Forensic Application of Age Estimation Using Signal Joint T-Cell Receptor Excision Circles in Human Lymphoid Tissues
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The declining tendency of signal joint T-cell receptor excision circles (sjTRECs) in peripheral blood is known to be age-dependent, and their quantification in blood or bloodstains has recently been introduced as a tool for age estimation. Lymphoid tissues such as the thymus and spleen represent potential candidates for age estimation because they undergo age-related structural and functional changes. In the present study, the correlation between age and sjTREC levels in human lymphoid tissues, namely the thymus, spleen, and blood, obtained from autopsy cases were investigated, with the goal of establishing a reliable age estimation model. Results showed negative regression curves with coefficient values of r=-0.410, r=-0.611, and r=-0.584 for thymus, spleen, and blood, respectively. In addition, this model was testing using thymus samples from the torsos of dismembered bodies from two real forensic cases, and results showed the predicted ages to be close to the actual ages of the victims. Further study will be required to improve accuracy and reduce estimation error, particularly within the lower age range. Nonetheless, these results suggest that quantification of sjTRECs in not only blood but also in other lymphoid tissues could be a useful tool for age estimation in forensic cases.
Abstract
The declining tendency of signal joint T-cell receptor excision circles (sjTRECs) in peripheral blood is known to be age-dependent, and their quantification in blood or bloodstains has recently been introduced as a tool for age estimation. Lymphoid tissues such as the thymus and spleen represent potential candidates for age estimation because they undergo age-related structural and functional changes. In the present study, the correlation between age and sjTREC levels in human lymphoid tissues, namely the thymus, spleen, and blood, obtained from autopsy cases were investigated, with the goal of establishing a reliable age estimation model. Results showed negative regression curves with coefficient values of r=-0.410, r=-0.611, and r=-0.584 for thymus, spleen, and blood, respectively. In addition, this model was testing using thymus samples from the torsos of dismembered bodies from two real forensic cases, and results showed the predicted ages to be close to the actual ages of the victims. Further study will be required to improve accuracy and reduce estimation error, particularly within the lower age range. Nonetheless, these results suggest that quantification of sjTRECs in not only blood but also in other lymphoid tissues could be a useful tool for age estimation in forensic cases.
참고문헌 (9)
- Lee SS, "The chronology of second and third molar development in Koreans and its application to forensic age estimation", Int J Legal Med, 124, pp.659~665 (2010)
- Bocklandt S, "Epigenetic predictor of age", PLoS One, 6, pp.e14821~ (2011)
- Zubakov D, "Estimating human age from T-cell DNA rearrangements", Curr Biol, 20, pp.R970~R971 (2010)
- Ou XL, "Predicting human age with bloodstains by sjTREC quantification", PLoS One, 7, pp.e42412~ (2012)
- Strobel P, "The ageing and myasthenic thymus: a morphometric study validating a standard procedure in the histological workup of thymic specimens", J Neuroimmunol, 201-202, pp.64~73 (2008)
- Zhang L, "Measuring recent thymic emigrants in blood of normal and HIV-1-infected individuals before and after effective therapy", J Exp Med, 190, pp.725~732 (1999)
- Cho S, "Age estimation via quantification of signal-joint T cell receptor excision circles in Koreans", Leg Med (Tokyo), 16, pp.135~138 (2014)
- Jamieson BD, "Generation of functional thymocytes in the human adult", Immunity, 10, pp.569~575 (1999)
- Douek DC, "Changes in thymic function with age and during the treatment of HIV infection", Nature, 396, pp.690~695 (1998)