzec, M., "Tobacco smoke is phototoxic", Br J Dermatol, 150, pp.991~993 (2004)
  27. Fisher, G.J., "Mechanisms of photoaging and chronological skin aging", Arch Dermatol, 138, pp.1462~1470 (2002)
  28. Landau, M., "Exogenous factors in skin aging", Curr Probl Dermatol, 35, pp.1~13 (2007)
  29. Cooper, K.D., "UV exposure reduces immunization rates and promotes tolerance to epicutaneous antigens in humans: relationship to dose, CD1a-DR epidermal macrophage induction, and Langerhans cell depletion", Proc Natl Acad Sci USA, 89, pp.8497~8501 (1992)
  30. Fisher, G.J., "Pathophysiology of premature aging induced by ultraviolet light", N Engl J Med, 337, pp.1419~1428 (1997)
  31. Sander, C.S., "Photoaging is associated with protein oxidation in human skin in vivo", J Invest Dermatol, 118(4), pp.618~625 (2002)
  32. Chung, J. H., "Modulation of skin collagen metabolism in aged and photoaged human skin in vivo", J Invest Dermatol, 117, pp.1218~1224 (2001)
  33. Gilchrest, B.A., "A review of skin aging and its medical therapy", Br J Dermatol, 135, pp.867~875 (1996)
  34. Fisher, G.J., "Molecular basis of sun-induced premature skin aging and retinoid antagonism", Nature, 379(6563), pp.335~339 (1996)
  35. Kiss, I., "The effect of high and low ultraviolet-B dose exposure on the degree of hairless mouse skin wrinkling", Photochem Photobiol, 53, pp.109~112 (1991)
  36. Seo, J. Y., "Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skin in vivo", J Invest Dermatol, 116, pp.915~919 (2001)
  37. 안덕균, "한국본초도감", pp.460~ (1999), 교학사
  38. 이경순, "완역 중약대사전", pp.2614~ (1997), 정담
  39. Moon, S.H., "Antitumor effect of persimmon leaves in vivo using sarcoma-180 cells", J Korean Soc Food Sci Nutr, 25, pp.865~870 (1996)
  40. Jie Ma, "Protective Effects of Persimmon Leaf and Fruit Extracts against Acute Ethanol-Induced Hepatotoxicity", Preventive Nutrition and Food Science, 12(4), pp.202~208 (2007), 한국식품영양과학회
  41. Kim, E.S., "Effect of dried leaf powders and ethanol extracts of persimmon, green tea and pine needle on lipid metabolism and antioxidative capacity in rats", Korean J Nutr, 32, pp.337~352 (1999)
  42. Moon, S. H., "Antimutagenic effects of boiled water extract and tannin from persimmon leaves", J Korean Soc Food Nutr, 24, pp.880~886 (1995)
  43. 박윤주, "Changes of vitamin C and superoxide dismutase(SOD)-like activity of Persimmon leaf tea by processing method and extraction condition", 한국식품과학회지, 27(3), pp.281~285 (1995)
  44. 문숙희, "Antioxidative effect of Persimmon leaves", 한국식품영양학회지, 13(1), pp.53~55 (2000)
  45. 송현순, "Antimutagenic effects of persimmon leaf tea extract (PLTE) in mice using micronucleus induction (MN) Test", 한국식품영양과학회지, 29(5), pp.881~887 (2000)
  46. 김옥경, "Effects of extracts of Diospyrus kaki folium against hepatotoxicity in carbon tetrachloride intoxicated rats", 한국식품영양과학회지, 30(1), pp.97~101 (2001)
  47. 박무희, "Effect of polyphenol compounds from Persimmon leaves (Diospyros kaki folium) on allergic contact dermatitis", 한국식품영양과학회지, 29(1), pp.111~115 (2000)
  48. An, B.J., "Inhibition of enzyme activities and the antiwrinkle effect of polyphenol isolated from the persimmon leaf (Diospyros kaki folium) on human skin", Dermatol Surg, 31(7), pp.848~854 (2005)
  49. Shindo, Y., "Antioxidants defense mechanisms in murine epidermis and dermis and their responses to ultraviolet light", J Invest Dermatol, 100, pp.260~265 (1993)
  50. Masak, H., "Detection of hydroxygen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation", Biochem Biophys Res Commun, 206, pp.474~479 (1995)
  51. Pinkus, R., "Role of oxidants and antioxidants in the induction of AP-1, NF-κB, and glutathione S-transferase gene expression", Biol Chem, 271(23), pp.13422~13429 (1996)
  52. Guyton, K.Z., "Induction of the mammalian stress response gene GADD153 by oxidative stress: role of AP-1 element", Biochem J, 314(2), pp.547~554 (1996)
  53. Schreiber, M., "Fos is an essential component of the mammalian UV response", EMBO J, 14, pp.5338~5349 (1995)
  54. Birkedal-Hansen, H., "Proteolytic remodeling of extracellular matrix", Curr Opin Cell Biol, 7, pp.728~735 (1995)
  55. Reynolds, JJ., "Collagenases and tissue inhibitors of metalloproteinases: a functional balance in tissue degradation", Oral Dis, 2, pp.70~76 (1996)
  56. Inomata, S., "Possible involvement of gelatinases in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse", J Invest Dermatol, 120, pp.128~134 (2003)

