Determination and Validation of Method Based on Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry for Methimazole in Livestock and Fishery Product
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초록
Carbimazole is used for the treatment of hyperthyroidism. Methimazole, which is produced after the rapid metabolization of carbimazole in the body, can adversely affect endocrine hormones and cause gastrointestinal problems. Methimazole is prescribed as a veterinary drug to treat thyroid disease in cats. However, there is suspicion that it is being used for rapid fattening of non-thyroidal livestock, such as cows and pigs, posing serious health risks to consumers. Currently, there are no analytical methods for the detection of methimazole in livestock and fishery products in Korea. Thus, in this study, we developed a method to efficiently analyze methimazole in livestock and fishery products through liquid chromatography with tandem mass spectrometry. Methimazole was extracted with 80% acetonitrile and purified using C18 powder, followed by fat removal using acetonitrile-saturated hexane. The coefficient of linearity, average recovery, and coefficient of variation of the analytical method were verified according to the CODEX guidelines. The developed method was employed to monitor methimazole residue levels in livestock and fishery products, but no residues were detected. The proposed method can serve as a foundation for improving the safety management of domestic- and imported-livestock and fishery products.
Abstract
Carbimazole is used for the treatment of hyperthyroidism. Methimazole, which is produced after the rapid metabolization of carbimazole in the body, can adversely affect endocrine hormones and cause gastrointestinal problems. Methimazole is prescribed as a veterinary drug to treat thyroid disease in cats. However, there is suspicion that it is being used for rapid fattening of non-thyroidal livestock, such as cows and pigs, posing serious health risks to consumers. Currently, there are no analytical methods for the detection of methimazole in livestock and fishery products in Korea. Thus, in this study, we developed a method to efficiently analyze methimazole in livestock and fishery products through liquid chromatography with tandem mass spectrometry. Methimazole was extracted with 80% acetonitrile and purified using C18 powder, followed by fat removal using acetonitrile-saturated hexane. The coefficient of linearity, average recovery, and coefficient of variation of the analytical method were verified according to the CODEX guidelines. The developed method was employed to monitor methimazole residue levels in livestock and fishery products, but no residues were detected. The proposed method can serve as a foundation for improving the safety management of domestic- and imported-livestock and fishery products.
참고문헌 (24)
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