Biochemical and Molecular Analysis of OsPAP1: A Phosphate Starvation Induced Purple Acid Phosphatase Gene from Rice
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purple acid phosphatase is important for phosphorus remobilization in plants, but its role in plant adaptation to low phosphorus availability is not known. The cDNA encoding O. sativa purple acid phosphatase (OsPAP1) has 1008 bp with an open reading frame of 335 amino acid residues. The amino acid sequence of OsPAP1 cDNA shows of 50-51% identity with other plant purple acid phosphatases. OsPAP1 was expressed in rice plants and in cell cultures in the absence of phosphate (Pi). The expression was organ-specific with the strongest expression in Pi-deprived roots. Functional expression of the OsPAP1 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analysis. OsPAP1 overexpression lines had higher phosphatase activity than wild-type. Overexpression of OsPAP1 in Arabidopsis plants resulted in increased Pi accumulation under Pi sufficient condition. These results show that the OsPAP1 gene represents more efficient Pi uptake and can be used to develop new transgenic dicotyledonous plants.
Abstract
Purple acid phosphatase is important for phosphorus remobilization in plants, but its role in plant adaptation to low phosphorus availability is not known. The cDNA encoding O. sativa purple acid phosphatase (OsPAP1) has 1008 bp with an open reading frame of 335 amino acid residues. The amino acid sequence of OsPAP1 cDNA shows of 50-51% identity with other plant purple acid phosphatases. OsPAP1 was expressed in rice plants and in cell cultures in the absence of phosphate (Pi). The expression was organ-specific with the strongest expression in Pi-deprived roots. Functional expression of the OsPAP1 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analysis. OsPAP1 overexpression lines had higher phosphatase activity than wild-type. Overexpression of OsPAP1 in Arabidopsis plants resulted in increased Pi accumulation under Pi sufficient condition. These results show that the OsPAP1 gene represents more efficient Pi uptake and can be used to develop new transgenic dicotyledonous plants.
참고문헌 (18)
- Baldwin JC, "LEPS2, a phosphorus starvation-induced novel acid phosphatase from tomato", Plant Physiol, 125, pp.728~737 (2001)
- Clough SJ, "Floral dip: a simplified method for Agrobacterium mediated transformation of Arabidopsis thaliana", Plant J, 16, pp.735~743 (1998)
- del Pozo JC, "A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilising", oxidative stress conditions, 1999, pp.19, 579~589
- Hegeman CE, "A novel phytase with similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings", Plant Physiol, 126, pp.1598~1608 (2001)
- Holford, I.C.R., "Soil phosphorus : its measurement and its uptake by plants", Aust J of soil research, 35, pp.227~239 (1997)
- Hur YJ, "A phosphate starvationinduced acid phosphatase from Oryza sativa: phosphate regulation and transgenic expression", Biotechnol Lett, 29, pp.829~835 (2007)
- Hur YJ, "Molecular characterization of OsPAP2: transgenic expression of a purple acid phosphatase up-regulated in phosphate-deprived rice suspension cells", Biotechnol Lett, 32(1), pp.163~170 (2010)
- Miura K, "The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses", Proc Natl Acad Sci U S A, 102(21), pp.7760~7765 (2005)
- Klabunde T, "Mechanism of FeIII -ZnII purple acid phosphatase based on crystal structures", J Mol Biol, 259, pp.737~748 (1996)
- Li D, "Purple acid phosphatases of Arabidopsis thaliana", J Biol Chem, pp.27772~27781 (2002)
- M. Nanamori, "Low Phosphorus Tolerance Mechanisms: Phosphorus Recycling and Photosynthate Partitioning in the Tropical Forage Grass, Brachiaria Hybrid Cultivar Mulato Compared with Rice", Plant Cell Physiol, 45(4), pp.460~469 (2004)
- Olczak M, "Plant purple acid phosphatases –genes, structures and biological function", Acta Biochim Pol, 50(4), pp.1245~1256 (2003)
- Raghothama KG, "Phosphate acquisition", Ann Review of Plant Physiol, 50, pp.665~693 (1999)
- Thompson JD, "The ClustalX windows interface : flexible strategies for multiple sequence alignment aided by quality analysis tools", Nucleic Acids Res, 24, pp.4876~4882 (1997)
- Yang, Y.¬S., "Circular dichroism and magnetic circular dichroism studies of the mixed¬valence binuclear non¬heme iron active site in uteroferrin and its anion complexes", J Am Chem Soc, 119, pp.11832~11842 (1997)
- Yi-Hong Wang, "Rapid Induction of Regulatory and Transporter Genes in Response to Phosphorus, Potassium, and Iron Deficiencies in Tomato Roots. Evidence for Cross Talk and Root", Rhizosphere-Mediated Signals, 2002, pp.130, 1361~1370
- Yu SM, "Metabolic derepression of alpha-amylase gene expression in suspension-cultured cells of rice", J Biol Chem, 266, pp.21131~21137 (1991)
- Zimmermann P, "Differential expression of three purple acid phosphatases from potato", Plant Biol, 6, pp.519~528 (2004)