Activation of Toll-like Receptors 1, 2, 4, 5, and 7 on Human Melanocytes Modulate Pigmentation
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초록
Human melanocytes are not simply pigment-producing cells. It may be part of the inflammatory response, during which the pigmentary system may produce more melanin or suppress melanization. Toll-like receptors (TLRs) have been implicated in both innate host defense against pathogens and inflammatory response. Therefore, it may be possible that activation of TLRs in melanocytes may play a role in the modulation of melanogenesis. In this study, we investigated whether normal human melanocytes expressed TLRs and analyzed pigmentation changes upon TLR stimulation. The expression of TLR1∼10 mRNA in cultured human melanocyte was analyzed using RT-PCR, Western blotting and immunocytochemistry. Human melanocytes constitutively express mRNA and protein for TLR2, 3, 4, 5, 7, 9 and 10. Stimulation of TLR1/2 and 4 with Pam3CSK4 and lipopolysaccharide induced pigmentation of melanocytes. Activation of TLR5 and 7 with flagellin and imiquimod treatments reduced pigmentation of melanocytes and zebrafish. In summary, the results provided evidence for TLRs expression in normal human melanocytes. It is speculated that a response of melanocyte to TLR ligands may play a role in the pigmentary change in the skin. (Ann Dermatol 22(4) 486∼489, 2010)
Abstract
Human melanocytes are not simply pigment-producing cells. It may be part of the inflammatory response, during which the pigmentary system may produce more melanin or suppress melanization. Toll-like receptors (TLRs) have been implicated in both innate host defense against pathogens and inflammatory response. Therefore, it may be possible that activation of TLRs in melanocytes may play a role in the modulation of melanogenesis. In this study, we investigated whether normal human melanocytes expressed TLRs and analyzed pigmentation changes upon TLR stimulation. The expression of TLR1∼10 mRNA in cultured human melanocyte was analyzed using RT-PCR, Western blotting and immunocytochemistry. Human melanocytes constitutively express mRNA and protein for TLR2, 3, 4, 5, 7, 9 and 10. Stimulation of TLR1/2 and 4 with Pam3CSK4 and lipopolysaccharide induced pigmentation of melanocytes. Activation of TLR5 and 7 with flagellin and imiquimod treatments reduced pigmentation of melanocytes and zebrafish. In summary, the results provided evidence for TLRs expression in normal human melanocytes. It is speculated that a response of melanocyte to TLR ligands may play a role in the pigmentary change in the skin. (Ann Dermatol 22(4) 486∼489, 2010)
참고문헌 (7)
- Plonka PM, "What are melanocytes really doing all day long...?", Exp Dermatol, 18, pp.799~819 (2009)
- Terhorst D, "The role of toll-like receptors in host defenses and their relevance to dermatologic diseases", Am J Clin Dermatol, 11, pp.1~10 (2010)
- Mackintosh JA, "The antimicrobial properties of melanocytes,melanosomes and melanin and the evolution of black skin", J Theor Biol, 211, pp.101~113 (2001)
- Yu N, "Cultured human melanocytes express functional toll-like receptors 2-4,7 and 9", J Dermatol Sci, 56, pp.113~120 (2009)
- Ahn JH, "Human melanocytes express functional Toll-like receptor 4", Exp Dermatol, 17, pp.412~417 (2008)
- Kang HY, "Imiquimod,a Toll-like receptor 7 agonist,inhibits melanogenesis and proliferation of human melanocytes", J Invest Dermatol, 129, pp.243~246 (2009)
- Choi TY, "Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds", Pigment Cell Res, 20, pp.120~127 (2007)