LC-MS/MS를 이용한 축·수산물 중 β-agonist계 시험법 개발 및 검증
Development and Validation of an Analytical Method for β-Agonists in Livestock and Fishery Products Using LC-MS/MS
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
BACKGROUND: The β-agonists known as phenyl etha-nolamine derivatives have a conjugated aromatic ring with amino group. They are used as tocolytic agents and bronchodilator to human and animal generally, and some of them are used as growth promoters to livestock. METHODS AND RESULTS: β-agonists in samples were extracted by 0.4 N perchloric acid and ethyl acetate. The target compounds were analyzed by liquid chromatog-raphy-electrospray tandem mass spectrometry (LC-ESI- MS/MS). Validation of method was performed according to CODEX guidelines (CAC/GL-71). The matrix match-ed calibration gave correlation coefficients>0.98, and the obtained recoveries were in the range of 62.0-109.8%, with relative standard deviation ≤ 20.1%. In addition, a survey was performed to inspect any residual β-agonist from 100 samples of livestock and fishery products and ractopamine was detected in one of the 100 samples. CONCLUSION(S): In this study, we established the ana-lytical method for β-agonists through using the expanded target compounds and samples. And we anticipate that the established method would be used for analysis to determine veterinary drug residues in livestock and fishery products.
Abstract
BACKGROUND: The β-agonists known as phenyl etha-nolamine derivatives have a conjugated aromatic ring with amino group. They are used as tocolytic agents and bronchodilator to human and animal generally, and some of them are used as growth promoters to livestock. METHODS AND RESULTS: β-agonists in samples were extracted by 0.4 N perchloric acid and ethyl acetate. The target compounds were analyzed by liquid chromatog-raphy-electrospray tandem mass spectrometry (LC-ESI- MS/MS). Validation of method was performed according to CODEX guidelines (CAC/GL-71). The matrix match-ed calibration gave correlation coefficients>0.98, and the obtained recoveries were in the range of 62.0-109.8%, with relative standard deviation ≤ 20.1%. In addition, a survey was performed to inspect any residual β-agonist from 100 samples of livestock and fishery products and ractopamine was detected in one of the 100 samples. CONCLUSION(S): In this study, we established the ana-lytical method for β-agonists through using the expanded target compounds and samples. And we anticipate that the established method would be used for analysis to determine veterinary drug residues in livestock and fishery products.
참고문헌 (29)
- OECD-FAO Agricultural outlook 2021-2030, "OECD", 163~177 (2021)
- OECD-FAO Agricultural outlook 2021-2030, "OECD", 190~201 (2021)
- Thomas P. Van Boeckel, "Global trends in antimicrobial use in food animals", Proceedings of the National Academy of Sciences, 112(18), pp.5649~5654 (2015), Proceedings of the National Academy of Sciences[DOI]
- S. A. Tittlemier, "Analysis of veterinary drug residues in fish and shrimp composites collected during the Canadian Total Diet Study, 1993–2004", Food Additives and Contaminants, 24(1), pp.14~20 (2007), Informa UK Limited[DOI]
- Don A Franco, "Antibiotic and Sulfonamide Residues in Meat: Implications for Human Health", Journal of Food Protection, 53(2), pp.178~185 (1990), Elsevier BV[DOI]
- ByungSu Yoo, "β1-Adrenergic receptors stimulate cardiac contractility and CaMKII activation in vivo and enhance cardiac dysfunction following myocardial infarction", American Journal of Physiology-Heart and Circulatory Physiology, 297(4), pp.H1377~H1386 (2009), American Physiological Society[DOI]
- M JOHNSON, "Molecular mechanisms of β2-adrenergic receptor function, response, and regulation", Journal of Allergy and Clinical Immunology, 117(1), pp.18~24 (2006), Elsevier BV[DOI]
- B. B. Lowell, MD, PhD, "BROWN ADIPOSE TISSUE, β3-ADRENERGIC RECEPTORS, AND OBESITY", Annual Review of Medicine, 48(1), pp.307~316 (1997), Annual Reviews[DOI]
