영상 타원법을 이용한 인간 줄기세포의 굴절률과 두께 분포 연구
Study on Refractive Index and Thickness of Human Stem Cells by Using Imaging Ellipsometry
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초록
타원법을 사용하여 인간 중간엽 줄기세포의 광학상수와 두께의 영상정보를 정량적으로 구하였다. 영상 타원계를 이용하여 in vitro 환경에서 세포의 핵(nucleus)과 세포체(cell body)의 타원상수 , 를 2차원 영상정보 형태로 구한 다음 각 화소별로 타원상수들을 수치해석적 역방계산하여 굴절률과 두께의 영상정보를 얻었다. 세포의 핵 영역과 세포체 영역에서 줄기세포의 두께와 굴절률 값을 2차원 영상정보 형태로 제시하였다.
Abstract
We applied an ellipsometric technique to get quantitative information about the thickness and refractive index of human Mesenchymal Stem Cells (hMSCs). The images of ellipsometric constants , for the nucleus region and for the cell body region of hMSCs were obtained by using an Imaging Ellipsomter (IE) for their in vitro state. A numerical inversion method was applied to deduce the refractive index and the thickness of hMSCs from the measured , . Thus the images of the refractive index and those of the thickness of hMSCs for the nucleus region and for the cell body region are reported.
참고문헌 (10)
- 김상열, "타원법", pp.115~120 (2000), 아주대학교 출판부
- 안일신, "엘립소미트리", pp.177~181 (2000), 한양대학교 출판부
- H. G. Tompkins, "Handbook of Ellipsometry", pp.821~825 (2005), William Andrew
- G. Jin, "Imaging Ellipsometry for the Visualization of Bio-molecular Layers", Engineering in Medicine and Biology Society Proceedings of the 20th Annual International Conference of the IEEE, 20(2), pp.581~584 (1998)
- R. Seitz, "Protein adsorption on solid–liquid interfaces monitored by laser-ellipsometry", Applied Surface Science, 252(1), pp.154~157 (2005)
- M. Poksinski, "Protein monolayers monitored by internal reflection ellipsometry", The 3rd International Conference on Spectroscopic Ellipsometry, 455, pp.716~721 (2004)
- A. Joiner, "Ellipsometry analysis of the in vitro adsorption of tea polyphenols onto salivary pellicles", European Journal of Oral Sciences, 112(6), pp.510~515 (2004)
- G. Cox, "Optical Imaging Techniques in Cell Biology", pp.233~238 (2007), CRC Press
- Y. Katsube, "Correlation between proliferative activity and cellular thickness of human mesenchymal stem cells", Biochemical and Biophysical Research Communications, 368(2), pp.256~260 (2008)
- F. L. McCrackin, "Measurement of the Thickness and Refractive Index of Very Thin Films and the Optical Properties of Surfaces by Ellipsometry", J.Res.Nat.Bur.Standards-A.physics and Chemistry, 67A(4), pp.363~377 (1963)