갈근과 두시 복합추출물의 아토피피부염 유발 백서에서의 피부장벽 손상 회복 효과

Abstract

Objective This study aimed to evaluate the effect of a combined extract of Puerariae Radix (葛根) and fermented soybean (Douchi, 豆豉) on the recovery of skin barrier damage in atopic dermatitis (AD). Methods AD was induced in four-week-old NC/Nga mice, after which the combined extract of Puerariae Radix and Douchi was administered. To assess the skin barrier recovery through the generation of moisturizing factors, ceramide kinase and Caspase 14 were observed. To evaluate antimicrobial barrier recovery, Na⁺/H⁺ exchanger (NHE) and cathelicidin were examined. Cclaudin and Toll-like receptor 2 (TLR-2) were analyzed to assess tight junction recovery. Results Positive expression of ceramide kinase was higher in the Puerariae Radix and Douchi complex extract administration group after lipid barrier elimination (PDET) than in the lipid barrier eliminated group (LBE) and ceramide exposure group after lipid barrier elimination (CERT); caspase 14 expression showed less reduction in the PDET group. NHE expression was reduced to a lesser extent in the PDET group than that in the LBE and CERT groups, whereas cathelicidin expression was more increased in the PDET group. Claudin expression was also higher in the PDET group than in the LBE and CERT groups, and TLR-2 expression was significantly more increased in the PDET group. Conclusion The combined extract of Puerariae Radix and Douchi has the potential to restore skin barrier damage in atopic dermatitis.