- K. L. Rogers, "Effect of Beta Agonists on Protein Turnover in Isolated Chick Skeletal and Atrial Muscle", Experimental Biology and Medicine, 197(4), pp.482~485 (1991), SAGE Publications[DOI]
- H J Mersmann, "Overview of the effects of beta-adrenergic receptor agonists on animal growth including mechanisms of action", Journal of Animal Science, 76(1), pp.160~172 (1998), Oxford University Press (OUP)[DOI]
- D J Smith, "The pharmacokinetics, metabolism, and tissue residues of beta-adrenergic agonists in livestock", Journal of Animal Science, 76(1), pp.173~194 (1998), Oxford University Press (OUP)[DOI]
- Alberto Alemanno, "Testing the Limits of Global Food Governance: The Case of Ractopamine", European Journal of Risk Regulation, 3(3), pp.400~407 (2012), Cambridge University Press (CUP)[DOI]
- Zaitseva NV, "Health risk assessment of exposure to ractopamine through consumption of meat products", International Journal of Advanced Research, 2(9), pp.538~545 (2014)
- Hui-Seung Kang, "Occurrence of veterinary drug residues in farmed fishery products in South Korea", Food Control, 85, pp.57~65 (2018), Elsevier BV[DOI]
- JeongWoo Kang, "Veterinary drug residues in domestic and imported foods of animal origin in the Republic of Korea", Food Additives & Contaminants: Part B, 8(2), pp.106~112 (2015), Informa UK Limited[DOI]
- Kyuchukova R, "Antibiotic residues and human health hazard-review", Bulgarian Journal of Agricultural Science, 26(3), pp.664~668 (2020)
- Gianfranco Brambilla, "Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beef meat in Italy", Toxicology Letters, 114(1-3), pp.47~53 (2000), Elsevier BV[DOI]
- Ole E. Heuer, "Human Health Consequences of Use of Antimicrobial Agents in Aquaculture", Clinical Infectious Diseases, 49(8), pp.1248~1253 (2009), Oxford University Press (OUP)[DOI]
- Evelina Tacconelli, "Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis", The Lancet Infectious Diseases, 18(3), pp.318~327 (2018), Elsevier BV[DOI]
- I. Thung, "The global emergence of Helicobacter pylori antibiotic resistance", Alimentary Pharmacology and Therapeutics, 43(4), pp.514~533 (2015), Wiley[DOI]
- 최기돈, "국내 Helicobacter pylori 제균치료의 최신동향", Korean Journal of Helicobacter Upper Gastrointestinal Research, 15(3), pp.147~154 (2015), 대한상부위장관ㆍ헬리코박터학회[DOI]
- Reuben Chukwuka Okocha, "Food safety impacts of antimicrobial use and their residues in aquaculture", Public Health Reviews, 39(1), pp.1~22 (2018), Springer Science and Business Media LLC[DOI]
- Reuben Chukwuka Okocha, "Food safety impacts of antimicrobial use and their residues in aquaculture", Public Health Reviews, 39(1), pp.1~22 (2018), Springer Science and Business Media LLC[DOI]
- Daniel R. Doerge, "Detection and Confirmation of β-Agonists in Bovine Retina Using LC", APCI-MS, 1996, pp.68(11), 1918~1923[DOI]
- C. Juan, "Development and validation of a liquid chromatography tandem mass spectrometry method for the analysis of β-agonists in animal feed and drinking water", Journal of Chromatography A, 1217(39), pp.6061~6068 (2010), Elsevier BV[DOI]
- Lin Xiong, "Simple and sensitive monitoring of β2-agonist residues in meat by liquid chromatography–tandem mass spectrometry using a QuEChERS with preconcentration as the sample treatment", Meat Science, 105, pp.96~107 (2015), Elsevier BV[DOI]
- Kunping Yan, "Rapid screening of toxic salbutamol, ractopamine, and clenbuterol in pork sample by high-performance liquid chromatography—UV method", Journal of Food and Drug Analysis, 24(2), pp.277~283 (2016), The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA)[DOI]
- Rong Liu, "Simultaneous determination of fourteen β2-agonist enantiomers in food animal muscles by liquid chromatography coupled with tandem mass spectrometry", Journal of Chromatography B, 1193, pp.123169~ (2022), Elsevier BV[DOI]
- Bing Shao, "Multi-residual analysis of 16 β-agonists in pig liver, kidney and muscle by ultra performance liquid chromatography tandem mass spectrometry", Food Chemistry, 114(3), pp.1115~1121 (2009), Elsevier BV[DOI]