Diagnostic efficacy of a recombinant cysteine protease of Spirometra erinacei larvae for serodiagnosis of sparganosis
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The mature domain of a cysteine protease of Spirometra erinacei plerocercoid larva (i.e., sparganum) was expressed in Escherichia coli, and its value as an antigen for the serodiagnosis of sparganosis was investigated. The recombinant protein (rSepCp-1) has the molecular weight of 23.4 kDa, and strongly reacted with the sparganum positive human or mice sera but not with negative sera by immunoblotting. ELISA with rSepCp-1 protein or sparganum crude antigen (SeC) was evaluated for the serodiagnosis of sparganosis using patient's sera. The sensitivity and specificity of ELISA using rSepCp-1 protein were 95.0% (19/20) and 99.1% (111/112), respectively. In contrast, the sensitivity and specificity of ELISA with SeC were 100% (20/20) and 96.4% (108/112), respectively. Moreover, in experimentally infected mice, the sensitivity and specificity of both ELISA assays were 100% for the detection of anti-sparganum IgG. It is suggested that the rSepCp-1 protein-based ELISA could provide a highly sensitive and specific assay for the diagnosis of sparganosis.
Abstract
The mature domain of a cysteine protease of Spirometra erinacei plerocercoid larva (i.e., sparganum) was expressed in Escherichia coli, and its value as an antigen for the serodiagnosis of sparganosis was investigated. The recombinant protein (rSepCp-1) has the molecular weight of 23.4 kDa, and strongly reacted with the sparganum positive human or mice sera but not with negative sera by immunoblotting. ELISA with rSepCp-1 protein or sparganum crude antigen (SeC) was evaluated for the serodiagnosis of sparganosis using patient's sera. The sensitivity and specificity of ELISA using rSepCp-1 protein were 95.0% (19/20) and 99.1% (111/112), respectively. In contrast, the sensitivity and specificity of ELISA with SeC were 100% (20/20) and 96.4% (108/112), respectively. Moreover, in experimentally infected mice, the sensitivity and specificity of both ELISA assays were 100% for the detection of anti-sparganum IgG. It is suggested that the rSepCp-1 protein-based ELISA could provide a highly sensitive and specific assay for the diagnosis of sparganosis.
참고문헌 (21)
- Li MW, "Sparganosis in mainland China", Int J Inf Dis, 15, pp.e154~e156 (2011)
- Jin Hoon Park, "Sparganosis in the Lumbar Spine : Report of Two Cases and Review of the Literature", 대한신경외과학회지, 49(4), pp.241~244 (2011), 대한신경외과학회
- Nithiuthai S, "Waterborne zoonotic helminthiases", Vet Parasitol, 126, pp.167~193 (2004)
- Kwon JH, "Sparganosis presenting as a conus medullaris lesion : case report and literature review of the spinal sparganosis", Arch Neurol, 61, pp.1126~1128 (2004)
- Lee JH, "Abdominal sparganosis presenting as an abscess with fistulous communication to the bowel", Am J Roentgenol, 185, pp.1084~1085 (2005)
- Song T, "CT and MR characteristics of cerebral sparganosis", Am J Neuroradiol, 28, pp.1700~1705 (2007)
- 구자현, "A case of sparganosis mimicking a varicose vein", The Korean Journal of Parasitology, 44(1), pp.91~94 (2006), 대한기생충학ㆍ열대의학회
- Chae SW, "Sparganosis presenting as a lateral neck mass", Head Neck, 25, pp.74~76 (2003)
- Kong Y, "Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography", Korean J Parasitol, 29, pp.1~7 (1991)
- Cui J, "Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana", Parasitol Res, 108, pp.1551~1556 (2011)
- McKerrow JH, "Proteases in parasitic diseases", Annu Rev Pathol, 1, pp.497~536 (2006)
- El-Sayed LH, "Diagnostic significance of Schistosoma mansoni proteins Sm31 and Sm32 in human schistosomiasis in an endemic area in Egypt", Trop Med Int Health, 3, pp.721~727 (1998)
- Carnevale S, "Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase", Diagn Microbiol Infect Dis, 41, pp.43~49 (2001)
- Nagano I, "Molecular expression of a cysteine proteinase of Clonorchis sinensis and its application to an enzyme-linked immunosorbent assay for immunodiagnosis of clonorchiasis", Clin Diagn Lab Immunol, 11, pp.411~416 (2004)
- Ikeda T, "Enzyme-linked immunosorbent assay using cysteine proteinase antigens for immunodiagnosis of human paragonimiasis", Am J Trop Med Hyg, 55, pp.435~437 (1996)
- Kong Y, "A neutral cysteine protease of Spirometra mansoni plerocercoid invoking an IgE response", Parasitology, 114, pp.263~271 (1997)
- Liu DW, "Molecular cloning and expression of the gene encoding a cysteine proteinase of Spirometra erinacei", Mol Biochem Parasitol, 76, pp.11~21 (1996)
- Yang SH, "Cloning and characterization of a new cysteine proteinase secreted by Paragonimus westermani adult worms", Am J Trop Med Hyg, 71, pp.87~92 (2004)
- Rafati S, "Expression of cysteine proteinase type I and II of Leishmania infantum and their recognition by sera during canine and human visceral leishmaniasis", Exp Parasitol, 103, pp.143~151 (2003)
- Matthaiou DK, "Albendazole versus praziquantel in the treatment of neurocysticercosis: a metaanalysis of comparative trials", PLoS Negl Trop Dis, 2, pp.e194~ (2008)
- Kim DG, "Cerebral sparganosis : clinical manifestations, treatment, and outcome", J Neurosurg, 85, pp.1066~1071 (1996)