용해된 시멘트 분말이 붕어에 미치는 영향
The Effect of Dissolved Cement Powder on Carassius auratus
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In this study, we studied the effect of dissolved cement powder on Carassius auratus byanalysis of the morphophysiological changes. The gill exposed to dissolved cement powder showedthe thickened primary lamellae and the activity of chloride cells and mucous cells was also significantlyincreased and the proliferation, separation and clubbing of gill filament was observed inthe secondary lamellae. In the kidney tissue, the space in Bowman’s capsule was widen and thearrangement of dermis was irregular due to the thinned epidermis in the integument tissue. Theactivities of antioxidant enzymes and LDH tended to increase with the duration of cement exposure. It was confirmed that the up-regulated proteins were identified as involved in glycolysis andenergy metabolism and down-regulated proteins were myofibrillar proteins which were involvedin muscle contraction by the cement exposure to the integument. With these results, dissolvedcement powder was thought to be a big threat to the survival of the fish because it causes the morphologicalchanges and weakens the physiological activity in C. auratus tissues.
Abstract
In this study, we studied the effect of dissolved cement powder on Carassius auratus byanalysis of the morphophysiological changes. The gill exposed to dissolved cement powder showedthe thickened primary lamellae and the activity of chloride cells and mucous cells was also significantlyincreased and the proliferation, separation and clubbing of gill filament was observed inthe secondary lamellae. In the kidney tissue, the space in Bowman’s capsule was widen and thearrangement of dermis was irregular due to the thinned epidermis in the integument tissue. Theactivities of antioxidant enzymes and LDH tended to increase with the duration of cement exposure. It was confirmed that the up-regulated proteins were identified as involved in glycolysis andenergy metabolism and down-regulated proteins were myofibrillar proteins which were involvedin muscle contraction by the cement exposure to the integument. With these results, dissolvedcement powder was thought to be a big threat to the survival of the fish because it causes the morphologicalchanges and weakens the physiological activity in C. auratus tissues.
참고문헌 (27)
- Aebi H., "Catalase in vitro. pp. 121-126. In Methods in Enzymology" (1984), Academic Press
- Alscher RG., "Antioxidants in higher plants", pp.31~58 (1993), CRC Press
- APHA., "Standard methods for the examination of water and wastewater" (1985), APHA
- Barton BA., "Physiological changes in fish from stress in aquaculture with emphasis on the responses and effects of corticosteroids", Ann. Rev. Fish Dis, 1, pp.3~26 (1991)
- Begum G., "Effect of acute exposure of the oraganophosphate insecticide rogor on some biochemical aspects of Clarias batrachus (Linnaeus)", Environ. Res. Sec, 80A, pp.80~83 (1999)
- Water quality for pond aquaculture, "Boyd CE.", International center for aquaculture and aquatic environments. Alabama Argicultural Experiment Station. Auburn University, ~, pp.Research and development series No, 43 (1998)
- Buono RJ., "Hypoxic repression of lactate dehydrogenase-B in retina", Exp. Eye Res, 69, pp.685~693 (1999)
- Chrousos GP., "Stressors, stress, and neuroendocrine integration of the adaptive response", Ann. N. Y. Acad. Sci, 851, pp.311~335 (1998)
- Dalton DA., "Correlations between the ascorbate-glutathione pathway and effectiveness in legume root nodules", Physiol. Plant, 87, pp.365~370 (1993)
- Davis BJ., "Disc electrophoresis-II. Method and application to human serum proteins", Ann. N. Y. Acad. Sci, 121, pp.404~427 (1964)
- Evans DH., "The fish gill : Site of action and model for toxic effects of environmental pollutants", Environ. Health Perspect, 71, pp.47~58 (1987)
- Flohe L., "Methods in enzymatic analysis", pp.114~130 (1984), Academic Press
- Gill TS., "Cadmium nephropathy in a freshwater fish, Puntius conchonius Hamilton", Ecotoxicol. Environ. Safe, 18, pp.165~172 (1989)
- Hosoya S., "Changes in free and total plasma cortisol levels in juvenile haddock (Melanogrammus aeglefinus) exposed to long-term handling stress", Comp. Biochem. Physiol, 146A, pp.78~86 (2007)
- Jee JH., "Responses of cypermethrin-induced stress in haematological parameters of Korean rockfish, Sebastes schlegeli(Hilgendorf)", Aquacul. Res, 36, pp.898~905 (2005)
- 김정곤, "유해물질 노출로 인한 분자·생화학적 바이오마커와 담수 어류에 대한 현장 적용성", 한국환경보건학회지, 36(5), pp.418~434 (2010), 한국환경보건학회
- Lee CK., "Studies on the effects of copper on the lactate dehydrogenase and esterase isozymes in various tissues of Carassius carassius", Korean J. Zool, 2, pp.79~96 (1973)
- 이정열, "빙어(Hypomesus olidus)의 폐사에 미치는 시멘트의 급성독성", 환경생물, 23(1), pp.89~92 (2005), 한국환경생물학회
- Mallatt J., "Fish gill structural changes induced by toxicants and other irritants : a statistical review", Can. J. Fish Aqu. Sci, 42, pp.630~648 (1985)
- McCord JM., "Superoxide dismutase an enzymic function erythrocuprotein(Hemocuprotein)", J. Biol. Chem, 244, pp.6649~6055 (1969)
- Mommsen TP., "Cortisol in teleosts : dynamics, mechanisms of action, and metabolic regulation", Revi. Fish Biol. Fish, 9, pp.211~268 (1999)
- Naaby-Hansen S., "Proteomics-post-genomic cartography to understand gene function", Trends Pharmacol. Sci, 22, pp.376~384 (2001)
- Reid SG., "The adrenergic stress response in fish: Control of catecholamine storage and release", Comp. Biochem. Physiol, 120C, pp.1~27 (1998)
- Roy RN., "The dissociation constants of carbonic acid in seawater at salinities 5 to 45 and temperatures 0 to 45C", Mar. Chem, 44, pp.249~267 (1993)
- Schnaitman C., "The submitochondrial localization of monoamine oxidase. An enzymatic marker for the outer membrane of rat liver mitochondria", J. Cell Biol, 32, pp.719~735 (1967)
- Schwaiger J., "Chronic toxicity of nonylphenol and ethinylestradiol : haematological and histopathological effects in juvenile common carp, Cyprinus carpio", Aquat. Toxicol, 51, pp.69~78 (2000)
- Whitt GS., "Developmental genetics of the lactate dehydrogenase isozymes of fish", J. Exp. Zool, 175, pp.1~35 (1970)
