Single-dose Intramuscular Injection Toxicology of Danggui Pharmacopuncture (DGP) in Sprague-Dawley Rats
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objectives: The purpose of the study is to assess both the approximate lethal dose and the single dose intramuscular injection toxicity of Danggui (Angelica gigantis radix) pharmacopuncture (DGP) in Sprague-Dawley (SD) rats. Methods: The experiments were conducted at the good laboratory practice (GLP) laboratory, Biotoxtech Co., which is a laboratory approved by the ministry of food and drug safety (MFDS). The study was performed according to the GLP regulation and the toxicity test guidelines of the MFDS (2009) after approval of the institutional animal care and use committee of Biotoxtech. Single doses of DGP were injected intramuscularly into the rats in three test groups of 6 week old SD rats (5 male and 5 female rats per groups) in the amounts of 0.1, 0.5, and 1.0 mL/animal for groups 2, 3, and 4, respectively, and normal saline solution in the amount of 1.0 mL/animal was injected intramuscularly into the rats (5 male and 5 female rats) in the control group. Observations of the general symptoms and weight measurements were performed during the 14 day observation period after the injection. Hematologic and serum biochemical examination, necropsy, and a local tolerance test at the injection site were done after the observation period. Results: No death was observed in three test groups (0.1, 0.5 and 1.0 mL/animal group). In addition, the injection of DGP had no effect on general symptoms, weights, hematologic and serum biochemical examination, and necropsy. The results from the local tolerance tests at injection site showed no treatment related effects in the SD rats. Conclusion: The results of single dose intramuscular injection of DGP suggest that the approximate lethal dose is above 1.0 mL/animal for both male and female SD rats and that intramuscular injection of DGP may be safe.
Abstract
Objectives: The purpose of the study is to assess both the approximate lethal dose and the single dose intramuscular injection toxicity of Danggui (Angelica gigantis radix) pharmacopuncture (DGP) in Sprague-Dawley (SD) rats. Methods: The experiments were conducted at the good laboratory practice (GLP) laboratory, Biotoxtech Co., which is a laboratory approved by the ministry of food and drug safety (MFDS). The study was performed according to the GLP regulation and the toxicity test guidelines of the MFDS (2009) after approval of the institutional animal care and use committee of Biotoxtech. Single doses of DGP were injected intramuscularly into the rats in three test groups of 6 week old SD rats (5 male and 5 female rats per groups) in the amounts of 0.1, 0.5, and 1.0 mL/animal for groups 2, 3, and 4, respectively, and normal saline solution in the amount of 1.0 mL/animal was injected intramuscularly into the rats (5 male and 5 female rats) in the control group. Observations of the general symptoms and weight measurements were performed during the 14 day observation period after the injection. Hematologic and serum biochemical examination, necropsy, and a local tolerance test at the injection site were done after the observation period. Results: No death was observed in three test groups (0.1, 0.5 and 1.0 mL/animal group). In addition, the injection of DGP had no effect on general symptoms, weights, hematologic and serum biochemical examination, and necropsy. The results from the local tolerance tests at injection site showed no treatment related effects in the SD rats. Conclusion: The results of single dose intramuscular injection of DGP suggest that the approximate lethal dose is above 1.0 mL/animal for both male and female SD rats and that intramuscular injection of DGP may be safe.
참고문헌 (17)
- Korean Pharmacopuncture Insitute, "Pharmacopuncturology: principles and clinical application, Chapter 1, Definition and history", pp.3~4 (2012), Elsevier Korea LLC
- 박영철, "당귀(Angelica sinensis)의 임상활용에 대한 효능 및 부작용연구", 대한한의학 방제학회지, 19(2), pp.93~108 (2011), 대한한의학방제학회
- Han JH, "Korean traditional pharmacology,Chapter 20, Defiency-tonifying medicinal", pp.479~483 (2004), Euiseongdang publishing INC
- Kim YK, "Chemopreventive potential of Angelicae gigantis radix aqua-acupuncturesolution", Yakhak Hoeji, 44(3), pp.283~292 (2000)
- Park KH, "Studies on the analgesic effect of aqua-acupuncture with angelicae radix extract solution to mice", KyungHee Univ Orient Med J, 7, pp.261~271 (1984)
- Ahn JC, "Antioxidant effect of AGR (Angelicae girantis radix) aqua-acupuncture solution in vitro", The acupuncture, 13(2), pp.254~262 (1996)
- Hwang HS, "The effects of Angelicae gigantis radix aqua - acupuncture by density on immune response induced by radiation in mice", The acupuncture, 11(1), pp.113~129 (1994)
- Lee SJ, "Effects of aqua-acupuncture with saiviae radix and angelicae radix extract solution on the acute anemia induced by loss of blood in rats", KyungHee Univ Orient Med J, 14, pp.381~336 (1991)
- 박세환, "當歸 香氣液이 Rat의 뇌신경줄기세포의 分化와 增殖에 미치는 영향", 동의신경정신과학회지, 20(4), pp.63~77 (2009), 대한한방신경정신과학회
- 송봉근, "당귀 추출물 정맥 주사가 Middle Cerebral Artery Occlusion 모델 흰쥐에서 Gliosis 억제에 미치는 영향", Journal of Pharmacopuncture, 14(3), pp.5~18 (2011), 대한약침학회
- 한상균, "당귀약침의 혈해 자입이 Intraluminal Filament 삽입술에 의해 유발된 백서의 허혈성 뇌손상에 미치는 영향", 대한침구의학회지, 21(2), pp.1~20 (2004), 대한침구의학회
- Sun Y, "Water-soluble polysaccharides from Angelica sinensis (oliv.) diels: preparation, characterization and bioactivity", Int J Biol Macromol, 36(5), pp.283~289 (2005)
- Kim SJ, "Effects of radix Angelicae gigantis and resina ferulae on the relaxation of smooth muscle and expression of iNOS", J Korean Oriental Med, 20(2), pp.60~67 (2000)
- Kim YJ, "An experimental study on brain damage and cardiovascular system effect of Angelicae gigantis radix extract", J Korean Oriental Med, 21(4), pp.37~47 (2000)
- Kim KS, "Effects of aqua-acupuncture with radix Angelicae gigantis and radix astragali on the blood in the liver damage rats with CCL4", The acupuncture, 13(1), pp.1~10 (1996)
- Yan R, "Pharmacokinetics and metabolism of ligustilide, a major bioactive component in rhizoma chuanxiong, in the rat", Drug Metab Dispos, 36(2), pp.400~408 (2008)
- Barlow S, "Risk assessment of carcinogens in food", Toxicol Appl Pharmacol, 243(2), pp.180~190 (2010)