HPLC/DAD/ESI-MS를 이용한 혈장시료 중 갑상선 호르몬 분석

Abstract

An analytical method for the determination of thyroid hormones in plasma samples has been studiedby solid-phase extraction and high-performance liquid chromatography/diode array detector (DAD)/electrosprayon the reverse phase Hypersil ODS column (4.6 mm I.D., 250 mm length, particle size 5 m) with amoniumformate buffer and acetonitrile. In addition, these compounds were confirmed by UV spectra and ESI-massSpectra. The extraction recoveries of thyroid hormones in the plasma sample (at pH 3) were in the range of74.5-15.7 % with solid-phase extraction by C18, followed by elution with 4.mL of methanol. The calibration. Corresponding authorPhone : +82-(0)2-958-5181 Fax : +82-(0)2-958-5059E-mail : phs3692@kist.re.kr425 ...........Analytical Science & Technologycurves showed good linearity with the correlation coefficients (r2) varying from 0.9939 to 0.9978 and thedetection limits of all analytes were obtained in the range of 20-50 ng/mL (38.1-162.8 pmol/mL). As a result,thyroxine was found in the range of 50.98-112.97 ng/mL in normal plasma samples.