Partial Purification and Characterization of a Cysteine Protease Inhibitor from the Plerocercoid of Spirometra erinacei
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Helminthic cysteine proteases are well known to play critical roles in tissue invasion, nutrient uptake, and immune evasion of the parasites. In the same manner, the sparganum, the plerocercoid of Spirometra mansoni, is also known to secrete a large amount of cysteine proteases. However, cysteine protease inhibitors regulating the proteolytic activities of the cysteine protease are poorly illustrated. In this regard, we partially purified an endogenous cysteine protease inhibitor from spargana and characterized its biochemical properties. The cysteine protease inhibitor was purified by sequential chromatographies using Resource Q anion exchanger and Superdex 200 HR gel filtration from crude extracts of spargana. The molecular weight of the purified protein was estimated to be about 11 kD on SDS-PAGE. It was able to inhibit papain and 27 kDa cysteine protease of spargana with the ratio of 25.7% and 49.1%, respectively, while did not inhibit chymotrypsin. This finding suggests that the cysteine protease inhibitor of spargana may be involved in regulation of endogenous cysteine proteases of the parasite, rather than interact with cysteine proteases from their hosts.
Abstract
Helminthic cysteine proteases are well known to play critical roles in tissue invasion, nutrient uptake, and immune evasion of the parasites. In the same manner, the sparganum, the plerocercoid of Spirometra mansoni, is also known to secrete a large amount of cysteine proteases. However, cysteine protease inhibitors regulating the proteolytic activities of the cysteine protease are poorly illustrated. In this regard, we partially purified an endogenous cysteine protease inhibitor from spargana and characterized its biochemical properties. The cysteine protease inhibitor was purified by sequential chromatographies using Resource Q anion exchanger and Superdex 200 HR gel filtration from crude extracts of spargana. The molecular weight of the purified protein was estimated to be about 11 kD on SDS-PAGE. It was able to inhibit papain and 27 kDa cysteine protease of spargana with the ratio of 25.7% and 49.1%, respectively, while did not inhibit chymotrypsin. This finding suggests that the cysteine protease inhibitor of spargana may be involved in regulation of endogenous cysteine proteases of the parasite, rather than interact with cysteine proteases from their hosts.
참고문헌 (16)
- Cho SY, "Some aspects of human sparganosis", The Korean Journal of Parasitology, 13(1), pp.60~77 (1975), 대한기생충학회
- Fukase T, "Purification and some properties of cysteine protease of Spirometra erinacei plerocercoid (Cestoda: Diphyllobothriidae)", Japanese Journal of Parasitology, 34, pp.351~360 (1985)
- Song CY, "Purification and partial characterization of cysteine proteinase from Spirometra mansoni plerocercoids", JOURNAL OF PARASITOLOGY, 79, pp.517~524 (1993)
- Kong Y, "Cleavage of immunoglobulin G by excretory-secretory cathepsin S-like protease of Spirometra mansoni plerocercoid", Parasitology, 109, pp.611~621 (1994)
- Kong Y, "A neutral cysteine protease of Spirometra mansoni plerocercoid invoking an IgE response", Parasitology, 114, pp.263~271 (1997)
- Sajid M, "Cysteine proteases of parasitic organisms", Molecular and Biochemical Parasitology, 120, pp.1~21 (2002)
- Morris MT, "Functional analysis of Toxoplasma gondii protease inhibitor 1", JOURNAL OF BIOLOGICAL CHEMISTRY, 277, pp.45259~45266 (2002)
- Lustigman S, "Molecular cloning and characterization of onchocystatin, a cysteine proteinase inhibitor of Onchocerca volvulus", JOURNAL OF BIOLOGICAL CHEMISTRY, 267, pp.17339~17346 (1992)
- Newlands GFJ, "Cloning and expression of cystatin, a potent cysteine protease inhibitor from the gut of Haemonchus contortus", Parasitology, 122, pp.371~378 (2001)
- Murray J, "Bm-CPI-2, a cystatin from Brugia malayi nematode parasites, differs from Caenorhabditis elegans cystatins in a specific site mediating inhibition of the antigen-processing enzyme AEP", Molecular and Biochemical Parasitology, 139, pp.197~203 (2005)
- Khanznadji E, "A new multi-domain member of the cystatin superfamily expressed by Fasciola hepatica", INTERNATIONAL JOURNAL FOR PARASITOLOGY, 35, pp.1115~1125 (2005)
- Chung YB, "Activities of different cysteine proteases of Paragonimus westermani in cleaving human IgG", The Korean Journal of Parasitology, 35(2), pp.139~142 (1997), 대한기생충학회
- Barrett AJ, "The cystatins: a diverse superfamily of cysteine peptidase inhibitors", Biomedica Biochimica Acta, 45, pp.1363~1374 (1986)
- 김지영, "Partial characterization of a 29 kDa cysteine protease purified from Taenia solium metacestodes", The Korean Journal of Parasitology, 43(4), pp.157~160 (2005), 대한기생충학회
- Abrahamson M, "Cystatins", Methods in Enzymology, 244, pp.685~700 (1994)
- Morales FC, "The N-terminus moiety of the cystatin SmCys from Schistosoma mansoni regulates its inhibitory activity in vitro and in vivo", Molecular and Biochemical Parasitology, 134, pp.65~73 (2004)