Little Leaf and Yellowing Symptoms on Castanea crenata are Associated with Phytoplasma in Korea
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
For unknown reasons, a few trees in a private chestnut orchard in Icheon si, Gyunggi-do suffered leaf chlorosis and growth decline. Based on symptoms, phytoplasma was a probable cause. Leaf samples were collected from two symptomatic and non-symptomatic trees in the orchard for phytoplasma detection. An amplicon of about 1.2 bp size was obtained from both symptomatic trees by PCR with the universal 16S rDNA primers. Sequences of these amplicons were found to have 99% nucleotide sequence identity to the corresponding genomic region of 16SrIII (X-disease group). More than 100 phytoplasma isolates, such as Candidatus phytoplasma pruni, Milkweed yellows phytoplasma, Goldenrod yellows phytoplasma, Tsuwabuki witches'-broom phytoplasma, Candidatus Phytoplasma trifolii, etc. were involved in the list. Phylogenetic analysis revealed that the sequence obtained in this study closely clustered with Candidatus phytoplasma groups. While one of the amplicons shared 91% identity with the Candidatus phytoplasma castaneae, the other shared only 47%. It needs further analysis and investigation to determine the exact taxonomy. Meanwhile, based on the analysis of the sequences, chlorosis, and small leaves were associated with phytoplasma.
Abstract
For unknown reasons, a few trees in a private chestnut orchard in Icheon si, Gyunggi-do suffered leaf chlorosis and growth decline. Based on symptoms, phytoplasma was a probable cause. Leaf samples were collected from two symptomatic and non-symptomatic trees in the orchard for phytoplasma detection. An amplicon of about 1.2 bp size was obtained from both symptomatic trees by PCR with the universal 16S rDNA primers. Sequences of these amplicons were found to have 99% nucleotide sequence identity to the corresponding genomic region of 16SrIII (X-disease group). More than 100 phytoplasma isolates, such as Candidatus phytoplasma pruni, Milkweed yellows phytoplasma, Goldenrod yellows phytoplasma, Tsuwabuki witches'-broom phytoplasma, Candidatus Phytoplasma trifolii, etc. were involved in the list. Phylogenetic analysis revealed that the sequence obtained in this study closely clustered with Candidatus phytoplasma groups. While one of the amplicons shared 91% identity with the Candidatus phytoplasma castaneae, the other shared only 47%. It needs further analysis and investigation to determine the exact taxonomy. Meanwhile, based on the analysis of the sequences, chlorosis, and small leaves were associated with phytoplasma.
참고문헌 (17)
- Bertaccini A, "Phytoplasma and phytoplasma diseases : a review of recent research", Phytopathol Mediterr, 48, pp.355~378 (2009)
- Bertin S, "Molecular identification of phytoplasma vector species", Methods Mol Biol, 938, pp.87~108 (2013)
- Davis RE, "‘Candidatus Phytoplasma pruni’, a novel taxon associated with X-disease of stone fruits, Prunus spp. : multilocus characterization based on 16S rRNA, secY, and ribosomal protein genes", Int J Syst Evol Microbiol, 63(Pt 2), pp.766~776 (2013)
- Chestnut Production Statistics, "FAOSTAT", FAO, ~ (2000)
- Girsova NV, "Diverse phytoplasmas associated with leguminous crops in Russia", Eur J Plant Pathol, 149, pp.599~610 (2017)
- Han SS, "Association of phytoplasma with chestnut(Castanea crenata Sieb. et Zucc. )little leaf disease in Korea", Korean J Mycoplasmol, 8, pp.48~54 (1997)
- Jung HY, "‘Candidatus Phytoplasma castaneae’, a novel phytoplasma taxon associated with chestnut witches’ broom disease", Int J Syst Evol Microbiol, 52(Pt 5), pp.1543~1549 (2002)
- 정희영, "Current Status of Phytoplasmas and their Related Diseases in Korea", The Plant Pathology Journal, 28(3), pp.239~247 (2012), 한국식물병리학회[DOI]
- Lee HB, "First Report of Powdery Mildew Caused by Erysiphe alphitoides on Quercus acutissima in Korea", Plant Dis, 99, pp.889~ (2015)
- Lee IM, "Phytoplasma : phytopathogenic mollicutes", Annu Rev Microbiol, 54, pp.221~255 (2000)
- 이종규, "Characteristics of Hypovirulent Strains of Chestnut Blight Fungus, Cryphonectria parasitica, Isolated in Korea", Mycobiology, 34(2), pp.61~66 (2006), 한국균학회
- Luan JB, "Global analysis of the transcriptional response of whitefly to tomato yellow leaf curl China virus reveals the relationship of coevolved adaptations", J Virol, 85, pp.3330~3340 (2011)
- Marcone C, "On some significant phytoplasma diseases of forest trees : an update", Forests, 12, pp.408~ (2021)
- Sixty Years of Forest Tree Breeding Korea, "National Institute of Forest Science", National Institute of Forest Science, 509~ (2017)
- Perez-Lopez E, "Novel phytoplasma strains of X-disease group unveil genetic markers that distinguish North American and South American geographic lineages within subgroups 16SrIII-J and 16SrIII-U", Ann Appl Biol, 171, pp.405~416 (2017)
- Tseng YW, "First Report on the Association of a 16SrII-A Phytoplasma with Sesame(Sesamum indicum)Exhibiting Abnormal Stem Curling and Phyllody in Taiwan", Plant Dis, 98, pp.990~ (2014)
- Valiunas D, "Establishment of a new phytoplasma subgroup, 16SRI-Q, to accommodate a previously undescribed phytoplasma found in diseased cherry in Lithuania", J Plant Pathol, 91, pp.71~75 (2009)