Correlation Between Bone Marrow Blasts Counts With Flow Cytometry and Morphological Analysis in Myelodysplastic Syndromes
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Dear Editor, Determination of the percentage of blasts in the bone marrow (BM) is one of the critical factors in diagnosing MDS. Flow cytometry (FCM) of BM cells has been introduced as an important co-criterion for diagnosing MDS [1-3]. However, FCM has not been well accepted because of the lack of consensus of the criteria to define a phenotypic myeloblast and the appropriate denominator for calculation. Moreover, BM samples contain variable amounts of peripheral blood mixed with immature cells, thereby complicating interpretation. The present study was designed to choose reagent combinations to identify blasts by FCM in MDS patients, to determine which cell mass as the denominator in the process of counting the percentages of blasts, and to reveal whether the aspirates with high proportions of mature neutrophils should be normalized based on the proportion of dim CD16 maturing myeloid cells.
Abstract
Dear Editor, Determination of the percentage of blasts in the bone marrow (BM) is one of the critical factors in diagnosing MDS. Flow cytometry (FCM) of BM cells has been introduced as an important co-criterion for diagnosing MDS [1-3]. However, FCM has not been well accepted because of the lack of consensus of the criteria to define a phenotypic myeloblast and the appropriate denominator for calculation. Moreover, BM samples contain variable amounts of peripheral blood mixed with immature cells, thereby complicating interpretation. The present study was designed to choose reagent combinations to identify blasts by FCM in MDS patients, to determine which cell mass as the denominator in the process of counting the percentages of blasts, and to reveal whether the aspirates with high proportions of mature neutrophils should be normalized based on the proportion of dim CD16 maturing myeloid cells.
참고문헌 (10)
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