Protective Effects of Epigallocatechin Gallate after UV Irradiation in Cultured Human Retinal Pigment Epithelial Cells
2
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
Purpose: To evaluate the protective effects of Epigallocatechin gallate (EGCG) against UV irradiation in cultured human retinal pigment epithelial (RPE) cells. Methods: UV irradiation was produced by a UV lamp for 30 seconds with an irradiance of 3.3 mW/cm2. After 5 minutes and 1 hour, we administered different concentrations of EGCG (0, 5, 10, 15, 25, 50, 100 uM). The cell count was determined under a microscope using a counting chamber and the cell activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: The cell count of cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group, compared with the non-administrated group. The cell activity of the cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group and was increased in a dose-dependent way as determined by the MTT assay. Conclusions: The administration of EGCG increased the cell count and the cell activity after UV irradiation in cultured human retinal pigment epithelial cells; this suggests that EGCG provided protection against UV damage in cultured human retinal pigmented epithelial cells.
참고문헌 (41)
- McCarty CA, "Recent developments in vision research : light damage in cataract", 37, pp.1720~1723 (1996)
- Norren DV, "Spectral transmission of the human ocular media", (rren dv), pp.1237~44
- Margrain TH, "Do blue light filters confer protection against age-related macular degeneration", 23, pp.523~531 (2004)
- Patton WP, "Comet assay of UV-induced DNA damage in retinal pigment epithelial cells", 40, pp.3268~3275 (1999)
- Mainster MA, "and rationale of ultraviolet-protective intraocular lenses. Am J Ophthalmol 1986", pp.727~32
- Sujak A, "Lutein and zeaxanthin as protectors of lipid membranes against oxidative damage: The structural aspects", 371, pp.301~307 (1997)
- Sujak A, "Organisation of xanthophyll pigment lutein and zeaxanthin in lipid membranes formed with dipalmitoylphosphatidylcholine", 1509, pp.255~263 (2000)
- Yu HG, "Oxidative Damage induced by UV in Cultured Retinal Pigment Epithelial Cells", 44, pp.2890~2895 (2003)
- "Van Kuijk FJ. Effect of ultraviolet light on the eye role of protective glasses. Environ Health Perspet 1991", pp.177~84
- Noell WK, "Berman S. Retinal damage by light in rats. Invest Ophthalmol Vis Sci 1966", pp.450~73
- Beatty S, "The role of oxidative stress in the pathogenesis of age-related macular degeneration", 45, pp.115~134 (2000)
- "A randomized, placebo-controlled, clinical trial of high-dose supplementation with vitamins C and E, beta carotene, and zinc for age-related macular degeneration and vision loss", 119, pp.1417~1436 (2001)
- Seddon JM, "Dietary carotenoids, vitamins A, C, and E, and advanced age-related macular degeneration", 272, pp.1413~1420 (1995)
- Hanneken A, "Flavonoids Protect Human Retinal Pigment Epithelial Cells from Oxidative-Stress-Induced Death", 47, pp.3164~3177 (2006)
- Middleton E Jr, "The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease and cancer", 52, pp.673~751 (2000)
- Wang YC, "The specific anti-cancer activity of green tea (-)-epigallocatechin-3-gallate (EGCG)", 22, pp.131~143 (2002)
- Kimura M, "The relation between single", double or repeated tea catechin ingestions and plasma antioxidant activity in humans, 2002, pp.56, 1186~1193
- Buttemeyer R, "Epigallocatechin gallate can significantly decrease free oxygen radicals in the reperfusion injury in vivo", 35, pp.3116~3120 (2003)
- Morley N, "The green tea polyphenol ( )-epigallocatechin gallate and green tea can protect human cellular DNA from ultraviolet and visible radiation-induced damage", 21, pp.15~22 (2005)
- Nagai K, "( )-Epigallocatechin gallate protects against NO stress-induced neuronal damage after ischemia by acting as an anti-oxidant", 956, pp.319~322 (2002)
- Xie D, "( )-Epigallocatechin-3-gallate protects cultured spiral ganglion cells from H2O2-induced oxidizing damage", 124, pp.464~470 (2004)
- Mandel S, "Cell signaling pathways in the neuroprotective actions of the green tea polyphenol ( )-epigallocatechin-3-gallate : implications for neurodegenerative diseases", 88, pp.1555~1569 (2004)
- Skrzydlewska E, "Protective effect of green tea against lipid peroxidation in the rat liver, blood serum and the brain", 9, pp.232~238 (2002)
- Yokozawa T, "Antioxidative activity of green tea polyphenol in cholesterol-fed rats", 50, pp.3549~3552 (2002)
- Negishi H, "Black and green tea polyphenols attenuate blood pressure increases in stroke- prone spontaneously hypertensive rats", 134, pp.38~42 (2004)
- Sutherland BA, "Appleton. Mechanisms of action of green tea catechins, with a focus on ischemia- induced neurodegeneration", 17, pp.291~306 (2006)
- Chung JH, "Dual mechanisms of green tea extract (EGCG) induced cell survival in human epidermal keratinocytes", 17, pp.1913~1915 (2003)
- Zhang B, "Oxidative-induced retinal degeneration is attenuated by Epigallocatechin gallate", 1124, pp.176~187 (2006)
- Pietta PG, "Flavonoids as antioxidants", 63, pp.1035~1042 (2000)
- New York, "Weiter JJ. Phototoxic changes in the retina. In D. Miller Eds. Clinical Light Damage to the Eye", pp.79~125 (1987)
- Bynoe LA, "Rosea GM. Light-dependent generation of superoxide from human erythrocytes. Photochem Photobiol 1992", pp.353~6
- Kielbassa C, "Wavelength dependence of oxidative DNA damage induced by UV and visible light", 18(4), pp.811~816 (1997)
- Osborne NN, "Retinal ischemia: mechanisms of damage and potential therapeutic strategies", 23, pp.91~147 (2004)
- Izzotti A, "The role of oxidative stress in glaucoma", 612, pp.105~114 (2006)
- Tezel G, "Oxidative stress in glaucomatous neurodegeneration : Mechanisms and consequences", 25, pp.490~513 (2006)
- Osborne NN, "Neuroprotection in relation to retinal ischemia and relevance to glaucoma", 43, pp.S102~S128 (1999)
- Flammer J, "Optic nerve blood-flow abnormalities in glaucoma", 17, pp.267~289 (1998)
- Chen JH, "Green tea polyphenols prevent toxin-induced hepatotoxicity in mice by down-regulating inducible nitric oxide-derived prooxidants", 80, pp.742~751 (2004)
- Tedeschi E, "Antiinflammatory action of EGCG, the main component of green tea, through STAT-1 inhibition", 973, pp.435~437 (2002)
- Lorenz M, "A constituent of green tea, epigallocatechin-3-gallate, activates endothelial nitric oxide synthase by a phosphatidylinositol-3-OH-kinase-, cAMP-dependent protein kinase-, and Akt-dependent pathway and leads to endothelial-dependent vasorelaxation", 279, pp.6190~6195 (2004)
- Delaney Y, "Vasospasm in glaucoma: Clinical and laboratory aspects", 83, pp.406~414 (2006)
