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三稜이 子宮頸部癌細胞(HeLa cell)의 Apoptosis에 미치는 影響

Rhizoma Scirpi induced Apoptosis in Human Cervical Carcinoma HeLa Cells

김주연|최창민|공복철|홍기철|유심근
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2005.11|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제18권 제4호|pp. 10-23

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Abstract

Purpose : This study is to examine the ability of Rhizoma Scirpi (RS) to induce HeLa cell viability. Methods : We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below 37℃, 5% CO2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically. Results : 1. RS induces mitochondria membrane potential collapse. 2. P38 MAPK is involved in RS-induced death in HeLa cells. 3. P38 MAPK is involved in RS-induced apoptosis in HeLa cells. 4. P38 MAPK reguates RS-induced caspase-3, -8 and -9 activation in HeLa cells. 5. The inhibition of caspase regulates RS-induced cell death in HeLa cells. 6. RS induces mitochondria membrane potential collapse in HeLa cells. 7. P38 MAPK is involved in the regulation of Bcl-2 and Bax in HeLa cells. 8. RS regulates the expression of Bcl-2 and Bax in HeLa cells. 9. SR induces p38 MAPK activation in HeLa cells. Conclusion : RS induces apoptosis in HeLa cells via p38 MAPK activation.

참고문헌 (37)

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