관련 논문AI추천

감잎의 광노화 피부 개선에 미치는 효과(1)권진, 김남석, 김명순, 마상용, 신현종, 오미진, 오찬호, 유승정, 이창현, 최동성한의병리학회 · 동의생리병리학회지2013 · 제27권 제6호UVB로 조사된 Hairless 마우스에서 홍차 열수추출물의 피부주름 개선 효과김영철, 장병수, 최소영, 전데레사대한미용학회 · 대한미용학회지2011 · 제7권 제3호Protective effect of the standardized green tea seed extract on UVB-induced skin photoaging in hairless mice강남길, 김옥경, 이민재, 이정민, 임재연, 황재관한국영양학회 · Nutrition Research and Practice2014 · 제8권 제4호백차 열수추출물의 자외선 조사로 형성된 피부주름 개선 효과김영철, 이경옥, 장병수대한미용학회 · 대한미용학회지2011 · 제7권 제4호발효홍삼 추출물의 광노화 피부 보호 효과권진, 김종석, 김호일, 오미진, 이성철, 이창현한의병리학회 · 동의생리병리학회지2015 · 제29권 제1호자외선 조사에 의해 노화된 인간각질형성세포에서 감잎 추출물의 항노화 효능박선영, 심중현한국피부과학연구원 · 아시안뷰티화장품학술지2017 · 제15권 제2호

이 간행물의 다른 논문 (18건)

당귀사역가오수유생강탕의 문헌적 고찰홍성인, 송윤경, 고호연, 전찬용, 정기용, 박종형, 이용현, 차윤엽, 김태훈, 박정수, 정종진, 선승호, 임은미, 고성규, 최유경pp. 1-8대만 중·서의 의료이원화 현황에 관한 연구- 면허제도 및 교육제도 확립을 중심으로 -권영규, 김동수, 권수현, 이현지, 최병희pp. 9-15미세침요법의 모발성장효과이지연, 정한솔, 신현종, 하기태, 이창현, 서형식pp. 16-21유방암세포에서 괴화 에탄올 추출물의 NF-kB 의존적인 MMP-9 발현의 조절 규명을 위한 연구박연주, 김정미, 김하림, 김미성, 황진기, 이영래, 류도곤, 권강범, 유용욱, 김성철pp. 22-28대추 물 추출물이 RANKL에 의해 유도되는 파골세포 분화에 미치는 영향곽성철, 백종민, 오재민, 김주영, 이명수, 천윤희, 김정중, 이창훈, 전병훈, 최민규, 윤강휴pp. 29-34
원문 보기