Increased 26S proteasome non-ATPase regulatory subunit 1 in the aqueous humor of patients with age-related macular degeneration
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Age-related macular degeneration (AMD) is the leading causeof blindness in the world. Evidence indicates that thesuppression of the ubiquitin-proteasome system (UPS) contributesto the accumulation of toxic proteins and inflammation inretinal pigment epithelium (RPE), the functional abnormalitiesand/or the degeneration of which are believed to be theinitiators and major pathologies of AMD. To identify newprotein associations with the altered UPS in AMD, we usedLC-ESI-MS/MS to perform a proteomic analysis of the aqueoushumor (AH) of AMD patients and matched control subjects. SixUPS-related proteins were present in the AH of the patients andcontrol subjects. Four of the proteins, including 26S proteasomenon-ATPase regulatory subunit 1 (Rpn2), were increasedin patients, according to semi-quantitative proteomic profiling. An LC-MRM assay revealed a significant increase of Rpn2 in 15AMD patients compared to the control subjects, suggesting thatthis protein could be a biomarker for AMD.
Abstract
Age-related macular degeneration (AMD) is the leading causeof blindness in the world. Evidence indicates that thesuppression of the ubiquitin-proteasome system (UPS) contributesto the accumulation of toxic proteins and inflammation inretinal pigment epithelium (RPE), the functional abnormalitiesand/or the degeneration of which are believed to be theinitiators and major pathologies of AMD. To identify newprotein associations with the altered UPS in AMD, we usedLC-ESI-MS/MS to perform a proteomic analysis of the aqueoushumor (AH) of AMD patients and matched control subjects. SixUPS-related proteins were present in the AH of the patients andcontrol subjects. Four of the proteins, including 26S proteasomenon-ATPase regulatory subunit 1 (Rpn2), were increasedin patients, according to semi-quantitative proteomic profiling. An LC-MRM assay revealed a significant increase of Rpn2 in 15AMD patients compared to the control subjects, suggesting thatthis protein could be a biomarker for AMD.
참고문헌 (33)
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