Reliable, Accurate Determination of the Leukocyte Differential of Leukopenic Samples by Using Hematoflow Method
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Background: Hematology analyzers may ineffectively recognize abnormal cells, and manual differential counts may be imprecise for leukopenic samples. We evaluated the efficacy of the Hematoflow method for determining the leukocyte differential in leukopenic samples and compared this method with the manual differential method. Methods: We selected 249 blood samples from 167 patients with leukopenia (WBC counts, 500–2,000/μL) for analysis in this study. The EDTA-anticoagulated blood samples were analyzed using an automatic blood cell counter (DxH800; Beckman Coulter, USA) and flow cytometry (FC 500; Beckman Coulter) by using Cytodiff reagent and analysis software (Beckman Coulter). Hematoflow results were selected or calculated from DxH800 and Cytodiff results. Two trained pathologists performed a manual differential count by counting 50–100 cells. Results: The precision of the Hematoflow method was superior to that of the manual method in counting 5 leukocyte subpopulations, immature granulocytes (IGs), and blasts. Blasts were detected in all 45 cases (100%) by Hematoflow. The correlation of the Cytodiff blast count to the reference count was high (r = 0.8325). For all other cell populations, the correlation of the Hematoflow results with the reference count was stronger than that of the other manual counts with the reference count. Conclusions: The Hematoflow differential counting method is more reproducible and sensitive than manual counting, and is relatively easy to perform. In particular, this method detected leukemic blasts more sensitively than manual differential counts. The Hematoflow method is a very useful supplement to automated cell counting.
Abstract
Background: Hematology analyzers may ineffectively recognize abnormal cells, and manual differential counts may be imprecise for leukopenic samples. We evaluated the efficacy of the Hematoflow method for determining the leukocyte differential in leukopenic samples and compared this method with the manual differential method. Methods: We selected 249 blood samples from 167 patients with leukopenia (WBC counts, 500–2,000/μL) for analysis in this study. The EDTA-anticoagulated blood samples were analyzed using an automatic blood cell counter (DxH800; Beckman Coulter, USA) and flow cytometry (FC 500; Beckman Coulter) by using Cytodiff reagent and analysis software (Beckman Coulter). Hematoflow results were selected or calculated from DxH800 and Cytodiff results. Two trained pathologists performed a manual differential count by counting 50–100 cells. Results: The precision of the Hematoflow method was superior to that of the manual method in counting 5 leukocyte subpopulations, immature granulocytes (IGs), and blasts. Blasts were detected in all 45 cases (100%) by Hematoflow. The correlation of the Cytodiff blast count to the reference count was high (r = 0.8325). For all other cell populations, the correlation of the Hematoflow results with the reference count was stronger than that of the other manual counts with the reference count. Conclusions: The Hematoflow differential counting method is more reproducible and sensitive than manual counting, and is relatively easy to perform. In particular, this method detected leukemic blasts more sensitively than manual differential counts. The Hematoflow method is a very useful supplement to automated cell counting.
참고문헌 (19)
- Guerti K, "Performance evaluation of the PENTRA 60C+ automated hematology analyzer and comparison with the ADVIA 2120", Int J Lab Hematol, 31, pp.132~141 (2009)
- Novis DA, "Laboratory productivity and the rate of manual peripheral blood smear review: a college of American pathologists Q-probes study of 95, 141 complete blood count determinations performed in 263 institutions", Arch Pathol Lab Med, 130, pp.596~601 (2006)
- Barnes PW, "The international consensus group for hematology review: suggested criteria for action following automated CBC and WBC differential analysis", Lab Hematol, 11, pp.83~90 (2005)
- Fuentes-Arderiu X, "Between-examiner reproducibility in manual differential leukocyte counting", Accred Qual Assur, 12, pp.653~645 (2007)
- Aapro M, "Prophylaxis of chemotherapy-induced febrile neutropenia with granulocyte colony-stimulating factors: where are we now", Support Care Cancer, 18, pp.529~541 (2010)
- Mac Manus M, "Radiotherapy-associated neutropenia and thrombocytopenia: analysis of risk factors and development of a predictive model", Blood, 89, pp.2303~2310 (1997)
- Pierre RV, "The demise of the eyecount leukocyte differential", Clin Lab Med, 22, pp.279~297 (2002)
- Cherian S, "Evaluation of an 8-color flow cytometric reference method for white blood cell differential enumeration", Cytometry B Clin Cytom, 78, pp.319~328 (2010)
- Björnsson S, "Total nucleated cell differential for blood and bone marrow using a single tube in a five-color flow cytometer", Cytometry B Clin Cytom, 74, pp.91~103 (2008)
- Roussel M, "Refining the white blood cell differential: the first flow cytometry routine application", Cytometry A, 77, pp.552~563 (2010)
- Faucher JL, "6 Markers", 5 Colors Extended White Blood Cell Differential by Flow Cytometry, 2007, pp.71, 934~944
- Shafer JA, "Blood and marrow morphology in acute leukemia patients receiving chemotherapy: a photo-essay", Am J Med Technol, 49, pp.77~90 (1983)
- Jean A, "The new haematology analyzer DxH 800: an evaluation of the analytical performances and leucocyte flags, comparison with the LH 755", Int J Lab Hematol (2010)
- Koenn ME, "Comparison of four automated hematology cell analyzers", Clin Lab Sci, 14, pp.238~242 (2001)
- Campana D, "Detection of minimal residual disease in acute leukemia by flow cytometry", Cytometry, 38, pp.139~152 (1999)
- LeBien TW, "B lymphocytes: how they develop and function", Blood, 112, pp.1570~1580 (2008)
- Klebanoff CA, "CD8+ T-cell memory in tumor immunology and immunotherapy", Immunol Rev, 211, pp.214~224 (2006)
- Thalhammer-Scherrer R, "Role of immunological lymphocyte subset typing as a screening method for lymphoid malignancies in daily routine practice", Cytometry, 42, pp.5~10 (2000)
- Richards SJ, "The role of flow cytometry in the diagnosis of paroxysmal nocturnal hemoglobinuria in the clinical laboratory", Clin Lab Med, 27, pp.577~590 (2007)