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대전지역의 3차 병원에서 분리된 Carbapenem 내성Pseudomonas aeruginosa의 병독성 인자 검출

Molecular Detection of Virulence Factors in Carbapenem-Resistant Pseudomonas aeruginosa Isolated from a Tertiary Hospital in Daejeon

조혜현
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)|2019.09|대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)|제51권 제3호|pp. 301-308|DOI 10.15324/kjcls.2019.51.3.301

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키워드

Virulence factorMultidrug-resistantExoU geneVirulence facto
주제분류기타자연과학

초록

다제내성 P. aeruginosa의 출현과 확산은 전 세계적으로 중요한 문제가 되고 있다. P. aeruginosa에 의한 발병은 일부 몇몇세포 관련 및 세포외 병독성 인자의 생성에 기인한다. 본 연구에서는 대전지역의 3차 병원에서 분리된 carbapenem 내성 P.aeruginosa를 대상으로 병독성 인자의 분포와 항균제 내성 양상을 조사하였다. 항균제 감수성 시험은 디스크 확산법으로 확인하였고, 병독성 유전자의 분석을 위해 PCR과 염기서열분석을 수행하였다. 또한, 다제내성 P. aeruginosa의 sequence type (ST)은 multilocus sequence typing (MLST)을 통해 확인하였다. 32균주의 carbapenem 내성 P. aeruginosa 중, 14균주(43.8%)가 다제내성이었으며, 주요 ST는 ST235 (10균주, 71.4.%)임을 확인하였다. 병독성 유전자는 32균주 모두에서 확인되었고, 이 중 가장 높은 빈도로 확인된 병독성 유전자는toxA, plcN, phzM (100.%)이었다. 또한, 32균주는 모두 8개 이상의 병독성 유전자를 가지고 있었으며, 9균주(28.1%)가 15개의 병독성 유전자를 가지고 있었다. exoU 유전자는 다제내성 P.aeruginosa 균주의 71.4%에서 확인되었다. 이러한 결과는exoU 유전자가 다제내성 P. aeruginosa 균주의 지속성에 대한예측 표지자가 될 수 있을 것으로 사료된다.

Abstract

The emergence and spread of multidrug resistant (MDR) Pseudomonas aeruginosa is a critical problem worldwide. The pathogenesis of P. aeruginosa is due partly to the production of several cell-associated and extracellular virulence factors. This study examined the distribution of virulence factors and antimicrobial resistance patterns of carbapenem-resistant P. aeruginosa (CRPA) isolated from a tertiary hospital in Daejeon, Korea. Antimicrobial susceptibility testing was performed using the disk diffusion method, and PCR and DNA sequencing were performed to determine for the presence of virulence genes. In addition, the sequence type (ST) of MDR P.aeruginosa was investigated by multilocus sequence typing (MLST). Among 32 CRPA isolates, 14 (43.8%) were MDR and the major ST was ST235 (10 isolates, 71.4%). All isolates were positive for the presence of virulence genes and the most prevalent virulence genes were toxA, plcN, and phzM (100%). All isolates carried at least eight or more different virulence genes and nine (28.1%) isolates had 15 virulence genes. The presence of the exoU gene was detected in 71.4% of the MDR P. aeruginosa isolates. These results indicate that the presence of the exoU gene can be a predictive marker for the persistence of MDR P. aeruginosa isolates.

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관련 논문AI추천

대전지역의 입원환자에서 분리된 Carbapenem 내성 Pseudomonas aeruginosa의 분자역학조사(2008년에서 2014년까지)조혜현대한임상검사과학회 · 대한임상검사과학회지2018 · 제50권 제4호Characteristics of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa in Korea김정옥, 배일권, 이경원, 이혁민, 정석훈, 홍준성대한감염학회 · Infection and Chemotherapy2015 · 제47권 제1호Genetic Epidemiology and Antimicrobial Susceptibilities of Carbapenem-Resistant Pseudomonas aeruginosa Isolates from a Multicenter Study in KoreaChoi Tae Youn, Choi Young Jin, Chong Yong Pil, Cho Oh-Hyun, Hong Yoon-Kyoung, Jung Jongtak, Kim Mi-Na, Kim Tark, Park Sunghee, Shin Hee Bong대한감염학회 · Infection and Chemotherapy2025 · 제57권 제3호Correlation Between Virulence Genotype and Fluoroquinolone Resistance in Carbapenem-Resistant Pseudomonas aeruginosa구선회, 권계철, 김세미, 조혜현대한진단검사의학회 · Annals of Laboratory Medicine2014 · 제34권 제4호Coexistence of Multidrug Resistance and Hypervirulence-Associated Genes in Clinical Carbapenem-Resistant Pseudomonas aeruginosaCho Hye Hyun, Park Yumi한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Distribution of Pseudomonas-Derived Cephalosporinase and Metallo-β-Lactamases in Carbapenem-Resistant Pseudomonas aeruginosa Isolates from Korea구선회, 권계철, 김세미, 조혜현한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2015 · 제25권 제7호

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