Recovery of Streptococcus Mutans Biofilm after Photodynamic Therapy with Erythrosine and LED Light Source
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The aim of this study was to evaluate the effects of erythrosine-mediated photodynamic therapy (PDT) on Streptococcus mutans biofilm recovery by counting its colony-forming units (CFUs) and via confocal laser scanning microscopy analysis at different time points following PDT. In PDT, photosensitizer was an erythrosine. S. mutans ATCC25175 biofilms were irradiated using an LED curing light. Chlorhexidine (CHX) was used as positive control. After each antimicrobial treatment, samples were cultured to allow biofilm recovery. Viability was measured by calculating the CFU counts after treatment and after every 3 hours for up to 24 hours. Immediately after treatment, the PDT and CHX groups showed equally significant decreases in S. mutans CFU counts compared to the negative control. After 12 hours of reculture, the PDT group showed no significant difference in the decrease in CFU count compared to the negative control, whereas the CHX group showed significantly lower CFU counts throughout the 24-hour period. Erythrosine-mediated PDT can effectively inhibit S. mutans biofilm formation. However, biofilm recovery occurred earlier in the CHX group after PDT. This study provides insights into the clinical effectiveness of PDT in preventing dental caries.
Abstract
The aim of this study was to evaluate the effects of erythrosine-mediated photodynamic therapy (PDT) on Streptococcus mutans biofilm recovery by counting its colony-forming units (CFUs) and via confocal laser scanning microscopy analysis at different time points following PDT. In PDT, photosensitizer was an erythrosine. S. mutans ATCC25175 biofilms were irradiated using an LED curing light. Chlorhexidine (CHX) was used as positive control. After each antimicrobial treatment, samples were cultured to allow biofilm recovery. Viability was measured by calculating the CFU counts after treatment and after every 3 hours for up to 24 hours. Immediately after treatment, the PDT and CHX groups showed equally significant decreases in S. mutans CFU counts compared to the negative control. After 12 hours of reculture, the PDT group showed no significant difference in the decrease in CFU count compared to the negative control, whereas the CHX group showed significantly lower CFU counts throughout the 24-hour period. Erythrosine-mediated PDT can effectively inhibit S. mutans biofilm formation. However, biofilm recovery occurred earlier in the CHX group after PDT. This study provides insights into the clinical effectiveness of PDT in preventing dental caries.
참고문헌 (28)
- Jiao Y, "Advancing antimicrobial strategies for managing oral biofilm infections", Int J Oral Sci, 11, pp.1~11 (2019)
- Alves-Barroco C, "Singularities of pyogenic Streptococcal biofilms-from formation to health implication", Front Microbiol, 11, pp.584947~ (2020)
- Berger D, "Oral biofilms : Development, control, and analysis", High Throughput, 7, pp.24~ (2018)
- de Carvalho FG, "Presence of mutans streptococci and Candida spp. in dental plaque", dentine of carious teeth and early childhood caries, 2006, pp.51, 1024~1027
- Banas JA, "Virulence properties of Streptococcus mutans", Front Biosci, 9, pp.1267~1277 (2004)
- Rajesh S, "Antimicrobial photodynamic therapy : An overview", J Indian Soc Periodontol, 15, pp.323~327 (2011)
- Kim CK, "Effects of the C31G, Listerine and CPC as a adjunctives to the mechanical plaque control on the early periodontitis", J Periodontal Implant Sci, 28, pp.631~646 (1998)
- Takasaki AA, "Application of antimicrobial photodynamic therapy in periodontal and periimplant diseases", Periodontol 2000, 51, pp.109~140 (2009)
- Wood S, "Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms", J Antimicrob Chemother, 57, pp.680~684 (2006)
- Costa ACBP, "The effects of rose bengal-and erythrosine-mediated photodynamic therapy on Candida albicans", Mycoses, 55, pp.56~63 (2012)
- 김재용, "Antimicrobial Effect on Streptococcus mutans in Photodynamic Therapy using Different Light Source", 대한소아치과학회지, 45(1), pp.82~89 (2018), 대한소아치과학회[DOI]
- 정지숙, "THE EFFECT OF PHOTODYNAMIC THERAPY ON THE VIABILITY OF STREPTOCOCCUS MUTANS ISOLATED FROM ORAL CAVITY", 대한소아치과학회지, 39(3), pp.233~241 (2012), 대한소아치과학회[DOI]
- 박초아, "Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach", 대한소아치과학회지, 47(1), pp.70~77 (2020), 대한소아치과학회[DOI]
- 최서정, "Streptococcus mutans biofilm에 대한 광역동 치료의 최적조건에 관한 연구", 대한소아치과학회지, 42(2), pp.151~157 (2015), 대한소아치과학회[DOI]
- 고준희, "치면착색제를 이용한 광역동 치료의 항균 효과", 대한소아치과학회지, 47(2), pp.120~127 (2020), 대한소아치과학회[DOI]
- Cieplik F, "Antimicrobial photodynamic therapy for inactivation of biofilms formed by oral key pathogens", Front Microbiol, 5, pp.405~ (2014)
- Imfeld T, "Chlorhexidine-containing chewing gum", Schweiz Monatsschr Zahnmed, 116, pp.476~483 (2006)
- Bonesvoll P, "Oral pharmacology of chlorhexidine", J Clin Periodontol, 4, pp.49~65 (1977)
- Varoni E, "Chlorhexidine(CHX)in dentistry : state of the art", Minerva Stomatol, 61, pp.399~419 (2012)
- Cieplik F, "Resistance toward chlorhexidine in oral bacteria-Is there cause for concern?", Front Microbiol, 10, pp.587~ (2019)
- Tavares A, "Antimicrobial photodynamic therapy : study of bacterial recovery viability and potential development of resistance after treatment", Mar Drugs, 8, pp.91~105 (2010)
- Goulart RDC, "Photodynamic therapy in planktonic and biofilm cultures of Aggregatibacter actinomycetemcomitans", Photomed Laser Surg, 28, pp.53~60 (2010)
- Konopka K, "Photodynamic therapy in dentistry", J Dent Res, 86, pp.694~707 (2007)
- Stájer A, "Utility of photodynamic therapy in dentistry : Current concepts", Dent J, 8, pp.43~ (2020)
- Trowbridge HO, "Sensory response to thermal stimulation in human teeth", J Endod, 6, pp.405~412 (1980)
- Zach L, "Pulp response to externally applied heat", Oral Surg Oral Med Oral Pathol, 19, pp.515~530 (1965)
- De Freitas MTM, "Effect of photodynamic antimicrobial chemotherapy on mono-and multi-species cariogenic biofilms : a literature review", Photomed Laser Surg, 35, pp.239~245 (2017)
- Prazmo EJ, "Photodynamic therapy as a promising method used in the treatment of oral diseases", Adv Clin Exp Med, 25, pp.799~807 (2016)